LOCUS       sequence-262880-        9098 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  L1 in position 3, to clone gRNA target using BbsI, lacZ blue-white 
            screening.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9098)
  AUTHORS   Sauret-Gueto S, Frangedakis E, Silvestri L, Rebmann M, Tomaselli M, 
            Markel K, Delmans M, West A, Patron NJ, Haseloff J
  TITLE     Systematic tools for reprogramming plant gene expression in a simple
            model, Marchantia polymorpha.
  JOURNAL   ACS Synth Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511.
  PUBMED    32163700
REFERENCE   2  (bases 1 to 9098)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511."
COMMENT     Sequence Label: L1_lacZgRNA-Ck3
FEATURES             Location/Qualifiers
     source          1..9098
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    433..457
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     2810..2832
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2824..2841
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2825..2841
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    2842..2898
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2911..2927)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2911..2927)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2924..2946)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2935..2951
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2959..2965,2966..2983,2984..2989))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3004..3025
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_RNA        3087..3162
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(3174..3198)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3221..3245
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4546..5175
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5604..6677
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6743..6937
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7173..7195)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7281..7421
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7436..7453)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7607..8195)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7687..7706)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8283..9098)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(8283..9098)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     9006..9025
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 aacagaactt attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta
       61 attataacaa gacgaactcc aattcactgt tccttgcatt ctaaaacctt aaataccaga
      121 aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga
      181 ttttgaaacc gcggtgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta
      241 ccctccgcga gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag
      301 catcggtaac atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg
      361 gactgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg
      421 ttggctggct ggtggcagga tatattgtgg tgtaaacata acgaattgct cttcatacag
      481 gagtattcat tcaaaagaga tttttaaaga tcaatttttc tttctcataa gtctaatacg
      541 tcatgataat aactttaaaa aaaattgaac tcgttaaaaa atttgatcaa attttaattt
      601 attattagat catatattac atatcagttt cataatgtta tactaagatc tttcatatat
      661 attttaaccc acgagacaaa atatgtttat atctttattt atttaaatat tttttagctc
      721 aaaacatcgc aaactcagct atcataatgc tttttaatga aaaaaacaaa ttttcctatc
      781 atactctcat tgtttataat tttttagaaa aaaaacttga agataaaata acaacaataa
      841 tagtttcaaa agcacatttt tgttgtaaat ttcaagaaaa atacaatcat attactgatt
      901 gactacaaaa atttgtaaat taatccaggc tacatgttta agatgtgtca aaactgcatc
      961 atctacatct cattttaatt tttctaaact acatacatag tatcaataac aattgaacaa
     1021 tgctagttaa tgtgttatac tcattagctt gtaatatatc ttcaacttga tgaatcggat
     1081 tgatcataaa atttcacaaa cttatgtgct gattaataaa aaatataatc agatttttca
     1141 tgaataatta agataaataa cacatgcaag tctcaattag aatttaacaa tctgactata
     1201 aaaagtcggt ttatcttgat tttcacaaaa atttaacata gtttcaaggt gaatgggctt
     1261 ttattttaat ccaatcaact atttgacaca tacatcttgc aaatttattt attttttatg
     1321 gaaaaaaaca tgatttatgt ttttctttga gaaagtatat tgtggattgt caaaaaaaac
     1381 ctatggaaga tttatatgta agagttaaaa atttattttc aaatatgtca acaatccaca
     1441 atatcgaaga aaattttcaa aaaatcatca catatgatgt aaatataata tgaatatgtg
     1501 caatcttatt tcattttact ctgaaaactt aatttctttt tatagagcat tctgtactta
     1561 ctggtgtcaa atatacagat aaaatgcagt ccataacgta tcactctata agcaaaatca
     1621 taaacttcgt gatctctcac agttcaaacg agaactatag aaacccttca actagcaaaa
