LOCUS       sequence-263094-        7152 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pFA carrying the badabA2 (locus_tag: BAS2958) and badabB2 genes 
            (locus_tag: BAS2959).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7152)
  AUTHORS   Desmarais JJ, Flamholz AI, Blikstad C, Dugan EJ, Laughlin TG, 
            Oltrogge LM, Chen AW, Wetmore K, Diamond S, Wang JY, Savage DF
  TITLE     DABs are inorganic carbon pumps found throughout prokaryotic phyla.
  JOURNAL   Nat Microbiol. 2019 Aug 12. pii: 10.1038/s41564-019-0520-8. doi: 
            10.1038/s41564-019-0520-8.
  PUBMED    31406332
REFERENCE   2  (bases 1 to 7152)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Microbiol. 2019 Aug 12. pii: 10.1038/s41564-019-0520-8. doi: 
            10.1038/s41564-019-0520-8."
COMMENT     Sequence Label: pFA-dabAB2
FEATURES             Location/Qualifiers
     source          1..7152
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(93..110)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(227..772)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(307..326)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(886..980)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1001..1660)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1575..1594
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(1661..1763)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     1829..1847
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(1868..1886,1887..1892,1893..1909,1910..1915,
                     1916..1941)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    1868..1886
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1893..1911
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             1951..1962
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      6212..6298
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(6471..7094)
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     7002..7021
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     promoter        complement(join(7095..7152,1..47))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgttt ccctcgacaa
       61 ttcgcgctag acctagggat atattccgct tcctcgctca ctgactcgct acgctcggtc
      121 gttcgactgc ggcgagcgga aatggcttac gaacggggcg gagatttcct ggaagatgcc
      181 aggaagatac ttaacaggga agtgagaggg ccgcggcaaa gccgtttttc cataggctcc
      241 gcccccctga caagcatcac gaaatctgac gctcaaatca gtggtggcga aacccgacag
      301 gactataaag ataccaggcg tttccccctg gcggctccct cgtgcgctct cctgttcctg
      361 cctttcggtt taccggtgtc attccgctgt tatggccgcg tttgtctcat tccacgcctg
      421 acactcagtt ccgggtaggc agttcgctcc aagctggact gtatgcacga accccccgtt
      481 cagtccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggaaagacat
      541 gcaaaagcac cactggcagc agccactggt aattgattta gaggagttag tcttgaagtc
      601 atgcgccggt taaggctaaa ctgaaaggac aagttttggt gactgcgctc ctccaagcca
      661 gttacctcgg ttcaaagagt tggtagctca gagaaccttc gaaaaaccgc cctgcaaggc
      721 ggttttttcg ttttcagagc aagagattac gcgcagacca aaacgatctc aagaagatca
      781 tcttattaat cagataaaat atttctagat ttcagtgcaa tttatctctt caaatgtagc
      841 acctgaagtc agccccatac gatataagtt gttactagtg cttggattct caccaataaa
      901 aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac
      961 tggatctatc aacaggagtc caagcgagct cgatatcaaa ttacgccccg ccctgccact
     1021 catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg
     1081 catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc
     1141 ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg
     1201 tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga
     1261 aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc
