LOCUS       sequence-262921-        4638 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4638)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4638)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_XPR1
FEATURES             Location/Qualifiers
     source          1..4638
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2763..2862)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2879..2898)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2880..2898)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2903..2919)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2903..2919)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3021..3040)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3032..3841
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3099..3118)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3988..4576
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4477..4496
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatgaagt tcgccgagca cctgtctgcc cacatcaccc ctgagtggcg
      721 gaagcagtat atccagtacg aggcctttaa ggacatgctg tacagcgccc aggatcaggc
      781 accatccgtg gaggtgaccg acgaggatac agtgaagaga tatttcgcca agtttgagga
      841 gaagttcttt cagacctgcg agaaggagct ggccaagatc aacacattct actccgagaa
      901 gctggcagag gcacagcgga gatttgccac cctgcagaat gagctgcaga gctccctgga
      961 cgcccagaag gagtctacag gcgtgaccac actgcggcag aggcgcaagc ccgtgttcca
     1021 cctgagccac gaggagaggg tgcagcaccg caacatcaag gatctgaagc tggccttctc
     1081 cgagttttac ctgtctctga tcctgctgca gaattatcag aacctgaatt tcaccggctt
     1141 tagaaagatc ctgaagaagc acgacaagat cctggagaca tccaggggag cagattggag
     1201 ggtggcacac gtggaggtgg ccccattcta tacctgcaag aagatcaacc agctgatctc
     1261 tgagaccgag gccgtggtga caaatgagct ggaggacggc gataggcaga aggccatgaa
     1321 gaggctgagg gtgccacctc tgggagcagc acagccagca ccagcatgga ccacatttcg
     1381 cgtgggactg ttctgtggca tctttatcgt gctgaacatc accctggtgc tggccgccgt
     1441 gttcaagctg gagacagaca ggagcatctg gccactgatc aggatctacc gcggcggctt
     1501 tctgctgatc gagttcctgt ttctgctggg catcaacacc tatggctgga ggcaggccgg
     1561 cgtgaatcac gtgctgatct tcgagctgaa cccccgctcc aatctgtctc accagcacct
     1621 gttcgagatc gccggctttc tgggcatcct gtggtgcctg agcctgctgg cctgtttctt
     1681 tgcccctatc tccgtgatcc caacctacgt gtaccccctg gccctgtacg gcttcatggt
     1741 gttctttctg atcaacccaa ccaagacatt ctactataag agcaggtttt ggctgctgaa
     1801 gctgctgttc cgcgtgttta cagccccctt ccacaaagtg ggcttcgccg acttttggct
     1861 ggccgatcag ctgaattccc tgtctgtgat cctgatggac ctggagtata tgatctgctt
     1921 ttactctctg gagctgaagt gggatgagag caagggcctg ctgcccaaca atagcgagga
     1981 gtccggcatc tgtcacaagt atacctacgg cgtgcgggca atcgtgcagt gcatcccagc
     2041 atggctgaga ttcatccagt gtctgcggag ataccgggac acaaagagag cctttcctca
     2101 cctggtgaac gccggcaagt acagcaccac attctttatg gtgaccttcg ccgccctgta
     2161 ttccacacac aaggagagag gccactctga caccatggtg ttcttttatc tgtggatcgt
     2221 gttttacatc atctctagct gctatacact gatctgggac ctgaagatgg attggggcct
     2281 gttcgataag aacgccggcg agaatacctt tctgcgggag gagatcgtgt atcctcagaa
     2341 ggcctactat tactgtgcca tcatcgagga cgtgatcctg agattcgcct ggaccatcca
     2401 gatctctatc acaagcacca cactgctgcc tcactccggc gatatcatcg ccaccgtgtt
     2461 tgccccactg gaggtgttcc ggcggttcgt gtggaacttc tttaggctgg agaatgagca
     2521 cctgaacaat tgtggcgagt tccgggccgt gagagacatc agcgtggcac ccctgaacgc
     2581 cgacgatcag accctgctgg agcagatgat ggatcaggac gatggcgtgc ggaacagaca
     2641 gaagaatcgc tcctggaagt acaatcagag catctccctg cggagacctc ggctggcctc
     2701 tcagagcaag gccagagaca caaaggtgct gatcgaggat accgacgatg aggccaatac
     2761 agacccagct ttcttgtaca aagttggcat tataagaaag cattgcttat caatttgttg
     2821 caacgaacag gtcactatca gtcaaaataa aatcattatt tgccatccag ctgatatccc
     2881 ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca
     2941 aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc
     3001 tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag
     3061 gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa
     3121 tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt
     3181 gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact
     3241 aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga
     3301 tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga
     3361 atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca
     3421 ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc
     3481 gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg
     3541 ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc
     3601 agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat
     3661 aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct
     3721 atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg
     3781 tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta
     3841 atcagaattg gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg
     3901 cgcaagctca tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga
     3961 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     4021 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     4081 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     4141 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     4201 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     4261 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     4321 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     4381 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     4441 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     4501 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     4561 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     4621 gccttttgct cacatgtt
//
