LOCUS       sequence-263083-        9560 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  R615A UPF1 inserted with GFP tagged on the N-terminus used to stably
            integrate into cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9560)
  AUTHORS   Fischer JW, Busa VF, Shao Y, Leung AKL
  TITLE     Structure-Mediated RNA Decay by UPF1 and G3BP1.
  JOURNAL   Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi: 
            10.1016/j.molcel.2020.01.021. Epub 2020 Feb 3.
  PUBMED    32017897
REFERENCE   2  (bases 1 to 9560)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2020.01.021"; journalName: "Mol Cell"; date: 
            "2020-04-2- 2"; volume: "78"; issue: "1"; pages: "70-84.e6"
COMMENT     Sequence Label: pci-EGFP-UPF1-R615A
FEATURES             Location/Qualifiers
     source          1..9560
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     680..700
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     726..750
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          890..1022
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1005..1024
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1067..1085
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1101..1110
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1107..1109,1110..1112,1113..1823)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1107..1109,1110..1112,1113..1823)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1152..1173)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1413..1432)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1760..1781
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    5262..5383
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5273..5292)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5327..5346
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      5569..6024
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5656..5675)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5866..5887
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6143..6500
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6143..6334
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6166..6186)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6351..6486
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6551..7345
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6605..6624)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7215..7234
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7409..7457
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(7498..7517)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7617..7639
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7677..7695)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7763..7867
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7868..7936,7937..8728)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8086..8105)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8899..9487
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9388..9407
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg
      661 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      721 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac
      781 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt
      841 gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa
      901 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact
      961 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac
     1021 aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact
     1081 ataggctagc gctaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg
     1141 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     1201 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
     1261 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
     1321 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     1381 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     1441 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     1501 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
     1561 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
     1621 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     1681 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc
     1741 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     1801 tcggcatgga cgagctgtac aagcttagcg tggaggcgta cgggcccagc tcgcagactc
     1861 tcactttcct ggacacggag gaggccgagc tgcttggcgc cgacacacag ggctccgagt
     1921 tcgagttcac cgactttact cttcctagcc agacgcagac gccccccggc ggccccggcg
     1981 gcccgggcgg tggcggcgcg ggaggcccgg gcggcgcggg cgcgggcgct gcggcgggac
     2041 agctcgacgc gcaggttggg cccgaaggca tcctgcagaa cggggctgtg gacgacagtg
     2101 tagccaagac cagccagttg ttggctgagt tgaacttcga ggaagatgaa gaagacacct
     2161 attacacgaa ggacctcccc atacacgcct gcagttactg tggaatacac gatcctgcct
     2221 gcgtggttta ctgtaatacc agcaagaagt ggttctgcaa cggacgtgga aatacttctg
     2281 gcagccacat tgtaaatcac cttgtgaggg caaaatgcaa agaggtgacc ctgcacaagg
     2341 acgggcccct gggggagaca gtcctggagt gctacaactg cggctgtcgc aacgtcttcc
     2401 tcctcggctt catcccggcc aaagctgact cagtggtggt gctgctgtgc aggcagccct
     2461 gtgccagcca gagcagcctc aaggacatca actgggacag ctcgcagtgg cagccgctga
     2521 tccaggaccg ctgcttcctg tcctggctgg tcaagatccc ctccgagcag gagcagctgc
     2581 gggcacgcca gatcacggca cagcagatca acaagctgga ggagctgtgg aaggaaaacc
     2641 cttctgccac gctggaggac ctggagaagc cgggggtgga cgaggagccg cagcatgtcc
     2701 tcctgcggta cgaggacgcc taccagtacc agaacatatt cgggcccctg gtcaagctgg
     2761 aggccgacta cgacaagaag ctgaaggagt cccagactca agataacatc actgtcaggt
     2821 gggacctggg ccttaacaag aagagaatcg cctacttcac tttgcccaag actgactctg
     2881 acatgcggct catgcagggg gatgagatat gcctgcggta caaaggggac cttgcgcccc
     2941 tgtggaaagg gatcggccac gtcatcaagg tccctgataa ttatggcgat gagatcgcca
     3001 ttgagctgcg gagcagcgtg ggtgcacctg tggaggtgac tcacaacttc caggtggatt
     3061 ttgtgtggaa gtcgacctcc tttgacagga tgcagagcgc attgaaaacg tttgccgtgg
     3121 atgagacctc ggtgtctggc tacatctacc acaagctgtt gggccacgag gtggaggacg
     3181 taatcatcaa gtgccagctg cccaagcgct tcacggcgca gggcctcccc gacctcaacc
     3241 actcccaggt ttatgccgtg aagactgtgc tgcaaagacc actgagcctg atccagggcc
     3301 cgccaggcac ggggaagacg gtgacgtcgg ccaccatcgt ctaccacctg gcccggcaag
     3361 gcaacgggcc ggtgctggtg tgtgctccga gcaacatcgc cgtggaccag ctaacggaga
     3421 agatccacca gacggggcta aaggtcgtgc gcctctgcgc caagagccgt gaggccatcg
     3481 actccccggt gtcttttctg gccctgcaca accagatcag gaacatggac agcatgcctg
     3541 agctgcagaa gctgcagcag ctgaaagacg agactgggga gctgtcgtct gccgacgaga
     3601 agcggtaccg ggccttgaag cgcaccgcag agagagagct gctgatgaac gcagatgtca
     3661 tctgctgcac atgtgtgggc gccggtgacc cgaggctggc caagatgcag ttccgctcca
