LOCUS       sequence-262905-        9926 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9926)
  AUTHORS   Bertrand E, Chartrand P, Schaefer M, Shenoy SM, Singer RH, Long RM
  TITLE     Localization of ASH1 mRNA particles in living yeast.
  JOURNAL   Mol Cell. 1998 Oct . 2(4):437-45.
  PUBMED    9809065
REFERENCE   2  (bases 1 to 9926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            1998 Oct . 2(4):437-45."
COMMENT     Sequence Label: pG14-MS2-GFP
FEATURES             Location/Qualifiers
     source          1..9926
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        50..693
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     586..609
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     CDS             707..727
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             731..757
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             731..757
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             758..769
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             1148..1858
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     terminator      1979..2249
                     /gene="S. cerevisae PGK1"
                     /label=PGK1 terminator
                     /note="transcription terminator for 3-phosphoglycerate 
                     kinase gene"
     primer_bind     complement(2329..2348)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        2547..2648
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     promoter        2977..3382
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             3383..4477
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(4975..4992)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4975..4991)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4984..5006)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5200..5219)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5319..5341
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5379..5397)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5465..5569
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5570..5638,5639..6430)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5788..5807)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6601..7189
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7090..7109
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7343..7360
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7477..7498
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7513..7518,7519..7536,7537..7543)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7551..7567
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7556..7578
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7575..7591
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7575..7591
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      7943..9285
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    8275..8322
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 tcatgtttga cagcttatca tcgataagct tggctgcagg tcgagtttat cattatcaat
       61 actgccattt caaagaatac gtaaataatt aatagtagtg attttcctaa ctttatttag
      121 tcaaaaaatt agccttttaa ttctgctgta acccgtacat gcccaaaata gggggcgggt
      181 tacacagaat atataacatc gtaggtgtct gggtgaacag tttattcctg gcatccacta
      241 aatataatgg agcccgcttt ttaagctggc atccagaaaa aaaaagaatc ccagcaccaa
      301 aatattgttt tcttcaccaa ccatcagttc ataggtccat tctcttagcg caactacaga
      361 gaacaggggc acaaacaggc aaaaaacggg cacaacctca atggagtgat gcaacctgcc
      421 tggagtaaat gatgacacaa ggcaattgac ccacgcatgt atctatctca ttttcttaca
      481 ccttctatta ccttctgctc tctctgattt ggaaaaagct gaaaaaaaag gttgaaacca
      541 gttccctgaa attattcccc tacttgacta ataagtatat aaagacggta ggtattgatt
