LOCUS       sequence-262909-        7386 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  An AAV genome that expresses tTA from the rat TH promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7386)
  AUTHORS   Robinson JE, Coughlin GM, Hori AM, Cho JR, Mackey ED, Turan Z, 
            Patriarchi T, Tian L, Gradinaru V
  TITLE     Optical dopamine monitoring with dLight1 reveals mesolimbic 
            phenotypes in a mouse model of neurofibromatosis type 1.
  JOURNAL   Elife. 2019 Sep 23;8. pii: 48983. doi: 10.7554/eLife.48983.
  PUBMED    31545171
REFERENCE   2  (bases 1 to 7386)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2019-09-23"; volume: "8. pii: 48983. doi"; pages: " 
            10.7554/eLife.48983"
COMMENT     Sequence Label: pAAV-rTH-tTA
FEATURES             Location/Qualifiers
     source          1..7386
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     CDS             3369..3479
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     misc_feature    3513..4101
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3566..3586)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3984..3995)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4133..4609
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4332..4351)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   4649..4789
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4649..4778
                     /label=AAV2 ITR
     rep_origin      4864..5319
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4951..4970)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5161..5182
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5336..5355)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5455..5477
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5515..5533)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5601..5705
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5706..5774,5775..6566)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5924..5943)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6737..7325
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7226..7245
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtaacact agttcttggg attcttctca tcaaaaaggg
      181 catgggacac agctaaagcg tccagggctc ctctgtgccc acagatgcct tagatcttgg
      241 cacaatgtag tcagccagct ccgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt
      301 gtgtgtgttt gcatgtatct cacagacagt gcacaatggc ctggatgtga acagaggcaa
      361 gagtctgggc cagcagttgt ctcccaggag ggtccaaaga catcgtattt tcaagtttag
      421 gccaggtgct cacttgggtg agctcagaca cagacaaagg tctggagagc acacattccc
      481 cacccccacc cagcttctat gcaagcacct ccagccgaga caagaaaacg aattaaaaag
      541 caatatttgt gtcagcgtaa gacatttgcc gaaaggttaa atccacactc gtggtgctgc
      601 acagcagccc cctgtgcagg atttgttagg cacagctccc tcctaccccg tgccacctga
      661 gcaaatgcca ggctgggtgg gctggaacca gcctgggctt gcctcaccct ggaatcccca
      721 gcaccctcca aaggaggacc ctgggagtgg gcatagacgc ccttcaggtg tgggcaacag
      781 cccccagtcc tcaggatgaa aggctaaggt gcagccagct ctgccttcac ggtgggaatg
      841 tctctatgtg agccctttct gggctgtgaa gaacgctctg agaagggtcc tgggaccctg
      901 gataggccag agctgtgctg ggcatgtaga gacaggagtg ggctaaagca gcaaaggcac
      961 tgaccaagga agagttcaga gaggagcgtg gaatatgggg aggggttcat agtaagagag
     1021 agcaggcagt ggagagtaaa tagtcactga gccggggttt atggggtttg taggagctta
     1081 ctcagagaaa gtagatgaga gatgccatgc cagtctgagt atcacagagc cccaggctct
     1141 cctgggaacg gaactgtgag ggccagaagg tcagcaaggg aggttaggga gagttccttt
     1201 tgtactgact cagcatttat cctgctccca gggggcaatg ggggccagtg agggatgcag
     1261 agcaaggcag tgatgtggca ggcagttcct gttgtgaaag agctgggaag ggagcgggct
     1321 gggcctggta cgtacagcag gccatttctg agggtccgag tgctgtctag gaggtgcagt
     1381 gagacttcag tgatcagcca gaacagaagc taagcggggt ggggactgcg agttcaggct
     1441 tctgggtctt gcaaatatcc agaatgctaa atcctcagaa ccccagggtg gccattttca
     1501 gagtgggttt tgtcctttgg gcacttgtgc agactccaat atccagaggg ataaggatgg
     1561 tactcttcag tacccttagt gagaggacac ttttctctga agggcttgaa tgtgccgagc
     1621 cattacctga aggaaggaaa tgactccagg gacataggat gggcccagca caactcacct
     1681 gctacagaga aaggtcccct ccctggtctc cttagagatc ctgtttccct ggctgaggaa
     1741 gctagggtgg atctttgtgt aagtgggtgt ggatgctaac tggaaaacaa aaggtcactt
     1801 actgttagac ctcggggtac catggaagag atgatcactg agtgtgccct tacatgggga
     1861 ccagctgaga atggggctac cactagctcg agaccatgat acagggaata agtgtgcatt
     1921 tgggggtagg gagtggctca gaatactctt aaccaaagca gaggtttgct cccacaggaa
     1981 ggtgaggtca gaaggcctta gggagctgcc aggggctagg gttggcacca tctcccaggc
     2041 tgtgtcttta aggagatgat aatcagaggg atagaacctt gcaaaagtgg gccagtcttg
     2101 ggaatactat agaggaatag ccttctggaa cattctgtgt ctcataggac ctgcctgggg
     2161 atccagcccc agtgccagca catataccga ctggggcagt gaatagatag tacactttgt
     2221 tacatgggct ggggggaaca tggcccatgt cctggagggg actttatgac agacatccaa
     2281 aaatccagtg agagggcttc tagatttgtc tccaaaggtt atagttctaa catgagccct
     2341 taggaaatcc agcatggttc tccctgtgtg ccctggtttg gttagagagc tctagcggtc
     2401 tcctgtccca cagaatacca gccagcccct gccctacgtc gtgcctcggg ctgagggtga
     2461 ttcagaggca ggtgcctgtg acagtggatg caattagatc taatgggacg gaggcctttc
     2521 tcgtcgccct cgctccatgc ccacccccgc ctccctcagg cacagcaggc gtggagagga
     2581 tgcgcaggag gtaggaggtg ggggacccag aggggctttg acgtcagcct ggcctttaaa