     1681 aactcacaac tttctattcc aatatcttta attgtctgac gatttacaaa tagaaacgaa
     1741 aaccagacac acacacacac gcaaactaca aagtaaaaac acaaccacct gacattagac
     1801 agtgagatta ttaaacatcc taaactaaaa aacaaacaaa ttaattctct atgtacaagt
     1861 ccccatcgtg atcaattttt gcatcccaat atagtggcgg ttagtataag aggaccttcg
     1921 tctattcact ctccatgttt gtcaaactat agtacagtac atatatgtat gggaacaaat
     1981 tccgaacact ctagtacact cctcaacgca agttctatct catatctctg caagagctta
     2041 ttccaagaaa ctttcttgtg cttctatgca tgcccaggta ccttaagtcg cgcgcatagt
     2101 cacttcacag ccttttcagt tctccctttg atggctcaga gtgacgcaac tgcacaaaaa
     2161 taagaaaaaa gtcgaagcgt cacgagagat gtatccggct agtttccttg gagttcggat
     2221 tgctctcttt ctctgtctcc ccgaaccata tggaatttat gaactgctgt tcactagtca
     2281 gtcacttgtt tcagaacttt tgagtggtcg atttatccac cttcttgttt cttcctgggt
     2341 ccagaatctg acttcttgag acaacaaaca gtgcacagtg gatcgaagaa tgaccacagt
     2401 tgagacacgt ggcgctggcc tcatttattg caccatacgt ttagtgaaga actgaataat
     2461 ggtggatata aagcagagtt gcacccagcc tctcgatgtc ttccacagct tgtctgtaag
     2521 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     2581 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     2641 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcatcc gccattcagg
     2701 ctgcgcaact gttgggaagg gcgatcggcg caggcctctt cgctattacg ccagctggcg
     2761 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
     2821 cgttgtaaaa cgacggccag tgaattcgag ctcggtaccc ggggatcctc tagagtcgac
     2881 ctgcaggcat gcaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     2941 tccgctcaca attccacaca acatacgagc cggaagcata tagtgtaaag cctggggtgc
     3001 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     3061 aaacctgtcg tgccagctgg gaagacgttt tagagctaga aatagcaagt taaaataagg
     3121 ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gctttttttc tagacccagc
     3181 tttcttgtac aaagttggcc gctacagtga agagcaattc tgacaggata tattggcggg
     3241 taaacctaag agaaaagagc gtttattaga ataatcggat atttaaaagg gcgtgaaaag
     3301 gtttatccgt tcgtccattt gtatgtgcat gccaaccaca gggttcccct cgggatcaaa
     3361 gtactttgat ccaacccctc cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc
     3421 cgtcatctga aaacgacatg tcgcacaagt cctaagttac gcgacaggct gccgccctgc
     3481 ccttttcctg gcgttttctt gtcgcgtgtt ttagtcgcat aaagtagaat acttgcgact
     3541 agaaccggag acattacgcc atgaacaaga gcgccgccgc tggcctgctg ggctatgccc
     3601 gcgtcagcac cgacgaccag gacttgacca accaacgggc cgaactgcac gcggccggct
     3661 gcaccaagct gttttccgag aagatcaccg gcaccaggcg cgaccgcccg gagctggcca
     3721 ggatgcttga ccacctacgc cctggcgacg ttgtgacagt gaccaggcta gaccgcctgg
     3781 cccgcagcac ccgcgaccta ctggacattg ccgagcgcat ccaggaggcc ggcgcgggcc
     3841 tgcgtagcct ggcagagccg tgggccgaca ccaccacgcc ggccggccgc atggtgttga
     3901 ccgtgttcgc cggcattgcc gagttcgagc gttccctaat catcgaccgc acccggagcg
     3961 ggcgcgaggc cgccaaggcc cgaggcgtga agtttggccc ccgccctacc ctcaccccgg
     4021 cacagatcgc gcacgcccgc gagctgatcg accaggaagg ccgcaccgtg aaagaggcgg
     4081 ctgcactgct tggcgtgcat cgctcgaccc tgtaccgcgc acttgagcgc agcgaggaag
     4141 tgacgcccac cgaggccagg cggcgcggtg ccttccgtga ggacgcattg accgaggccg
     4201 acgccctggc ggccgccgag aatgaacgcc aagaggaaca agcatgaaac cgcaccagga
     4261 cggccaggac gaaccgtttt tcattaccga agagatcgag gcggagatga tcgcggccgg
     4321 gtacgtgttc gagccgcccg cgcacctctc aaccgtgcgg ctgcatgaaa tcctggccgg
     4381 tttgtctgat gccaagctgg cggcctggcc ggccagcttg gccgctgaag aaaccgagcg
     4441 ccgccgtcta aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc
     4501 gtatatgatg cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa ggttatcgct
     4561 gtacttaacc agaaaggcgg gtcaggcaag acgaccatcg caacccatct agcccgcgcc
     4621 ctgcaactcg ccggggccga tgttctgtta gtcgattccg atccccaggg cagtgcccgc
     4681 gattgggcgg ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg
     4741 attgaccgcg acgtgaaggc catcggccgg cgcgacttcg tagtgatcga cggagcgccc
     4801 caggcggcgg acttggctgt gtccgcgatc aaggcagccg acttcgtgct gattccggtg
     4861 cagccaagcc cttacgacat atgggccacc gccgacctgg tggagctggt taagcagcgc
     4921 attgaggtca cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc
     4981 acgcgcatcg gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc cattcttgag
     5041 tcccgtatca cgcagcgcgt gagctaccca ggcactgccg ccgccggcac aaccgttctt
     5101 gaatcagaac ccgagggcga cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa
     5161 tcaaaactca tttgagttaa tgaggtaaag agaaaatgag caaaagcaca aacacgctaa
     5221 gtgccggccg tccgagcgca cgcagcagca aggctgcaac gttggccagc ctggcagaca