      601 gtaattctgt aaatctattt cttaaacttc ttaaattcta cttttatagt tagtcttttt
      661 tttagtttta aaacaccaag aacttagttt cgacggatcc accatgccaa aaaagaaaag
      721 aaaagttggc tacccctacg acgtgcccga ctacgccatc gaaggccgcc atatgctagc
      781 cgttaaaatg gcttctaact ttactcagtt cgttctcgtc gacaatggcg gaactggcga
      841 cgtgactgtc gccccaagca acttcgctaa cgggatcgct gaatggatca gctctaactc
      901 gcgttcacag gcttacaaag taacctgtag cgttcgtcag agctctgcgc agaatcgcaa
      961 atacaccatc aaagtcgagg tgcctaaagg cgcctggcgt tcgtacttaa atatggaact
     1021 aaccattcca attttcgcca cgaattccga ctgcgagctt attgttaagg caatgcaagg
     1081 tctcctaaaa gatggaaacc cgattccctc agcaatcgca gcaaactccg gcatctacgc
     1141 ggccgcttct aaaggtgaag aattattcac tggtgttgtc ccaattttgg ttgaattaga
     1201 tggtgatgtt aatggtcaca aattttctgt ctccggtgaa ggtgaaggtg atgctactta
     1261 cggtaaattg accttaaaat ttatttgtac tactggtaaa ttgccagttc catggccaac
     1321 cttagtcact actttcggtt atggtgttca atgttttgcg agatacccag atcatatgaa
     1381 acaacatgac tttttcaagt ctgccatgcc agaaggttat gttcaagaaa gaactatttt
     1441 tttcaaagat gacggtaact acaagaccag agctgaagtc aagtttgaag gtgatacctt
     1501 agttaataga atcgaattaa aaggtattga ttttaaagaa gatggtaaca ttttaggtca
     1561 caaattggaa tacaactata actctcacaa tgtttacatc atggctgaca aacaaaagaa
     1621 tggtatcaaa gttaacttca aaattagaca caacattgaa gatggttctg ttcaattagc
     1681 tgaccattat caacaaaata ctccaattgg tgatggtcca gtcttgttac cagacaacca
     1741 ttacttatcc actcaatctg ccttatccaa agatccaaac gaaaagagag accacatggt
     1801 cttgttagaa tttgttactg ctgctggtat tacccatggt atggatgaat tgtacaaagt
     1861 cgacctagat aagtaatgat ccgggatctc ccatgtctct actggtggtg gtgcttcttt
     1921 ggaattattg gaaggtaagg aattgccagg tgttgctttc ttatccgaaa agaaataaat
     1981 tgaattgaat tgaaatcgat agatcaattt ttttcttttc tctttcccca tcctttacgc
     2041 taaaataata gtttatttta ttttttgaat attttttatt tatatacgta tatatagact
     2101 attatttatc ttttaatgat tattaagatt tttattaaaa aaaaattcgc tcctctttta
     2161 atgcctttat gcagtttttt tttcccattc gatatttcta tgttcgggtt cagcgtattt
     2221 taagtttaat aactcgaaaa ttctgcgttc gttaaagctc cggatcgatc ctctacgccg
     2281 gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg
     2341 acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg
     2401 tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac
     2461 cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc
     2521 aggagtcgca taagggagag cgtcgaaatt cggtcgaaaa aagaaaagga gagggccaag
     2581 agggagggca ttggtgacta ttgagcacgt gagtatacgt gattaagcac acaaaggcag
     2641 cttggagtat gtctgttatt aatttcacag gtagttctgg tccattggtg aaagtttgcg
     2701 gcttgcagag cacagaggcc gcagaatgtg ctctagtcga ggagaacttc tagtatatct
     2761 acatacctaa tattattgcc ttattaaaaa tggaatccca acaattacat caaaatccac
     2821 attctcttca aaatcaattg tcctgtactt ccttgttcat gtgtgttcaa aaacgttata
     2881 tttataggat aattatactc tatttctcaa caagtaattg gttgtttggc cgagcggtct
     2941 aaggcgcctg attcaagaaa tatcttgacc gcagttaact gtgggaatac tcaggtatcg
     3001 taagatgcaa gagttcgaat ctcttagcaa ccattatttt tttcctcaac ataacgagaa
     3061 cacacagggg cgctatcgca cagaatcaaa ttcgatgact ggaaattttt tgttaatttc
     3121 agaggtcgcc tgacgcatat acctttttca actgaaaaat tgggagaaaa aggaaaggtg
     3181 agagcgccgg aaccggcttt tcatatagaa tagagaagcg ttcatgacta aatgcttgca
     3241 tcacaatact tgaagttgac aatattattt aaggacctat tgttttttcc aataggtggt
     3301 tagcaatcgt cttactttct aacttttctt accttttaca tttcagcaat atatatatat
     3361 atttcaagga tataccattc taatgtctgc ccctaagaag atcgtcgttt tgccaggtga
     3421 ccacgttggt caagaaatca cagccgaagc cattaaggtt cttaaagcta tttctgatgt
     3481 tcgttccaat gtcaagttcg atttcgaaaa tcatttaatt ggtggtgctg ctatcgatgc
     3541 tacaggtgtt ccacttccag atgaggcgct ggaagcctcc aagaaggctg atgccgtttt
