LOCUS       sequence-262960-        6189 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for heterologous protein secretion in Lactobacillus 
            plantarum and L. sakei.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6189)
  AUTHORS   Mathiesen G, Sveen A, Brurberg MB, Fredriksen L, Axelsson L, Eijsink
            VG
  TITLE     Genome-wide analysis of signal peptide functionality in 
            Lactobacillus plantarum WCFS1.
  JOURNAL   BMC Genomics. 2009 Sep 10;10:425. doi: 10.1186/1471-2164-10-425.
  PUBMED    19744343
REFERENCE   2  (bases 1 to 6189)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Genomics."; date: "2009-09-10"; volume: "10:425. doi"; pages: " 
            10.1186/1471-2164-10-425"
COMMENT     Sequence Label: pLp_3050sNuc
FEATURES             Location/Qualifiers
     source          1..6189
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      951..1539
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1440..1459
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atgctttata aaataatata taggagtatg attcatatga aaaaatttaa ctttaaaacc
       61 atgttgctat tagttttggc tagttgtgtc ttcggggtcg tcgttaacgt gactactagt
      121 cttggaccac aaaccgcaat caccgcccag gcctccaagg tcgacccatt gtcaactaaa
      181 aaattacata aagaacctgc gactttaatt aaagcgattg atggtgatac ggttaaatta
      241 atgtacaaag gtcaaccaat gacattcaga ctattattgg ttgatacacc tgaaacaaag
      301 catcctaaaa aaggtgtaga gaaatatggt cctgaagcaa gtgcatttac gaaaaaaatg
      361 gtagaaaatg caaagaaaat tgaagtcgag tttgacaaag gtcaaagaac tgataaatat
      421 ggacgtggct tagcgtatat ttatgctgat ggaaaaatgg taaacgaagc tttagttcgt
      481 caaggcttgg ctaaagttgc ttatgtttac aaacctaaca atacacatga acaacattta
      541 agaaaaagtg aagcacaagc gaaaaaagag aaattaaata tttggagcga agacaacgct
      601 gattcaggtc aataagaatt cggtaccccg ggttcgaagg cgccaagctt caaattacag
      661 cacgtgttgc tttgattgat agccaaaaag cagcagttga taaagcaatt actgatattg
      721 ctgaaaaatt gtaatttata aataaaaatc accttttaga ggtggttttt ttatttataa
      781 attattcgtt tgatttcgct ttcgatagaa caatcaaagc gagaataagg aagataaatc
      841 ccataagggc gggagcagaa tgtccgagac taattcatga ccaaaatccc ttaacgtgag
      901 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
      961 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     1021 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     1081 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     1141 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     1201 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     1261 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     1321 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     1381 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     1441 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     1501 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     1561 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     1621 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     1681 acgaccgagc gctgcgagtc agtgagcgag aaggattatt cggctggttg agacgttaaa
     1741 atgataaagg ttgtattaat cttatattac ggttataatg tactcaactt aataaatgaa
     1801 cgcaaaaaaa agaaccctca acttagcaga gttaggattc acgacttatc agcacaacct
     1861 gataagattt tcgatagcaa gtactaccaa tacaagctat ctaacttggt actattataa
     1921 catgtaggct aagtttttca accattgata cttaaagtaa acggttgtta tcgggaatct
     1981 taacagaaac ctgatagcaa ccgttttttt gttattcaat ggttagcaac catcaaagca
     2041 actaaaggct ggaaacctgt tcttagctag taaaacctcc cgtgagtgtc gttcgtgacc
     2101 ccgcttgcag ttaacaacat aggtatgcta aaccttgtcg agatcaacgc gactaaagac
     2161 gtggctggaa gactaggaaa tgatacggac aggctaacta ttaacgcaga ttattcgggt
     2221 tgctgctaaa accaactcta ataatagtta gtgcaagggc tggttgagct taaattgtct
     2281 gataaagagt tctctcttta tactgcaaaa gaagcgcagt tattcacgat taggataact
     2341 gtttgagaga gcctaagggc ttgacccttg atggtttaag caccgctatg cgtgcgggat
     2401 ccccttagaa gcaaacttag agtgtgttga tagtgcatta tcttaaaatt ttgtataata
     2461 ggaattgaag ttaaattaga tgctaaaaat aggaattgaa gttaaattag atgctaaaaa
     2521 tttgtaatta agaaggaggg attcgtcatg ttggtattcc aaatgcgtaa tgtagataaa
     2581 acatctactg ttttgaaaca gactaaaaac agtgattacg cagataaata aatacgttag
     2641 attaattcct accagtgact aatcttatga ctttttaaac agataactaa aattacaaac
     2701 aaatcgttta acttcaggag agattacatg aacaaaaata taaaatattc tcaaaacttt
