LOCUS       sequence-262971-        4494 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4494)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4494)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC2A13
FEATURES             Location/Qualifiers
     source          1..4494
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2619..2718)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2735..2754)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2736..2754)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2759..2775)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2759..2775)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2877..2896)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2888..3697
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2955..2974)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3844..4432
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4333..4352
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatgtcta gaaaggccag cgagaacgtg gagtacaccc tgaggagcct
      721 gagctccctg atgggcgagc ggagaaggaa gcagcctgag ccagacgcag caagcgccgc
      781 aggcgagtgt tccctgctgg cagcagcaga gtctagcacc agcctgcagt ccgccggagc
      841 aggaggagga ggagtgggcg acctggagcg cgccgcccgc cggcagttcc agcaggatga
      901 gacccctgcc ttcgtgtacg tggtggccgt gttttccgcc ctgggaggct tcctgtttgg
      961 ctatgacaca ggcgtggtgt ctggcgccat gctgctgctg aagcggcagc tgagcctgga
     1021 tgccctgtgg caggagctgc tggtgtcctc taccgtggga gcagcagccg tgtctgccct
     1081 ggcaggcggc gccctgaatg gcgtgttcgg cagaagggca gcaatcctgc tggcatctgc
     1141 cctgtttacc gcaggaagcg ccgtgctggc agcagcaaac aataaggaga cactgctggc
     1201 aggcagactg gtggtgggac tgggaatcgg aatcgcatcc atgaccgtgc ccgtgtacat
     1261 cgccgaggtg tctcccccta acctgagagg caggctggtg accatcaata cactgttcat
     1321 cacaggcggc cagttctttg cctccgtggt ggacggcgcc ttttcttacc tgcagaagga
     1381 tggctggagg tatatgctgg gactggcagc agtgcctgcc gtgatccagt tctttggctt
     1441 cctgtttctg cctgagagcc caagatggct gatccagaag ggacagaccc agaaggcaag
     1501 gaggatcctg agccagatga ggggcaacca gacaatcgac gaggagtacg attccatcaa
     1561 gaacaatatc gaggaggagg agaaggaagt gggatccgcc ggaccagtga tctgcagaat
     1621 gctgtcttat ccacccacca gaagggccct gatcgtgggc tgtggcctgc agatgttcca
     1681 gcagctgtcc ggcatcaaca ccatcatgta ctatagcgcc acaatcctgc agatgtccgg
     1741 cgtggaggac gataggctgg ccatctggct ggcctctgtg accgccttca caaatttcat
     1801 ctttaccctg gtgggcgtgt ggctggtgga gaaagtgggc cggagaaagc tgacatttgg
     1861 cagcctggca ggaaccacag tggccctgat catcctggcc ctgggattcg tgctgtccgc
     1921 ccaggtgtct ccccgcatca cctttaagcc aatcgcacct agcggacaga acgcaacctg
     1981 cacacggtac tcctattgca atgagtgtat gctggaccct gattgcggct tctgttacaa
     2041 gatgaacaag tctaccgtga tcgacagctc ctgcgtgcca gtgaacaagg caagcacaaa
     2101 tgaggcagca tggggccgct gtgagaacga gaccaagttt aagacagagg atatcttctg
     2161 ggcctacaat ttttgcccca ccccttattc ctggacagcc ctgctgggcc tgatcctgta
     2221 cctggtgttc tttgcaccag gaatgggacc aatgccatgg accgtgaact ctgagatcta
     2281 tccactgtgg gcacggagca caggaaatgc atgttctagc ggcatcaact ggatcttcaa
     2341 tgtgctggtg agcctgacct ttctgcacac agccgagtac ctgacctact atggcgcctt
     2401 ctttctgtat gcaggattcg cagcagtggg actgctgttt atctacggct gcctgcccga
     2461 gacaaagggc aagaagctgg aggagatcga gtccctgttc gacaatagac tgtgcacctg
     2521 tggcacatcc gactctgatg agggccgcta catcgagtat atccgggtga agggcagcaa
     2581 ctatcacctg tccgacaatg atgcctctga tgtggaggac ccagctttct tgtacaaagt
     2641 tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca
     2701 aaataaaatc attatttgcc atccagctga tatcccctat agtgagtcgt attacatggt
     2761 catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt acattgcaca
     2821 agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag
     2881 gggtgttatg agccatattc aacgggaaac gtcgaggccg cgattaaatt ccaacatgga
     2941 tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat
     3001 ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag
     3061 cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc
     3121 tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc
     3181 gatccccgga aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat
     3241 tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc
     3301 ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt
     3361 ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa
     3421 agaaatgcat aaacttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc
     3481 acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt
     3541 cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc
     3601 tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa
     3661 attgcagttt catttgatgc tcgatgagtt tttctaatca gaattggtta attggttgta
     3721 acactggcag agcattacgc tgacttgacg ggacggcgca agctcatgac caaaatccct
     3781 taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     3841 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     3901 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     3961 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     4021 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     4081 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     4141 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     4201 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     4261 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     4321 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     4381 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     4441 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgtt
//