     3601 gttaggtgct gtgggtggtc ctaaatgggg taccggtagt gttagacctg aacaaggttt
     3661 actaaaaatc cgtaaagaac ttcaattgta cgccaactta agaccatgta actttgcatc
     3721 cgactctctt ttagacttat ctccaatcaa gccacaattt gctaaaggta ctgacttcgt
     3781 tgttgtcaga gaattagtgg gaggtattta ctttggtaag agaaaggaag acgatggtga
     3841 tggtgtcgct tgggatagtg aacaatacac cgttccagaa gtgcaaagaa tcacaagaat
     3901 ggccgctttc atggccctac aacatgagcc accattgcct atttggtcct tggataaagc
     3961 taatgttttg gcctcttcaa gattatggag aaaaactgtg gaggaaacca tcaagaacga
     4021 attccctaca ttgaaggttc aacatcaatt gattgattct gccgccatga tcctagttaa
     4081 gaacccaacc cacctaaatg gtattataat caccagcaac atgtttggtg atatcatctc
     4141 cgatgaagcc tccgttatcc caggttcctt gggtttgttg ccatctgcgt ccttggcctc
     4201 tttgccagac aagaacaccg catttggttt gtacgaacca tgccacggtt ctgctccaga
     4261 tttgccaaag aataaggtca accctatcgc cactatcttg tctgctgcaa tgatgttgaa
     4321 attgtcattg aacttgcctg aagaaggtaa ggccattgaa gatgcagtta aaaaggtttt
     4381 ggatgcaggt atcagaactg gtgatttagg tggttccaac agtaccaccg aagtcggtga
     4441 tgctgtcgcc gaagaagtta agaaaatcct tgcttaaaaa gattctcttt ttttatgata
     4501 tttgtacata aactttataa atgaaattca taatagaaac gacacgaaat tacaaaatgg
     4561 aatatgttca tagggtagac gaaactatat acgcaatcta catacattta tcaagaagga
     4621 gaaaaaggag gatgtaaagg aatacaggta agcaaattga tactaatggc tcaacgtgat
     4681 aaggaaaaag aattgcactt taacattaat attgacaagg aggagggcac cacacaaaaa
     4741 gttaggtgta acagaaaatc atgaaactat gattcctaat ttatatattg gaggattttc
     4801 tctaaaaaaa aaaaaataca acaaataaaa aacactcaat gacctgacca tttgatggag
     4861 tttaagtcaa taccttcttg aaccatttcc cataatggtg aaagttccct caagaatttt
     4921 actctgtcag aaacggcctt aacgacgtag tcgagatctg catgcaagct tggcactggc
     4981 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     5041 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     5101 ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc tccttacgca
     5161 tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc
     5221 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     5281 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     5341 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
     5401 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     5461 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     5521 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     5581 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     5641 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     5701 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     5761 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     5821 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     5881 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     5941 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     6001 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     6061 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     6121 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     6181 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     6241 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     6301 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     6361 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     6421 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     6481 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     6541 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     6601 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     6661 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     6721 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     6781 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     6841 