LOCUS       sequence-26290-e         754 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Boehm JS, Zhao JJ, Yao J, Kim SY, Firestein R, Dunn IF, Sjostrom SK,
            Garraway LA, Weremowicz S, Richardson AL, Greulich H, Stewart CJ, 
            Mulvey LA, Shen RR, Ambrogio L, Hirozane-Kishikawa T, Hill DE, Vidal
            M, Meyerson M, Grenier JK, Hinkle G, Root DE, Roberts TM, Lander ES,
            Polyak K, Hahn WC
  TITLE     Integrative Genomic Approaches Identify IKBKE as a Breast Cancer 
            Oncogene (Kinase Library Clones)
  JOURNAL   Cell. 2007 Jun 15. 129(6):1065-1079.
  PUBMED    17574021
REFERENCE   2  (bases 1 to 754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Jun 15. 129(6):1065-1079."
COMMENT     Sequence Label: pWZL Neo Myr Flag CSNK1A1L
FEATURES             Location/Qualifiers
     source          1..754
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             43..84
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             109..132
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    139..163
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cactccttct ctaggcgccg gccggatcca gaattcgcca ccatggggtc ttcaaaatct
       61 aaaccaaagg accccagcca gcgccggcgc aggatccgag gttaccttga ctacaaagac
      121 gatgacgaca agcaattgac aagtttgtac aaaaaagttg gcatggcgga cctggaggct
      181 gtgctggccg atgtcagtta cctgatggcc atggagaaga gcaaggcgac cccggccgcc
      241 cgcgccagca agaggatcgt cctgccggag cccagtatcc ggagtgtgat gcagaagtac
      301 cttgcagaga gaaatgaaat aacctttgac aagattttca atcagaaaat tggtttcttg
      361 ctatttaaag atttttgttt gaatgaaatt aatgaagctg tacctcaggt gaagttttat
      421 gaagagataa aggaatatga aaaacttgat aatgaggaag accgcctttg cagaagtcga
      481 caaatttatg atgcctacat catgaaggaa cttctttcct gttcacatcc tttctcaaag
      541 caagctgtag aacacgtaca aagtcattta tccaagaaac aagtgacatc aactcttttt
      601 cagccataca tagaagaaat ttgtgaaagc cttcgaggtg acatttttca aaaatttatg
      661 gaaagtgaca agttcactag attttgtcag tggaaaaacg ttgaattaaa tatccatttg
      721 accatgaatg agttcagtgt gcataggatt attg
//