     5281 cgccagccat gaagcgggtc aactttcagt tgccggcgga ggatcacacc aagctgaaga
     5341 tgtacgcggt acgccaaggc aagaccatta ccgagctgct atctgaatac atcgcgcagc
     5401 taccagagta aatgagcaaa tgaataaatg agtagatgaa ttttagcggc taaaggaggc
     5461 ggcatggaaa atcaagaaca accaggcacc gacgccgtgg aatgccccat gtgtggagga
     5521 acgggcggtt ggccaggcgt aagcggctgg gttgcctgcc ggccctgcaa tggcactgga
     5581 acccccaagc ccgaggaatc ggcgtgagcg gtcgcaaacc atccggcccg gtacaaatcg
     5641 gcgcggcgct gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc
     5701 aacgcatcga ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc
     5761 gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg
     5821 gcgacgagca accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc
     5881 gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg
     5941 tgatccgcta cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg
     6001 ccagtgtgtg ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga
     6061 accgataccg ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg
     6121 acgtactcaa gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa
     6181 cctgcattcg gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg
     6241 gccgcctggt gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga
     6301 gcgaaaccgg gcggccggag tacatcgaga tcgagctagc tgattggatg taccgcgaga
     6361 tcacagaagg caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc
     6421 ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca
     6481 gatggttgtt caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct
     6541 gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg
     6601 aggcggggca ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag
     6661 catccgccgg ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa
     6721 aaggtcgaaa aggactcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca
     6781 ttgggaaccg gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca
     6841 tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac
     6901 ttattaaaac tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg
     6961 aagtgctgca aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc
     7021 gtcggcctat cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac
     7081 cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat caaggcaccc
     7141 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggtgacg
     7201 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     7261 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat
     7321 actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     7381 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcatcc gcttcctcgc
     7441 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     7501 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     7561 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     7621 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     7681 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     7741 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     7801 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     7861 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     7921 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     7981 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     8041 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     8101 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     8161 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     8221 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg cattctaggt
     8281 gattagaaaa actcatcgag catcaaatga aactgcaatt tattcatatc aggattatca
     8341 ataccatatt tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc gaggcagttc
     8401 cataggatgg caagatcctg gtatcggtct gcgattccga ctcgtccaac atcaatacaa
     8461 cctattaatt tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc atgagtgacg
     8521 actgaatccg gtgagaatgg caaaagttta tgcatttctt tccagacttg ttcaacaggc
     8581 cagccattac gctcgtcatc aaaatcactc gcatcaacca aaccgttatt cattcgtgat
     8641 tgcgcctgag cgagtcgaaa tacgcgatcg ctgttaaaag gacaattaca aacaggaatc
     8701 gaatgcaacc ggcgcaggaa cactgccagc gcatcaacaa tattttcacc tgaatcagga
     8761 tattcttcta atacctggaa tgctgttttc cctgggatcg cagtggtgag taaccatgca
     8821 tcatcaggag tacggataaa atgcttgatg gtcggaagag gcataaattc cgtcagccag
     8881 tttagtctga ccatctcatc tgtaacatca ttggcaacgc tacctttgcc atgtttcaga
     8941 aacaactctg gcgcatcggg cttcccatac aatcggtaga ttgtcgcacc tgattgcccg
     9001 acattatcgc gagcccattt atacccatat aaatcagcat ccatgttgga atttaatcgc
     9061 ggccttgagc aagacgtttc ccgttgaata tggctcat
//