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     6901 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     6961 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     7021 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     7081 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     7141 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     7201 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     7261 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     7321 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     7381 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     7441 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     7501 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     7561 ataacaattt cacacaggaa acagctatga catgattacg aattctgaac cagtcctaaa
     7621 acgagtaaat aggaccggca attcttcaag caataaacag gaataccaat tattaaaaga
     7681 taacttagtc agatcgtaca ataaagcttt gaagaaaaat gcgccttatt caatctttgc
     7741 tataaaaaat ggcccaaaat ctcacattgg aagacatttg atgacctcat ttctttcaat
     7801 gaagggccta acggagttga ctaatgttgt gggaaattgg agcgataagc gtgcttctgc
     7861 cgtggccagg acaacgtata ctcatcagat aacagcaata cctgatcact acttcgcact
     7921 agtttctcgg tactatgcat atgatccaat atcaaaggaa atgatagcat tgaaggatga
     7981 gactaatcca attgaggagt ggcagcatat agaacagcta aagggtagtg ctgaaggaag
     8041 catacgatac cccgcatgga atgggataat atcacaggag gtactagact acctttcatc
     8101 ctacataaat agacgcatat aagtacgcat ttaagcataa acacgcacta tgccgttctt
     8161 ctcatgtata tatatataca ggcaacacgc agatataggt gcgacgtgaa cagtgagctg
     8221 tatgtgcgca gctcgcgttg cattttcgga agcgctcgtt ttcggaaacg ctttgaagtt
     8281 cctattccga agttcctatt ctctagaaag tataggaact tcagagcgct tttgaaaacc
     8341 aaaagcgctc tgaagacgca ctttcaaaaa accaaaaacg caccggactg taacgagcta
     8401 ctaaaatatt gcgaataccg cttccacaaa cattgctcaa aagtatctct ttgctatata
     8461 tctctgtgct atatccctat ataacctacc catccacctt tcgctccttg aacttgcatc
     8521 taaactcgac ctctacattt tttatgttta tctctagtat tactctttag acaaaaaaat
     8581 tgtagtaaga actattcata gagtgaatcg aaaacaatac gaaaatgtaa acatttccta
     8641 tacgtagtat atagagacaa aatagaagaa accgttcata attttctgac caatgaagaa
     8701 tcatcaacgc tatcactttc tgttcacaaa gtatgcgcaa tccacatcgg tatagaatat
     8761 aatcggggat gcctttatct tgaaaaaatg cacccgcagc ttcgctagta atcagtaaac
     8821 gcgggaagtg gagtcaggct ttttttatgg aagagaaaat agacaccaaa gtagccttct
     8881 tctaacctta acggacctac agtgcaaaaa gttatcaaga gactgcatta tagagcgcac
     8941 aaaggagaaa aaaagtaatc taagatgctt tgttagaaaa atagcgctct cgggatgcat
     9001 ttttgtagaa caaaaaagaa gtatagattc tttgttggta aaatagcgct ctcgcgttgc
     9061 atttctgttc tgtaaaaatg cagctcagat tctttgtttg aaaaattagc gctctcgcgt
     9121 tgcatttttg ttttacaaaa atgaagcaca gattcttcgt tggtaaaata gcgctttcgc
     9181 gttgcatttc tgttctgtaa aaatgcagct cagattcttt gtttgaaaaa ttagcgctct
     9241 cgcgttgcat ttttgttcta caaaatgaag cacagatgct tcgttaacaa agatatgcta
     9301 ttgaagtgca agatggaaac gcagaaaatg aaccggggat gcgacgtgca agattaccta
     9361 tgcaatagat gcaatagttt ctccaggaac cgaaatacat acattgtctt ccgtaaagcg
     9421 ctagactata tattattata caggttcaaa tatactatct gtttcaggga aaactcccag
     9481 gttcggatgt tcaaaattca atgatgggta acaagtacga tcgtaaatct gtaaaacagt
     9541 ttgtcggata ttaggctgta tctcctcaaa gcgtattcga atatcattga gaagctgcag
     9601 ctattcatgc aactgctaaa tggtctactg ctcaattaaa gaaaacacag gcagagcagg
     9661 ctgcccgggc aaaagctgca gcgtcacatc ggataataat gatggcagcc attgtagaag
     9721 tgccttttgc atttctagtc tctttctcgg tctagctagt tttactacat cgcgaagata
     9781 gaatcttaga tcacactgcc tttgctgagc tggatcaata gagtaacaaa agagtggtaa
     9841 ggcctcgtta aaggacaagg acctgagcgg aagtgtatcg tacagtagac ggagtatact
     9901 agtatagtct atagtccgtg gaattc
//
