LOCUS       sequence-262968-        4623 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4623)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4623)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC28A3
FEATURES             Location/Qualifiers
     source          1..4623
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2748..2847)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2864..2883)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2865..2883)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2888..2904)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2888..2904)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3006..3025)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3017..3826
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3084..3103)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3973..4561
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4462..4481
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatggagc tgcggagcac cgcagcacct agagcagagg gctactccaa
      721 cgtgggcttc cagaacgagg agaattttct ggagaacgag aatacatccg gcaacaattc
      781 catccggtct agagccgtgc agtctaggga gcacaccaac acaaagcagg atgaggagca
      841 ggtgaccgtg gagcaggact ccccaaggaa tcgcgagcac atggaggacg atgacgagga
      901 gatgcagcag aagggctgcc tggagcggag atacgataca gtgtgcggct tctgtaggaa
      961 gcacaagacc acactgcgcc acatcatctg gggcatcctg ctggccggct acctggtcat
     1021 ggtcatcagc gcctgcgtgc tgaacttcca ccgggccctg cctctgtttg tgatcaccgt
     1081 ggccgccatc ttctttgtgg tgtgggatca cctgatggcc aagtacgagc acagaatcga
     1141 cgagatgctg tctccaggca ggcgcctgct gaatagccac tggttctggc tgaagtgggt
     1201 catctggagc tccctggtgc tggccgtgat cttctggttg gctttcgata cagctaagct
     1261 tggacagcag cagctggtgt cttttggcgg cctgatcatg tatatcgtgc tgctgttcct
     1321 gtttagcaag taccccacca gggtgtattg gaggcctgtg ctgtggggaa tcggactgca
     1381 gttcctgctg ggactgctga tcctgcggac agatcccggc ttcatcgcct ttgactggct
     1441 gggcagacag gtgcagacct tcctggagta cacagatgcc ggcgcctcct tcgtgtttgg
     1501 cgagaagtat aaggaccact tctttgcctt taaggtgctg cctatcgtgg tgttcttttc
     1561 caccgtgatg tctatgctgt actatctggg cctgatgcag tggatcatca ggaaagtggg
     1621 ctggatcatg ctggtgacca caggctctag cccaatcgag agcgtggtgg catccggaaa
     1681 catctttgtg ggacagaccg agagccctct gctggtgcgc ccatacctgc cttatatcac
     1741 aaagtccgag ctgcacgcca tcatgaccgc cggcttctcc acaatcgccg gctctgtgct
     1801 gggcgcctac atctcttttg gcgtgccatc ctctcacctg ctgaccgcca gcgtgatgtc
     1861 cgccccagca agcctggcag cagcaaagct gttctggcca gagaccgaga agcccaagat
     1921 cacactgaag aacgccatga agatggagtc tggcgatagc ggcaatctgc tggaggcagc
     1981 aacacaggga gcaagctcct ctatcagcct ggtggccaac atcgccgtga atctgatcgc
     2041 cttcctggcc ctgctgtcct ttatgaactc cgccctgtct tggttcggca atatgtttga
     2101 ttaccctcag ctgagcttcg agctgatctg ctcctacatc tttatgccat tctcttttat
     2161 gatgggcgtg gagtggcagg acagcttcat ggtggcccgg ctgatcggct acaagacctt
     2221 ctttaacgag tttgtggcct atgagcacct gagcaagtgg atccacctga ggaaggaggg
     2281 cggcccaaag ttcgtgaacg gcgtgcagca gtacatcagc atccgctccg agatcatcgc
     2341 cacctatgcc ctgtgcggct ttgccaatat cggatccctg ggaatcgtga tcggaggact
     2401 gacatctatg gcaccaagcc ggaagagaga tatcgcaagc ggagccgtga gagccctgat
     2461 cgcaggaacc gtggcatgct tcatgacagc ctgtatcgcc ggcatcctga gctccacccc
     2521 agtggacatc aactgtcacc acgtgctgga gaacgccttc aattccacat ttcccggcaa
     2581 taccacaaaa gtgatcgcct gctgtcagtc tctgctgtct agcaccgtgg ccaagggccc
     2641 tggcgaagtg atcccaggcg gcaatcactc tctgtatagc ctgaagggct gctgtaccct
     2701 gctgaacccc tccacattca actgtaatgg catctctaat acatttgacc cagctttctt
     2761 gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac
     2821 tatcagtcaa aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta
     2881 ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta
     2941 cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag
     3001 taatacaagg ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc
     3061 caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg
     3121 tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg
     3181 caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga
     3241 atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact
     3301 caccactgcg atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg
     3361 tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg
     3421 taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa
     3481 taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca
     3541 agtctggaaa gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg
     3601 tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt
     3661 tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg
     3721 tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga
     3781 tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa
     3841 ttggttgtaa cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc
     3901 aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat
     3961 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     4021 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     4081 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     4141 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     4201 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     4261 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     4321 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     4381 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     4441 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     4501 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     4561 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     4621 gtt
//