     1321 ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa
     1381 cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac
     1441 ggaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact
     1501 tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt
     1561 tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg
     1621 atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg
     1681 aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt
     1741 tggaacctct tacgtgccga tcaacgtctc attttcgcca gatatcgacg tctaagaaac
     1801 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca
     1861 cctcgagtcc ctatcagtga tagagattga catccctatc agtgatagag atactgagca
     1921 catcagcagg acgcactgac cgaattcatt aaagaggaga aaggtaccat gactatagag
     1981 ttcagccaca caaccggtct tctcctgctg gccatgcccg cactgctcct tatggccgct
     2041 gtaatcaaac ccaagcaagg tgctgcttat gcgcgcgctt atcggcaacg ggttcaatgg
     2101 accagttttg cggcctttgg cctggccctg ctcgctgtcg tcagttttct atttgcaagg
     2161 caacagaatc tgatgctcgg tgccggtagc ctccctgccg gattgggatt attggcgctt
     2221 tcaattcaag tcaatggcct gactctggtg ctggcaagtc tggttagttt tgtgcttagc
     2281 gtgattgccc gctacagcgt gcagtatctc gatggtgatc cccagcaggc ccgctttttc
     2341 cgcttgctgg cggtcaccgg cggatttttc ctgttggtcg tcatatcagg caatttggga
     2401 ttattcaccc tcgccatcat tgcgaccggt tttggtttgc atcgactgct ttctttttat
     2461 gccgatcggc cgcgcgcgat catggccaca cataaaaaat caattttcag ccggactgcc
     2521 gacgcccttt tgctcgcggc caccgtcttg atcggtcatc aaatcggtag tttggagttc
     2581 agccaaatca gcgcctatgt tcatgcgcaa gaccacctat ccattgcgtt gcacgtggct
     2641 gcttggctga tcgtgctggc cgccatcctt aaaagcgcgc aatttccgtt tcatggctgg
     2701 ctgattcagg tcatggaagc acccacgccg gtttccgcgc tcatgcacgc cggtgtcgtt
     2761 tacagcgggg caatcattgt gctgcgcacc agcgaattat tggccgccga cggtaccgcc
     2821 ctgttgcttc tggccctgat tggtttgatg accttggcta tcggctcgtt ggtcatgctg
     2881 acccaatcgg cgatcaaatc atcactggcc tggtctaccg ccgctcagct cggcttcatg
     2941 atgctcgaac tgggtttggg gttgtttggc ttggccttgc tgcacttggt tggccactcg
     3001 ctctacaagg cccatgcgtt cttgtcctcg ggcagcatga ccgatcacct gcgtcaggcc
     3061 aaagtgctca aaaaccgccc aatctctgtt gtggcctggt ttaccacggt gattgtgagc
     3121 ggcttgttca ccctcggtat cgcggcggcg atgggtttat cgatcgatca ggagccgatg
     3181 ttgcccgccg tgctgaccat catcgccttg gcgaccgctc agttgatgct caaggcattg
     3241 agccatggca catggcgtga aattctggtc gccgcaggcg cggcaatcgc catgaccggc
     3301 gtgtatgtct tcctgcatga ggtgttcatc acaggctttg ccgacacgct ggccgcgaca
     3361 ccgcaacgtg cgccgctgct cgatctgctg ttgatggcga tcacgatcat aaccttcctg
     3421 tttgttgcct ggcttcaagg ccccggcaaa accttgatga gcccggagcg acaattcgcc
     3481 ttgtttgttc acctcaataa cgggctgtat ctcgaccgct gggtcgaacg cttggccttc
     3541 cgtttctggc cggagaaagt tggccgcgca ccgaaaaaat cctgcgccgt gattccgcct
     3601 aacccatccg gtatcgagcc gtaaatccct gccacaaagg cacacaccat ttacccgagg
     3661 atttcgacca tgacgaccct gacatcatta caacgcagcg aagcgcaacg aaaccacatt
     3721 gttgacctga ttgacaaagc ctgcctgcgc atcgcgccca tctggccact ggattcgttt
     3781 gtcgccgtaa acccctatct tggtttgatt gatcaaccct ttgatacggt gggtcggtat
     3841 ctggaacaaa ccgtgggcga atccctattt atggatcacg gctggtttgc cgacaagatc
     3901 gcccaagggg aaatcaccga tgacgatttg gcgcaggcgg cgcagcaact ggacccatca
     3961 attagccttg ataccatcaa gcagcaactg gccgtccatc gacaacctgc acccgcgctg
     4021 cctttggtca caaacgagct cgatcgccgc gacgcgccgc cagtatctga atttgtcatc
     4081 gagcaagtca gccagttcat ggccaactac tatgatcgcg ggcaagccct ctggcactta
     4141 cccaaagaag ccagtgccag tttattcgcc caatggcgcc gctacacctt gattgaccgc
     4201 agcgccagcg ccgtcggctt aaagcaggtt cgccaacact tgcttgccgt cccaagcgat
