LOCUS       sequence-262901-        1014 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express chimeric protein hNABP1 N-terminus and hNABP2 C-terminus in 
            E. coli with a C-terminal His-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Vidhyasagar V, He Y, Guo M, Ding H, Talwar T, Nguyen V, Nwosu J, 
            Katselis G, Wu Y
  TITLE     C-termini are essential and distinct for nucleic acid binding of 
            human NABP1 and NABP2.
  JOURNAL   Biochim Biophys Acta. 2016 Feb;1860(2):371-83. doi: 
            10.1016/j.bbagen.2015.11.003. Epub 2015 Nov 10.
  PUBMED    26550690
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bbagen.2015.11.003"; journalName: "Biochim Biophys Acta";
            date: "2016-02"; volume: "1860"; issue: "2"; pages: "371-83"
COMMENT     Sequence Label: pET29a-hNABP1NhNABP2C
FEATURES             Location/Qualifiers
     source          1..1014
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             686..703
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(756..774)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 ctnnaataat tttnnttaac tttaagaagg agatatacat atgaataggg tcaacgaccc
       61 acttattttt ataagagata ttaagcccgg actgaaaaac ttaaatgtcg tctttattgt
      121 cctggagata ggacgcgtga ccaaaaccaa agacggccat gaagtgagat cgtgcaaagt
      181 agcagataaa acgggcagca tcactatttc cgtgtgggat gagatcggag gtcttataca
      241 gccaggggat attattcggt tgaccagagg gtatgcatcc atgtggaaag gatgtctgac
      301 actttatact ggaaggggtg gtgaacttca aaaaattggg gaattttgta tggtttattc
      361 agaagtgcca aatttcagtg aacccaaccc agattatcga ggacagcaga acaaggcggt
      421 gcagaacgac agcaaccctt cagcttccca gcctaccact ggaccctctg ctgcctctcc
      481 agcctctgag aaccagaatg ggaatggact gagtgcccca ccaggtcccg gtggtggccc
      541 acatccccct catactccct cccacccacc cagcacccga atcactcgaa gccagcccaa
      601 ccacacacct gcaggcccgc ctggcccttc cagcaaccct gttagtaacg gcanagaaac
      661 ccggangagc agcaagagac tcgagcacca ccaccaccac cactgagatc cggctgctaa
      721 caaagcccga aangaagctg agttggctgc tgccaccgct gagcaataac tagcataccc
      781 cttggggcct ctaaacgggt cttgangggt tttttgctga nnggangaac tatatccgga
      841 tnggcgaatg ggacgcgccc tgnagcggcg cnttaagcgc ggcgggtngt ggtggttacg
      901 cgcagcgnga ccgctacact tgccagcgcc ctancgcccn gctccntatc gctttcttcc
      961 cttcctttct nnnccacgtt cgncngcttn ncccgtnnnn nnntaaatcc gggg
//
