LOCUS       sequence-262887-        4452 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4452)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4452)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC6A19
FEATURES             Location/Qualifiers
     source          1..4452
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2577..2676)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2693..2712)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2694..2712)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2717..2733)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2717..2733)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2835..2854)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2846..3655
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2913..2932)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3802..4390
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4291..4310
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatggtga ggctggtgct gccaaaccca ggactggacg caaggatccc
      721 atccctggcc gagctggaga ccatcgagca ggaggaggcc agctccaggc ccaagtggga
      781 taacaaggcc cagtacatgc tgacatgcct gggcttttgc gtgggactgg gaaacgtgtg
      841 gaggttccct tacctgtgcc agagccacgg aggaggagcc tttatgatcc cattcctgat
      901 cctgctggtg ctggagggca tccccctgct gtacctggag tttgccatcg gacagcggct
      961 gaggagagga tccctgggcg tgtggtctag catccaccca gccctgaagg gactgggact
     1021 ggcctctatg ctgaccagct tcatggtggg cctgtactat aacacaatca tcagctggat
     1081 catgtggtac ctgtttaatt ctttccagga gcctctgcca tggagcgact gccctctgaa
     1141 cgagaatcag accggctatg tggacgagtg tgcccggtcc tctccagtgg attacttttg
     1201 gtatagagag accctgaata tctccacatc tatcagcgat tccggctcta tccagtggtg
     1261 gatgctgctg tgcctggcct gtgcctggtc tgtgctgtat atgtgcacca tccggggcat
     1321 cgagaccaca ggcaaggccg tgtacatcac cagcacactg ccctatgtgg tgctgacaat
     1381 cttcctgatc agaggcctga ccctgaaggg cgccacaaac ggcatcgtgt ttctgttcac
     1441 ccccaatgtg acagagctgg cacagcctga cacctggctg gatgcaggag cacaggtgtt
     1501 ctttagcttt tccctggcct tcggcggcct gatctccttt agctcctaca actctgtgca
     1561 caacaattgc gagaaggact ccgtgatcgt gtctatcatc aatggcttca cctccgtgta
     1621 cgtggccatc gtggtgtatt ctgtgatcgg ctttagggcc acacagcggt acgacgattg
     1681 tttcagcacc aacatcctga cactgatcaa tggctttgac ctgcccgagg gcaacgtgac
     1741 acaggagaat ttcgtggaca tgcagcagag gtgcaacgca agcgatcctg cagcatacgc
     1801 acagctggtg tttcagacct gtgatatcaa tgccttcctg tccgaggcag tggagggaac
     1861 aggactggcc tttatcgtgt tcaccgaggc catcacaaag atgcccctga gccctctgtg
     1921 gtccgtgctg ttctttatca tgctgttttg cctgggcctg tctagcatgt tcggcaacat
     1981 ggagggagtg gtggtgcctc tgcaggacct gcgcgtgatc ccacctaagt ggccaaagga
     2041 ggtgctgacc ggcctgatct gtctgggcac attcctgatc ggctttatct tcaccctgaa
     2101 ttccggccag tactggctgt ctctgctgga tagctatgcc ggctccatcc ctctgctgat
     2161 catcgccttt tgcgagatgt tctctgtggt gtacgtgtat ggcgtggacc ggtttaacaa
     2221 ggatatcgag ttcatgatcg gccacaagcc caatatcttt tggcaggtga cctggagagt
     2281 ggtgtcccct ctgctgatgc tgatcatctt cctgttcttt ttcgtggtgg aggtgagcca
     2341 ggagctgaca tactccatct gggatcctgg ctatgaggag ttcccaaagt ctcagaagat
     2401 cagctaccca aactgggtgt atgtggtggt ggtcatcgtg gcaggagtgc caagcctgac
     2461 catcccaggc tacgccatct ataagctgat caggaaccac tgtcagaagc caggcgacca
     2521 ccagggactg gtgtctaccc tgagcacagc ctccatgaat ggcgatctga agtacgaccc
     2581 agctttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga
     2641 acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgata tcccctatag
     2701 tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct
     2761 ctgatgttac attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta
     2821 cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg
     2881 attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg
     2941 gcaatcaggt gcgacaatct atcgcttgta tgggaagccc gatgcgccag agttgtttct
     3001 gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg
     3061 gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc
     3121 atggttactc accactgcga tccccggaaa aacagcattc caggtattag aagaatatcc
     3181 tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat
     3241 tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc
     3301 acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc
     3361 tgttgaacaa gtctggaaag aaatgcataa acttttgcca ttctcaccgg attcagtcgt
     3421 cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg
     3481 tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa
     3541 ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga
     3601 taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga
     3661 attggttaat tggttgtaac actggcagag cattacgctg acttgacggg acggcgcaag
     3721 ctcatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt
     3781 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     3841 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     3901 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     3961 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     4021 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     4081 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     4141 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     4201 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     4261 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     4321 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     4381 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     4441 tgctcacatg tt
//
