LOCUS       sequence-26282-e         687 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Boehm JS, Zhao JJ, Yao J, Kim SY, Firestein R, Dunn IF, Sjostrom SK,
            Garraway LA, Weremowicz S, Richardson AL, Greulich H, Stewart CJ, 
            Mulvey LA, Shen RR, Ambrogio L, Hirozane-Kishikawa T, Hill DE, Vidal
            M, Meyerson M, Grenier JK, Hinkle G, Root DE, Roberts TM, Lander ES,
            Polyak K, Hahn WC
  TITLE     Integrative Genomic Approaches Identify IKBKE as a Breast Cancer 
            Oncogene (Kinase Library Clones)
  JOURNAL   Cell. 2007 Jun 15. 129(6):1065-1079.
  PUBMED    17574021
REFERENCE   2  (bases 1 to 687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Jun 15. 129(6):1065-1079."
COMMENT     Sequence Label: pWZL Neo Myr Flag CKMT1A
FEATURES             Location/Qualifiers
     source          1..687
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      32..41
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             38..79
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             104..127
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    134..158
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cttctctagg cgccggccgg atccagaatt cgccaccatg gggtcttcaa aatctaaacc
       61 aaaggacccc agccagcgcc ggcgcaggat ccgaggttac cttgactaca aagacgatga
      121 cgacaagcaa ttgacaagtt tgtacaaaaa agttggcatg gctggtccct tctcccgtct
      181 gctgtccgcc cgcccgggac tcaggctcct ggctttggcc ggagcggggt ctctagccgc
      241 tgggtttctg ctccgaccgg aacctgtacg agctgccagt gaacgacgga ggctgtatcc
      301 cccgagcgct gagtacccag acctccgaaa gcacaacaac tgcatggcca gtcacctgac
      361 cccagcagtc tatgcacggc tctgcgacaa gaccacaccc actggttgga cgctagatca
      421 gtgtatccag actggcgtgg acaaccctgg ccaccccttc atcaagactg tgggcatggt
      481 ggctggagat gaggagacct atgaggtatt tgctgacctg tttgaccctg tgatccaaga
      541 gcgacacaat ggatatgacc cccggacaat gaagcacacc acggatctag atgccagtaa
      601 aatccgttct ggctactttg atgagaggta tgtattgtcc tctagagtca gaactggccg
      661 aagcatccga ggactcagtc tgcctcc
//