     3721 ttttaatcga cgaaagcacc caggccaccg agccggagtg catggttccc gtggtcctcg
     3781 gggccaagca gctgatcctt gtaggcgacc actgccagct gggcccagtg gtgatgtgca
     3841 agaaggcggc caaggccggg ctgtcacagt cgctcttcga gcgcctggtg gtgctgggca
     3901 tccggcccat ccgcctgcag gtccagtacc ggatgcaccc tgcactcagc gccttcccat
     3961 ccaacatctt ctacgagggc tccctccaga atggtgtcac tgcagcggat cgtgtgaaga
     4021 agggatttga cttccagtgg ccccaacccg ataaaccgat gttcttctac gtgacccagg
     4081 gccaagagga gattgccagc tcgggcacct cctacctgaa caggaccgag gctgcgaacg
     4141 tggagaagat caccacgaag ttgctgaagg caggcgccaa gccggaccag attggcatca
     4201 tcacgcccta cgagggccag cgctcctacc tggtgcagta catgcagttc agcggctccc
     4261 tgcacaccaa gctctaccag gaggtggaga tcgccagtgt ggacgccttt cagggacgcg
     4321 agaaggactt catcatcctg tcctgtgtgc gggccaacga gcaccaaggc attggctttt
     4381 taaatgaccc caggcgtctg aacgtggccc tgaccagagc aaggtatggc gtcatcattg
     4441 tgggcaaccc gaaggcacta tcaaagcagc cgctctggaa ccacctgctg aactactata
     4501 aggagcagaa ggtgctggtg gaggggccgc tcaacaacct gcgtgagagc ctcatgcagt
     4561 tcagcaagcc acggaagctg gtcaacacta tcaacccggg agcccgcttc atgaccacag
     4621 ccatgtatga tgcccgggag gccatcatcc caggctccgt ctatgatcgg agcagccagg
     4681 gccggccttc cagcatgtac ttccagaccc atgaccagat tggcatgatc agtgccggcc
     4741 ctagccacgt ggctgccatg aacattccca tccccttcaa cctggtcatg ccacccatgc
     4801 caccgcctgg ctattttgga caagccaacg ggcctgctgc agggcgaggc accccgaaag
     4861 gcaagactgg tcgtggggga cgccagaaga accgctttgg gcttcctgga cccagccaga
     4921 ctaacctccc caacagccaa gccagccagg atgtggcgtc acagcccttc tctcagggcg
     4981 ccctgacgca gggctacatc tccatgagcc agccttccca gatgagccag cccggcctct
     5041 cccagccgga gctgtcccag gacagttacc ttggtgacga gtttaaatca caaatcgacg
     5101 tggcgctctc acaggactcc acgtaccagg gagagcgggc ttaccagcat ggcggggtga
     5161 cggggctgtc ccagtattaa aaggtggcgg cggaagagct aaagagtcga cccgggcggc
     5221 cgcttccctt tagtgagggt taatgcttcg agcagacatg ataagataca ttgatgagtt
     5281 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     5341 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
     5401 tcattttatg tttcaggttc agggggagat gtgggaggtt ttttaaagca agtaaaacct
     5461 ctacaaatgt ggtaaaatcc gataaggatc gatccgggct ggcgtaatag cgaagaggcc
     5521 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggac gcgccctgta
     5581 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     5641 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     5701 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttaga gctttacggc
     5761 acctcgaccg caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat
     5821 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     5881 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     5941 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aatatttaac gcgaatttta
     6001 acaaaatatt aacgtttaca atttcgcctg atgcggtatt ttctccttac gcatctgtgc
     6061 ggtatttcac accgcatacg cggatctgcg cagcaccatg gcctgaaata acctctgaaa
     6121 gaggaacttg gttaggtacc ttctgaggcg gaaagaacca gctgtggaat gtgtgtcagt
     6181 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     6241 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     6301 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     6361 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     6421 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     6481 gaggcctagg cttttgcaaa aagcttgatt cttctgacac aacagtctcg aacttaaggc
     6541 tagagccacc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     6601 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     6661 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     6721 gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     6781 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     6841 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     6901 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     6961 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     7021 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg
     7081 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
     7141 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     7201 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     7261 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     7321 tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg
     7381 acgcccaacc tgccatcacg atggccgcaa taaaatatct ttattttcat tacatctgtg
     7441 tgttggtttt ttgtgtgaat cgatagcgat aaggatccgc gtatggtgca ctctcagtac
     7501 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
     7561 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     7621 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct
     7681 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     7741 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     7801 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     7861 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     7921 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     7981 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     8041 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     8101 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     8161 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     8221 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     8281 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     8341 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     8401 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     8461 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     8521 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     8581 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     8641 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     8701 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     8761 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     8821 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     8881 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     8941 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     9001 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     9061 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     9121 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     9181 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     9241 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     9301 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     9361 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     9421 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     9481 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     9541 tcacatggct cgacagatct
//
