LOCUS       sequence-262933-        3933 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3933)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 3933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC30A6
FEATURES             Location/Qualifiers
     source          1..3933
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2058..2157)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2174..2193)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2175..2193)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2198..2214)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2198..2214)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2316..2335)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2327..3136
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2394..2413)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3283..3871
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3772..3791
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatgggca ccatccacct gttcaggaag ccccagcgga gcttcttcgg
      721 caagctgctg cgggagttta gactggtggc cgccgaccgg agatcttgga agatcctgct
      781 gttcggcgtg atcaatctga tctgcacagg ctttctgctg atgtggtgta gctccaccaa
      841 cagcatcgcc ctgaccgcct acacatatct gaccatcttc gatctgttta gcctgatgac
      901 atgcctgatc tcctactggg tgaccctgag gaagccatct cccgtgtata gcttcggctt
      961 tgagcgcctg gaggtgctgg ccgtgttcgc atctaccgtg ctggcacagc tgggcgccct
     1021 gttcatcctg aaggagagcg ccgagcggtt tctggagcag ccagagatcc acacaggcag
     1081 actgctggtg ggcaccttcg tggccctgtg cttcaacctg tttacaatgc tgtctatccg
     1141 gaataagccc ttcgcctacg tgagcgaggc agcatctacc agctggctgc aggagcacgt
     1201 ggcagacctg tccaggtctc tgtgcggcat catccctggc ctgtctagca tcttcctgcc
     1261 tcgcatgaat ccatttgtgc tgatcgacct ggcaggagcc ttcgccctgt gcatcacata
     1321 catgctgatc gagatcaaca attattttgc cgtggatacc gccagcgcca tcgccatcgc
     1381 cctgatgaca ttcggcacca tgtacccaat gagcgtgtat tccggcaagg tgctgctgca
     1441 gaccacaccc cctcacgtga tcggccagct ggacaagctg atccgggagg tgtccacact
     1501 ggatggcgtg ctggaggtga gaaacgagca cttctggacc ctgggatttg gatccctggc
     1561 aggatctgtg cacgtgcgga tcaggcgcga cgccaacgag cagatggtgc tggcccacgt
     1621 gacaaataga ctgtacaccc tggtgagcac cctgacagtg cagatcttta aggacgattg
     1681 gatcaggccc gccctgctgt ccggacctgt ggcagccaac gtgctgaatt tctctgatca
     1741 ccacgtgatc cctatgccac tgctgaaggg cacagacgat ctgaatcctg tgaccagcac
     1801 accagccaag ccctcctctc caccccctga gttctccttt aacaccccag gcaagaacgt
     1861 gaatcccgtg atcctgctga acacccagac acgcccttac ggctttggcc tgaatcacgg
     1921 ccacacccca tatagctcca tgctgaacca gggactggga gtgccaggaa tcggagccac
     1981 acagggcctg aggacaggct tcaccaatat cccttcccgc tatggcacca acaatcggat
     2041 cggccagccc agacctgacc cagctttctt gtacaaagtt ggcattataa gaaagcattg
     2101 cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca
     2161 tccagctgat atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct
     2221 ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg
     2281 aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca
     2341 acgggaaacg tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa
     2401 atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc
     2461 cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga
     2521 tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt
     2581 tatccgtact cctgatgatg catggttact caccactgcg atccccggaa aaacagcatt
     2641 ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt
     2701 cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt
     2761 tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga
     2821 tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc
     2881 attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga
     2941 cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca
     3001 ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct
     3061 ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct
     3121 cgatgagttt ttctaatcag aattggttaa ttggttgtaa cactggcaga gcattacgct
     3181 gacttgacgg gacggcgcaa gctcatgacc aaaatccctt aacgtgagtt acgcgtcgtt
     3241 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     3301 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     3361 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     3421 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     3481 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     3541 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     3601 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     3661 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     3721 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     3781 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     3841 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     3901 cctggccttt tgctggcctt ttgctcacat gtt
//