     4261 gccattgatg cattgttctg ggcgctggac cagatcaatc tgcccgaaag tcgcctgccc
     4321 gactacctgt tcaccttatt aaaaaccatc ggtggctggg cgagctggtg ccgctatcta
     4381 cactttcaag ccggtttgca cggcgagtcg caacacgatt tacgcgattt gctgatcatt
     4441 cgcttggtct gggttgcttt ggtgatcaaa gaaaccagct cggctggacg gcaacaatgg
     4501 cgcgcgaagc taaacgattg gtttgatccg gccaaattgg tcgcaagccc ttcggcgaca
     4561 gctactgctt cgactaaggc tcaatccagc cggatcgatg aaatcctgct ggcggccgcc
     4621 gagcaggcgt ttcgacgccg gatcaatgcc ggcttgaacc gccagcctgc cgatgcaccc
     4681 gatcagcaag ccgaacgccc gactgttcag gccgcgtttt gcattgacgt gcgttccgag
     4741 gtgttccgcc gccacctcga agccagcagc ccggggctcg aaaccatcgg ttttgccggt
     4801 tttttcggcc tgccgattga ttattgccgc atgggcgaat cagaggctcg gctacaaaac
     4861 ccggtgctca tcaaccccgc ctaccgtgcg caggagacgg gcgatccggc cattgcccaa
     4921 catcgccatg cccgacaaag ccgaggggcc atctggaagc aattcaaact ttccgccgcc
     4981 tcgtgcttca cctttgtcga atccgcaggc ttgagctacg tgccgcgttt gctggccgat
     5041 tcactgggtt ggcatcgctc atcgctgccg cctgatgccc ccgggcttac ccctgaggag
     5101 cgtgcgcgtt tgcatcccca attggtcaaa ctcgatggcg gcgcactatc gacgcaggaa
     5161 aaagtcgatc tggccgaaaa agtactccgc gggctggggt taacgcacac ctttgcgccc
     5221 atcgtcctgc tcgccggaca cggcagctcc accaccaaca acccgcatcg cgcggggttg
     5281 gattgcggtg cctgcgccgg tcaggcaggc gatgtgaatg ctcgcgtcgc ggtgcagttg
     5341 ctcaatgagg ccgccgtgcg actggggctg atcgaacggg gtattgccat cccgcgtgac
     5401 acccggttcg tggccgccct gcacgacacc accacagacc acatcgaact actggatttg
     5461 gatcaatcag gcatcgagag tgatcaattg tcatccttga cccaggcgct caaacaagcc
     5521 ggtgagctca ctcgtctgga gcgtttggtg acgcttgagg cccaagtcga taccgttgat
     5581 gccgaaaaac aggccacgtt ccgagggcga gactggtcgc aagtacggcc ggaatggggg
     5641 ctagccggca atgctgcctt catcgccgca ccccgctggc gcacgcgcgg gttggatctt
     5701 ggcggccgcg ccttcctgca tgattacgat tggcgacacg ataaggagtt tggcgttttg
     5761 aacgtcatca tgaccgcgcc cttgatcgtc gcaaactgga tcaacctgca atactacggc
     5821 tccacggtcg ataatctgca tcaaggggcg ggcaataaag tgctgcataa tgtggtcggc
     5881 ggcaccgtcg gggtgattga aggcaatggc ggcgacctgc gagttggtct tgccatgcaa
     5941 tcgctgcacg atggtgaaca gtggcggcac gaaccgctgc ggctctcggc gtacattgag
     6001 gcgcccattg cagaaatcga taaaattatt gccggtcacg acatgctgaa cgcgttaatc
     6061 aacaaccgct ggatgcacat cctgcatatc gatgacaacg gcatcccgca tcgccgtcat
     6121 gcccacggtg actggcgacc ggaaccgatc tgaaatgtaa ctgtattcag cgatgacgaa
     6181 attcttagct attgtaatac tctagaggca tcaaataaaa cgaaaggctc agtcgaaaga
     6241 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc
     6301 gccgccgcta gcagttcgta gacggaaaca aacgcagaat ccaagcaaaa gggaataagg
     6361 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     6421 cagggttatg tccactgggt tcgtgccttc atccggatat ccaggaactg ttaagaccca
     6481 ctttcacatt taagttgttt ttctaatccg catatgatca attcaaggcc gaataagaag
     6541 gctggctctg caccttggtg atcaaataat tcgatagctt gtcgtaataa tggcggcata
     6601 ctatcagtag taggtgtttc cctttcttct ttagcgactt gatgctcttg atcttccaat
     6661 acgcaaccta aagtaaaatg ccccacagcg ctgagtgcat ataatgcatt ctctagtgaa
     6721 aaaccttgtt ggcataaaaa ggctaattga ttttcgagag tttcatactg tttttctgta
     6781 ggccgtgtac ctaaatgtac ttttgctcca tcgcgatgac ttagtaaagc acatctaaaa
     6841 cttttagcgt tattacgtaa aaaatcttgc cagctttccc cttctaaagg gcaaaagtga
     6901 gtatggtgcc tatctaacat ctcaatggct aaggcgtcga gcaaagcccg cttatttttt
     6961 acatgccaat acaatgtagg ctgctctaca cctagcttct gggcgagttt acgggttgtt
     7021 aaaccttcga ttccgacctc attaagcagc tctaatgcgc tgttaatcac tttactttta
     7081 tctaatctag acatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     7141 ctcatgagcg ga
//