     2641 gagggcgcct gcctggcgag ggctgtggag acagaactcg ggaccaccag gcggcccaac
     2701 ggtaccggat cccttaaggg cccggatccg gtaccgccac catgagtcgg ctggataaat
     2761 ctaaagtcat aaacggcgct ctggaattac tcaatgaagt cggtatcgaa ggcctgacga
     2821 caaggaaact cgctcaaaag ctgggagttg agcagcctac cctgtactgg cacgtgaaga
     2881 acaagcgggc cctgctcgat gccctggcca tcgagatgct ggacaggcat catacccact
     2941 tctgccccct ggaaggcgag tcatggcaag actttctgcg gaacaacgcc aagtcattcc
     3001 gctgtgctct cctctcacat cgcgacgggg ctaaagtgca tctcggcacc cgcccaacag
     3061 agaaacagta cgaaaccctg gaaaatcagc tcgcgttcct gtgtcagcaa ggcttctccc
     3121 tggagaacgc actgtacgct ctgtccgccg tgggccactt tacactgggc tgcgtattgg
     3181 aggaacagga gcatcaagta gcaaaagagg aaagagagac acctaccacc gattctatgc
     3241 ccccacttct gagacaagca attgagctgt tcgaccggca gggagccgaa cctgccttcc
     3301 ttttcggcct ggaactaatc atatgtggcc tggagaaaca gctaaagtgc gaaagcggcg
     3361 ggccggccga cgcccttgac gattttgact tagacatgct cccagccgat gcccttgacg
     3421 actttgacct tgatatgctg cctgctgacg ctcttgacga ttttgacctt gacatgctcc
     3481 ccgggtaaga attcgatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg
     3541 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     3601 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     3661 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     3721 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     3781 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     3841 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     3901 cggggaaatc atcgtccttt ccttggctgc tcgcctatgt tgccacctgg attctgcgcg
     3961 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     4021 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     4081 ccctttgggc cgcctccccg catcgatacc gagcgctgct cgagagatct acgggtggca
     4141 tccctgtgac ccctccccag tgcctctcct ggccctggaa gttgccactc cagtgcccac
     4201 cagccttgtc ctaataaaat taagttgcat cattttgtct gactaggtgt ccttctataa
     4261 tattatgggg tggagggggg tggtatggag caaggggcaa gttgggaaga caacctgtag
     4321 ggcctgcggg gtctattggg aaccaagctg gagtgcagtg gcacaatctt ggctcactgc
     4381 aatctccgcc tcctgggttc aagcgattct cctgcctcag cctcccgagt tgttgggatt
     4441 ccaggcatgc atgaccaggc tcagctaatt tttgtttttt tggtagagac ggggtttcac
     4501 catattggcc aggctggtct ccaactccta atctcaggtg atctacccac cttggcctcc
     4561 caaattgctg ggattacagg cgtgaaccac tgctcccttc cctgtccttc tgattttgta
     4621 ggtaaccacg tgcggaccga gcggccgcag gaacccctag tgatggagtt ggccactccc
     4681 tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc
     4741 tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg
     4801 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat
     4861 agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     4921 ccgctacact tgccagcgcc ttagcgcccg ctcctttcgc tttcttccct tcctttctcg
     4981 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     5041 ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg
     5101 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     5161 gtggactctt gttccaaact ggaacaacac tcaactctat ctcgggctat tcttttgatt
     5221 tataagggat tttgccgatt tcggtctatt ggttaaaaaa tgagctgatt taacaaaaat
     5281 ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa
     5341 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     5401 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     5461 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
     5521 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     5581 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     5641 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     5701 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     5761 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     5821 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     5881 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     5941 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     6001 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     6061 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     6121 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     6181 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     6241 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     6301 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     6361 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     6421 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     6481 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     6541 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     6601 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     6661 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     6721 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     6781 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     6841 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     6901 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     6961 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     7021 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     7081 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     7141 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     7201 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     7261 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     7321 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     7381 acatgt
//
