LOCUS       sequence-262763-        2097 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CTAG Nuclear localiation signal SV40 for fusion with CDS12.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Sauret-Gueto S, Frangedakis E, Silvestri L, Rebmann M, Tomaselli M, 
            Markel K, Delmans M, West A, Patron NJ, Haseloff J
  TITLE     Systematic tools for reprogramming plant gene expression in a simple
            model, Marchantia polymorpha.
  JOURNAL   ACS Synth Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511.
  PUBMED    32163700
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511."
COMMENT     Sequence Label: CTAG_NLS-SV40
FEATURES             Location/Qualifiers
     source          1..2097
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     162..180
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(184..227)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     CDS             253..273
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      300..357
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(381..398)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(552..1140)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(632..651)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1323..1417)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1438..2097)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2012..2031
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct
       61 tatttcatta tggtgaaagt tggaacctct tacgtgcccg atcaactcga gtgccacctg
      121 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
      181 agaatttcag ataaaaaaaa tccttagctt tcgctaagga tgatttctgg aattcggtct
      241 cattcgttgc agcctaagaa gaagagaaag gttggaggat aagcttcgag accctgcagt
      301 ccggcaaaaa agggcaaggt gtcaccaccc tgcccttttt ctttaaaacc gaaaagatta
      361 cttcgcgtta tgcaggcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
      421 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
      481 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
      541 ttgctggcgt ttttccacag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
      601 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
      661 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
      721 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
      781 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
      841 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
      901 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
      961 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     1021 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     1081 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     1141 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     1201 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     1261 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ctcgaggctt
     1321 ggattctcac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa atccagatgg
     1381 agttctgagg tcattactgg atctatcaac aggagtccaa gcgagctcga tatcaaatta
     1441 cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg
     1501 gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc
     1561 ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac
     1621 gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa acatattctc
     1681 aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata
     1741 tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc
     1801 agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc
     1861 gtctttcatt gccatacgaa attccggatg agcattcatc aggcgggcaa gaatgtgaat
     1921 aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc
     1981 cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc
     2041 tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt tctccat
//
