LOCUS       sequence-262934-        4086 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4086)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4086)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC2A7
FEATURES             Location/Qualifiers
     source          1..4086
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2211..2310)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2327..2346)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2328..2346)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2351..2367)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2351..2367)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2469..2488)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2480..3289
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2547..2566)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3436..4024
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3925..3944
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatggaga acaaggaggc aggaacacca cctccaatcc cttccaggga
      721 gggcagactg cagccaaccc tgctgctggc cacactgtct gccgccttcg gcagcgcctt
      781 tcagtacggc tataacctgt ctgtggtgaa taccccacac aaggtgttca agagctttta
      841 caatgagacc tatttcgaga ggcacgccac cttcatggac ggcaagctga tgctgctgct
      901 gtggtcctgc acagtgtcta tgttcccact gggaggactg ctgggatccc tgctggtggg
      961 cctgctggtg gattcttgtg gccgcaaggg caccctgctg atcaacaata tctttgccat
     1021 catccctgcc atcctgatgg gcgtgtccaa ggtggcgaaa gccttcgaac ttattgtatt
     1081 ttctagggtg gtgctgggcg tgtgcgcagg aatctcttac agcgccctgc ccatgtatct
     1141 gggcgagctg gcccctaaga acctgagagg catggtgggc accatgacag aggtgttcgt
     1201 gatcgtgggc gtgttcctgg cccagatctt tagcctgcag gcaatcctgg gaaatccagc
     1261 aggatggccc gtgctgctgg ccctgacagg cgtgcccgcc ctgctgcagc tgctgaccct
     1321 gccattcttt cccgagagcc ctcggtactc cctgatccag aagggcgacg aggccacagc
     1381 aaggcaggcc ctgcggagac tgaggggaca caccgacatg gaggcagagc tggaggatat
     1441 gagagcagag gcaagggcag agagggcaga gggacacctg tccgtgctgc acctgtgcgc
     1501 actgcggagc ctgagatggc agctgctgtc catcatcgtg ctgatggcag gacagcagct
     1561 gagcggcatc aacgccatca attactatgc cgataccatc tacacatccg ccggagtgga
     1621 ggcagcacac tctcagtatg tgacagtggg cagcggcgtg gtgaacatcg tgatgaccat
     1681 cacaagcgcc gtgctggtgg agaggctggg caggcgccac ctgctgctgg caggatacgg
     1741 aatctgcgga tctgcctgtc tggtgctgac cgtggtgctg ctgttccaga atcgcgtgcc
     1801 tgagctgagc tacctgggca tcatctgcgt gtttgcctat atcgcaggac actctatcgg
     1861 accaagccca gtgccttccg tggtgcggac agagatcttc ctgcagagct cccggagagc
     1921 cgcctttatg gtggacggcg ccgtgcactg gctgaccaac ttcatcatcg gcttcctgtt
     1981 tcctagcatc caggaggcca tcggcgccta ttccttcatc atctttgccg gcatctgtct
     2041 gctgaccgcc atctacatct atgtggtcat cccagagacc aagggcaaga cattcgtgga
     2101 gatcaacaga atctttgcca agaggaatcg cgtgaagctg ccagaggaga aggaggagac
     2161 catcgatgca ggaccaccta cagcatcccc agccaaggag acctcttttg acccagcttt
     2221 cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt
     2281 cactatcagt caaaataaaa tcattatttg ccatccagct gatatcccct atagtgagtc
     2341 gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg
     2401 ttacattgca caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa
     2461 cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa
     2521 ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc
     2581 aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca
     2641 tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac
     2701 ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt
     2761 actcaccact gcgatccccg gaaaaacagc attccaggta ttagaagaat atcctgattc
     2821 aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt
     2881 ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat
     2941 gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga
     3001 acaagtctgg aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca
     3061 tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga
     3121 tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct
     3181 cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc
     3241 tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat cagaattggt
     3301 taattggttg taacactggc agagcattac gctgacttga cgggacggcg caagctcatg
     3361 accaaaatcc cttaacgtga gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa
     3421 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     3481 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     3541 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     3601 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     3661 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     3721 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     3781 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     3841 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     3901 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     3961 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     4021 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     4081 catgtt
//
