LOCUS       sequence-262765-        3117 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses opto-Rac1 (BcLOV4-GGGSx2-WT_Rac1-GGGSx2-mCherry in 
            mammalian cells in the pcDNA3.1 backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Berlew EE, Kuznetsov IA, Yamada K, Bugaj LJ, Chow BY
  TITLE     Optogenetic Rac1 engineered from membrane lipid-binding RGS-LOV for 
            inducible lamellipodia formation.
  JOURNAL   Photochem Photobiol Sci. 2020 Mar 1;19(3):353-361. doi: 
            10.1039/c9pp00434c. Epub 2020 Feb 12.
  PUBMED    32048687
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1039/c9pp00434c"; journalName: "Photochem Photobiol Sci"; date: 
            "2020-03-1- 1"; volume: "19"; issue: "3"; pages: "353-361"
COMMENT     Sequence Label: opto-Rac1
FEATURES             Location/Qualifiers
     source          1..3117
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2410..3117
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2555..2573)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2823..2842
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atggccacag acgcaatcga ccattactac cagaacagag acggcccccc ttccaggagc
       61 tttagcgacg aatacgactc atacaataag acagcccctc tgccacctct gagcgcctgc
      121 ccagagcgcg cccagacccc taacagcaag agaatcccca tcacacccaa ttccgccaag
      181 agcacctccg agtctcctcg gagaatcaca ttctctcgcg acgcaggcag ctccaccaag
      241 ggcagcaccc agacaagccc atccaaccag ggctccgtgt ctagccggaa tggctctaac
      301 ggaaatggcc tgggcctgtc ctctgccggc aacttcgccg atttctttag ctccgaggtg
      361 ttttatatcg tgctgcacaa tcccaccaca gcccacagat tcctgaagtt ttgccagagc
      421 agggcctgtt ccgagaacct ggagtttctg cagaagatcg acgagtacaa taggctgatc
      481 gatgaggcca cacgcctgct gtctaccatc tacagcacat acgtgagcag cgaggcccct
      541 aagcagatca acatctctaa tagccacgcc aggcgcctga gccacgacat caagggagca
      601 acccacaacg tgctgcccgg cctggaggat atcttcaatg gctcccagga gcagatcgag
      661 aagctgctgg cctctgacct gtatcctaga tttgtgaagc accaggtgac cgcatccgcc
      721 acaatggccc tggcaaacga caaggagagg tacccaggcc tgggcgattg cttctgtctg
      781 acagacccac ggagagccga taatcccatc gtgttcgcaa gcgacggatt cgtgagcgtg
      841 accggctatt cccgctctga tatcatccca aggaactgcc gcttcctgca gggcagcttt
      901 accgaccggc aggccacaaa gaggctgcgc acctccatcg agaatcgcga ggagacagtg
      961 gagctgctgc tgaactatcg gaagaatggc gatccctttt ggaacctgct gtacgtgtcc
     1021 cctctgctgg acggcaatgg cgatgtgaga ttctttctgg gcggccagat caactgttct
     1081 accacaatcc acagccggac cgacgtgctg agaatcctga gcctgaacga cgaggataca
     1141 ggctccttca tcgagggctc taataagacc cccagcgtgc ggtccaagga gtctaacaat
     1201 gcctccaact atggcaagac atccttcttt aagtctttta agaagtacaa ggccaatacc
     1261 gtggagatca gagacgaggc cggcatggag ggcgagctga tcgataggat cggcaagctg
     1321 cagttcaaga cacaggtgga ggagttttac accgcctatt ctaagtacct ggtgctgagc
     1381 tatcagccac agtcccagaa cctgatcgtg aagtacttca gccccggcat cgtggatatg
     1441 ctgaacctga atatgcctaa tggcctggtg gccccaatcg tggacaagga tatcttcaag
     1501 gtgctgacag agcactcccc ttcctctgtg ccacggacct ttaagaacgt ggtgagggaa
     1561 ggcatcaaag caggcagggc cgtgtctgtg gagacaggcc tgctgacagg catcgaggag
     1621 atcaagaagt ctcagaacat cttcagcggc accacagcca atcgggagag aggctggaag
     1681 aaggccgagg agaagtacgt gtgccacttt accccctgta aggatgagat gggcagggtc
     1741 ggctgggtgg tgctgactgt cgctcctaaa ctggaaagat ttgaattcgg tggtggatca
     1801 ggtggaggat ctatgcaagc cataaagtgc gtggttgtag gagacggtgc agtagggaag
     1861 acttgtttgc ttatatctta caccacaaac gcgtttcctg gcgaatacat ccctacggta
     1921 tttgacaact attctgctaa tgtgatggtg gatgggaagc ctgtaaatct tgggctttgg
     1981 gataccgcag ggcaggagga ctatgataga ttgcggccct tgtcttaccc tcaaacagac
     2041 gtttttttga tctgtttctc tctggtcagt cctgcatcct tcgagaatgt aagggctaag
     2101 tggtacccag aggttaggca tcattgtcca aacacgccca ttatattggt tggaaccaag
     2161 ttggacctcc gggatgataa ggatacaatt gagaagctca aggagaagaa gctgactcct
     2221 ataacttacc cacagggact cgcgatggca aaggaaatcg gagcagttaa atacctcgaa
     2281 tgcagtgctc tgacacagcg gggtctgaag acagtctttg atgaggcgat ccgcgccgtt
     2341 ttgtgcccgc cgcctgtaaa aaagcggaaa cgcaagtgtc tgctcggtgg tggatcaggt
     2401 ggaggatcta tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg
     2461 cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
     2521 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc
     2581 cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac
     2641 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag
     2701 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc
     2761 ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac
     2821 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc
     2881 gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac
     2941 tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc
     3001 tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa
     3061 cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaag
//
