LOCUS       sequence-26302-e         792 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Boehm JS, Zhao JJ, Yao J, Kim SY, Firestein R, Dunn IF, Sjostrom SK,
            Garraway LA, Weremowicz S, Richardson AL, Greulich H, Stewart CJ, 
            Mulvey LA, Shen RR, Ambrogio L, Hirozane-Kishikawa T, Hill DE, Vidal
            M, Meyerson M, Grenier JK, Hinkle G, Root DE, Roberts TM, Lander ES,
            Polyak K, Hahn WC
  TITLE     Integrative Genomic Approaches Identify IKBKE as a Breast Cancer 
            Oncogene (Kinase Library Clones)
  JOURNAL   Cell. 2007 Jun 15. 129(6):1065-1079.
  PUBMED    17574021
REFERENCE   2  (bases 1 to 792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Jun 15. 129(6):1065-1079."
COMMENT     Sequence Label: pWZL Neo Myr Flag DYRK4
FEATURES             Location/Qualifiers
     source          1..792
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      36..45
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             42..83
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             108..131
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    138..162
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 actccttctc taggcgccgg ccggatccag aattcgccac catggggtct tcaaaatcta
       61 aaccaaagga ccccagccag cgccggcgca ggatccgagg ttaccttgac tacaaagacg
      121 atgacgacaa gcaattgaca agtttgtaca aaaaagttgg catgccggcc tcagagctca
      181 gggcttcaga aatacctttc caccctagca ttaaaaccca ggatcccaag gcagaggaga
      241 agtcaccaaa gaagcaaaag gtgactctga cagcggcaga ggccctaaag ctttttaaga
      301 accagctgtc tccatatgaa caaagtgaaa tcctgggcta cgcggagctg tggttcctgg
      361 gtcttgaagc caagaagctc gacacggctc ctgagaaatt tagcaagacg agttttgatg
      421 atgagcatgg cttctatctg aaggttctgc atgatcacat tgcctaccgc tatgaagttc
      481 tggagacaat cgggaagggg tcctttggac aggtggccaa gtgcttggat cacaaaaaca
      541 atgagctggt ggccctgaaa atcatcagga acaagaagag gtttcaccag caggccctga
      601 tggagctgaa gatcctggaa gctctcagaa agaaggacaa agacaacacc tacaatgtgg
      661 tgcatatgaa ggactttttc tactttcgca atcacttctg catcaccttt gagctcctgg
      721 gaatcaactt gtatgagttg atgaagaata acaactttca aggcttcagt ctgtccatag
      781 ttcggcgctt ca
//
