LOCUS       sequence-262970-        4518 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4518)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4518)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC26A7
FEATURES             Location/Qualifiers
     source          1..4518
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2643..2742)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2759..2778)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2760..2778)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2783..2799)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2783..2799)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2901..2920)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2912..3721
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2979..2998)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3868..4456
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4357..4376
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatgaccg gcgccaagcg gaagaagaag agcatgctgt ggtccaagat
      721 gcacacacca cagtgcgagg acatcatcca gtggtgtcgg agaaggctgc ccatcctgga
      781 ttgggcccct cactacaatc tgaaggagaa cctgctgccc gacaccgtgt ctggcatcat
      841 gctggcagtg cagcaggtga cacagggact ggccttcgcc gtgctgagct ccgtgcaccc
      901 cgtgttcggc ctgtatggca gcctgttccc agccatcatc tacgccatct ttggaatggg
      961 acaccacgtg gcaaccggca cattcgccct gacctccctg atctctgcca atgccgtgga
     1021 gcgcatcgtg ccccagaata tgcagaacct gaccacacag agcaacacat ccgtgctggg
     1081 cctgtccgat tttgagatgc agaggatcca cgtggcagca gccgtgtctt tcctgggagg
     1141 cgtgatccag gtggccatgt ttgtgctgca gctgggctct gccaccttcg tggtgacaga
     1201 gcctgtgatc agcgccatga ccacaggagc agcaacccac gtggtgacaa gccaggtgaa
     1261 gtatctgctg ggcatgaaga tgccttacat ctccggccca ctgggcttct tttacatcta
     1321 tgcctacgtg tttgagaata tcaagagcgt gagactggag gccctgctgc tgtctctgct
     1381 gagcatcgtg gtgctggtgc tggtgaagga gctgaacgag cagtttaaga ggaagatcaa
     1441 ggtggtgctg cctgtggacc tggtgctgat catcgccgcc tccttcgcct gctattgtac
     1501 caatatggag aacacatacg gcctggaggt ggtgggccac atcccacagg gaatcccatc
     1561 cccaagggca ccccctatga atatcctgtc tgccgtgatc accgaggcct tcggcgtggc
     1621 cctggtggga tatgtggcat ccctggccct ggcacaggga tctgccaaga agtttaagta
     1681 cagcatcgac gataatcagg agttcctggc ccacggcctg agcaacatcg tgtctagctt
     1741 ctttttctgc atcccttccg ccgcagcaat gggaagaacc gcaggactgt actctaccgg
     1801 cgccaagaca caggtggcct gcctgatcag ctgtatcttt gtgctgatcg tgatctatgc
     1861 catcggccca ctgctgtact ggctgcccat gtgcgtgctg gcctccatca tcgtggtggg
     1921 cctgaagggc atgctgatcc agttcaggga tctgaagaag tattggaatg tggacaagat
     1981 cgattggggc atctgggtgt ctacctacgt gtttacaatc tgcttcgccg ccaacgtggg
     2041 cctgctgttt ggcgtggtgt gcaccatcgc aatcgtgatc ggccgcttcc caagggcaat
     2101 gacagtgagc atcaagaaca tgaaggagat ggagtttaag gtgaagaccg agatggacag
     2161 cgagacactg cagcaggtga agatcatctc catcaacaat cccctggtgt ttctgaatgc
     2221 caagaagttc tataccgatc tgatgaacat gatccagaag gagaatgcct gcaaccagcc
     2281 tctggacgat atcagcaagt gtgagcagaa tacactgctg aactccctgt ctaacggcaa
     2341 ttgcaacgag gaggccagcc agtcctgccc aaatgagaag tgttacctga tcctggactg
     2401 ttccggcttc acctttttcg attattccgg cgtgtctatg ctggtggagg tgtacatgga
     2461 ctgcaagggc agatccgtgg atgtgctgct ggcccactgt accgcctctc tgatcaaggc
     2521 catgacatac tatggcaacc tggactccga gaagcccatc tttttcgagt ctgtgagcgc
     2581 cgccatctct cacatccaca gcaataagaa cctgagcaag ctgtccgatc actctgaggt
     2641 ggacccagct ttcttgtaca aagttggcat tataagaaag cattgcttat caatttgttg
     2701 caacgaacag gtcactatca gtcaaaataa aatcattatt tgccatccag ctgatatccc
     2761 ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca
     2821 aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc
     2881 tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag
     2941 gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa
     3001 tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt
     3061 gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact
     3121 aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga
     3181 tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga
     3241 atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca
     3301 ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc
     3361 gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg
     3421 ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc
     3481 agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat
     3541 aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct
     3601 atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg
     3661 tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta
     3721 atcagaattg gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg
     3781 cgcaagctca tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga
     3841 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     3901 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     3961 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     4021 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     4081 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     4141 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     4201 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     4261 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     4321 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     4381 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     4441 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     4501 gccttttgct cacatgtt
//
