LOCUS       sequence-262764-        3853 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PROM for cloning with 5UTR_MpUBE2. PROM+5UTR_MpUBE2 has ubiquitous 
            expression in early satges of gemmae development..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Sauret-Gueto S, Frangedakis E, Silvestri L, Rebmann M, Tomaselli M, 
            Markel K, Delmans M, West A, Patron NJ, Haseloff J
  TITLE     Systematic tools for reprogramming plant gene expression in a simple
            model, Marchantia polymorpha.
  JOURNAL   ACS Synth Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511.
  PUBMED    32163700
REFERENCE   2  (bases 1 to 3853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511."
COMMENT     Sequence Label: PROM_MpUBE2
FEATURES             Location/Qualifiers
     source          1..3853
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     162..180
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(184..227)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     CDS             1698..1709
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      2056..2113
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(2137..2154)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2308..2896)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2388..2407)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3079..3173)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3194..3853)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3768..3787
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct
       61 tatttcatta tggtgaaagt tggaacctct tacgtgcccg atcaactcga gtgccacctg
      121 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
      181 agaatttcag ataaaaaaaa tccttagctt tcgctaagga tgatttctgg aattcggtct
      241 caggagacgg ggcggataaa aattttggcg gtgtttccag ccctccaacc actaaatgcg
      301 aaaccgccca acccaaaacc tccttgcgga tagatctcgg gtacagtgtt taccgtgcgg
      361 ggggaaacgt gcatggcatt attccgacag cctgaactca ggttgggggc cgagcgggga
      421 gaaggccgcg gtgcggtcat gacaagggaa tcgagtcgca ggacgtggaa gcccaaagtc
      481 ggcgacccct ggcttcggaa atcattgcgt aatccggact tccctgcaag gcccggcacg
      541 gccatgatgg cacatgggtc attcggtggg ccgggggtgg tcaccggact cgaacgtgag
      601 aatagcattt ctggccatag gctcgaacca ggcgatcgta ggtgtggaca tgctaccggc
      661 agcgggatgg ggaccgtcga aaccgggcgg acggaccggc aggtgcggca gtcggggccc
      721 gaaagaggac gacgagggcc gggaagaact gggacgacgg gggtgacgcg tcgcgtagcg
      781 tggagaagca aggccccgag cgacgggacg acgaggccgg acgtgagtct ctgcagtcct
      841 ccgcaaggca cgaacgaagg gaacgacgag cgctgaaagg gtcgaacaag tggactgtcg
      901 ctgcggccag gatcgacggg gaccggggct agctagatcg gggcgactga ccgactgacc
      961 ctctgaatgt ccgtccatct ggaagggaat ttcggagcag gattaaatag ctacacgacc
     1021 caggactgtg gccccagagc gtgccgagcc tcagcgcccc actcacgagg gcgaaaccaa
     1081 acgcatggct cccgtcggcc cagctgtgcc gcgtcggaca cttcgtcatt gattatccgc
     1141 ccgtcgggcc gatggcgcga ccggaacagc atttaccaga ttgttccaga cagcctcggg
     1201 gatttaggtc tagtagctgt ccgagcttgg cgtgcagctt cggttcggtg cccggaagtt
     1261 accagcacca aaacgcgtgg aacttgtagc ctggaggtag ctggcgtagc ggcttacttg
     1321 ttgcggatag aagagagtcc cgcaggagat cgggtggagt gccaccggac cgggtgttcc
     1381 ggtccccatt gaggacagaa cggtagctgg ctcggatgtg tggataaagt tgccgtcgcc
     1441 tgtcgcgatg gatggatgga tggaaggatg gatggagcca gtgattctgg gtgccgagtg
     1501 cggatgtgtc acggggaagg ctgcgaggaa cggcaaaacg tgcctcgacc gtgtaacatg
     1561 acgcaaacgg cagcggggcg gcatgccatc cgccggggcc atgccgcgtg gccgtcggat
     1621 tcgagatcca ccgagccgga gagcccgaag cactgacgga tgaagtccac gccagaaact
     1681 cgggggacgt acgtggtatc gaaggccgag gaggaggaca gagagcggaa tcgggacata
     1741 accgggccaa ccgtccgatc ccgaatccga aggtggaggg actttgcgtc cggagattca
     1801 gagccgtgaa acgcgaaatg cagcgtcaga cgccggaagc ttcgtcgcgc tggatggcgg
     1861 gcgagaagag ggttctgatt tgaccggcgg atgcggatgc gggtgcggat ccgacagtcg
     1921 aatgggaagg gaggactgca attcacggcc acgaccggga gcaggactga ctttgaactg
     1981 gtacggagcg agggtgtgcg ggcggagcat attccgtcca catcgaagtc ggggtcggta
     2041 cttgagaccc tgcagtccgg caaaaaaggg caaggtgtca ccaccctgcc ctttttcttt
     2101 aaaaccgaaa agattacttc gcgttatgca ggcttcctcg ctcactgact cgctgcgctc
     2161 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     2221 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     2281 ccgtaaaaag gccgcgttgc tggcgttttt ccacaggctc cgcccccctg acgagcatca
     2341 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     2401 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     2461 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     2521 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     2581 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     2641 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     2701 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     2761 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     2821 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     2881 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     2941 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     3001 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     3061 tgacagctcg aggcttggat tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct
     3121 gaacaaatcc agatggagtt ctgaggtcat tactggatct atcaacagga gtccaagcga
     3181 gctcgatatc aaattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa
     3241 gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca
     3301 tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt
     3361 tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaga
     3421 cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg
     3481 ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga
     3541 gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc
     3601 atatcaccag ctcaccgtct ttcattgcca tacgaaattc cggatgagca ttcatcaggc
     3661 gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta
     3721 aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa
     3781 atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga
     3841 tttttttctc cat
//