     2761 ttaacgagtg aaaaagtact caaccaaata ataaaacaat tgaatttaaa agaaaccgat
     2821 accgtttacg aaattggaac aggtaaaggg catttaacga cgaaactggc taaaataagt
     2881 aaacaggtaa cgtctattga attagacagt catctattca acttatcgtc agaaaaatta
     2941 aaactgaata ctcgtgtcac tttaattcac caagatattc tacagtttca attccctaac
     3001 aaacagaggt ataaaattgt tgggaatatt ccttacaatt taagcacaca aattattaaa
     3061 aaagtggttt ttgaaagccg tgcgtctgac atctatctga ctgttgaaga aggattctac
     3121 aagcgtacct tggatattca ccgaacacta gggttgctct tgcacactca agtctcgatt
     3181 cagcaattgc ttaagctgcc agcggaatgc tttcatccta aaccaaaagt aaacagtgtc
     3241 ttaataaaac ttacccgcca taccacagat gttccagata aatattggaa gctatataag
     3301 tactttgttt caaaatgggt caatcgagaa tatcgtcaac tgtttactaa aaatcagttt
     3361 cgtcaagcaa tgaaacacgc caaagtaaac aatttaagta ccattactta tgagcaagta
     3421 ttgtctattt ttaatagtta tctattattt aacgggagga aataattcta tgagtcgctt
     3481 ttttaaattt ggaaagttac acgttactaa agggaatgga gaccggggct tcaatagagt
     3541 tcttaacgtt aatccgaaaa aaactaacgt taatattaaa aaataagatc cgcttgtgaa
     3601 ttatgtataa tttgattaga ctaaagaata ggagaaagta tgatgatatt taaaaaactt
     3661 tctcgttaag ataggttgtt ggtgagcatg ttatatacgg atgtatcggt ttccttaatg
     3721 caaaattttg ttgctatctt attaattttt ctattatata gatatattca aagaaagata
     3781 acatttaaac ggatcatatt agatatttta atagcgatta ttttttcaat attatatctg
     3841 tttatttcag atgcgtcatt acttgtaatg gtattaatgc gattagggtg gcattttcat
     3901 caacaaaaag aaaataagat aaaaacgact gatacagcta atttaattct aattatcgtg
     3961 atccagttat tgttagttgc ggttgggact attattagtc agtttaccat atcgattatc
     4021 aaaagtgatt tcagccaaaa tatattgaac aatagtgcaa cagatataac tttattaggt
     4081 attttctttg ctgttttatt tgacggcttg ttctttatat tattgaagaa taagcggact
     4141 gaattacaac atttaaatca agaaatcatt gaattttcgt tagaaaaaca atattttata
     4201 tttatattta ttttatttat agtaatagaa attattttag cagttgggaa tcttcaagga
     4261 gtaacagcca cgatattatt aaccattatc attatttttt gtgtccttat cgggatgact
     4321 ttttggcaag tgatgctttt tttgaaggct tattcgattc gccaagaagc caatgaccaa
     4381 ttggtccgga atcaacaact tcaagattat ctagtcaata tcgaacagca gtacaccgaa
     4441 ttacggcgat ttaagcatga ttatcaaaac atcttattat cgttggagag ttttgccgaa
     4501 aagggcgatc agcaacagtt taaggcgtat taccaagaat tattagcaca acggccaatt
     4561 caaagtgaaa tccaaggggc agtcattgca caactcgact acttgaaaaa tgatcctatt
     4621 cgaggattag tcattcaaaa gtttttggca gccaaacagg ctggtgttac tttaaaattc
     4681 gaaatgaccg aaccaatcga attagcaacc gctaatctat taacggttat tcggattatc
     4741 ggtattttat tagacaatgc gattgaacaa gccgttcaag aaaccgatca attggtgagt
     4801 tgtgctttct tacaatctga tggtttaatc gaaattacga ttgaaaatac ggccagtcaa
     4861 gttaagaatc tccaagcatt ttcagagtta ggctattcaa cgaaaggcgc tggtcggggg
     4921 actggtttag ctaatgtgca ggatttgatt gccaaacaaa ccaatttatt cttagaaaca
     4981 cagattgaaa atagaaagtt acgacagaca ttgatgatta cggaggaaac ttaatttgta
     5041 tcccgtttat ttattagagg atgatttaca gcaacaagcg atttatcagc aaattatcgc
     5101 gaatacgatt atgattaacg aatttgcaat gactttaaca tgcgctgcca gtgatactga
     5161 gacattgttg gcggcaatta aggatcagca acgaggttta ttctttttgg atatggaaat
     5221 tgaggataac cgccaagccg gtttagaagt ggcaactaag attcggcaga tgatgccgtt
     5281 tgcgcaaatt gtcttcatta caacccacga ggaactgaca ttattaacgt tagaacgaaa
     5341 aatagcgcct ttagattaca ttctcaagga ccaaacaatg gctgaaatca aaaggcaatt
     5401 gattgatgat ctattgttag ctgagaagca aaacgaggcg gcagcgtatc accgagaaaa
     5461 tttatttagt tataaaatag gtcctcgctt tttctcatta ccattaaagg aagttgttta
     5521 tttatatact gaaaaagaaa atccgggtca tattaatttg ttagccgtta ccagaaaggt
     5581 tacttttcca ggaaatttaa atgcgctgga agcccaatat ccaatgctct ttcggtgtga
     5641 taaaagttac ttagttaacc tatctaatat tgccaattat gacagtaaaa cacggagttt
     5701 aaaatttgta gatggcagtg aggcaaaagt ctcgttccgg aaatcacggg aactagtggc
     5761 caaattaaaa caaatgatgt agcgcctgca gcacgccaaa tgatcccagt aaaaagccac
     5821 ccgcatggcg ggtggctttt tattagccct agaagggctt cccacacgca tttcagcgcc
     5881 ttagtgcctt agtttgtgaa tcataggtgg tatagtcccg aaatacccgt ctaaggaatt
     5941 gtcagatagg cctaatgact ggcttttata atatgagata atgccgactg tactttttac
     6001 agtcggtttt ctaatgtcac taacctgccc cgttagttga agaaggtttt tatattacag
     6061 ctccagatct accggtgggc ccatattaac gtttaaccga taaagttgaa cgttaatatt
     6121 ttttttgcgc agaaatggta aattgaagca taatagtctt gtaaggtatt tagctggctg
     6181 gcgtaaagt
//
