LOCUS       sequence-26311-e         682 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Boehm JS, Zhao JJ, Yao J, Kim SY, Firestein R, Dunn IF, Sjostrom SK,
            Garraway LA, Weremowicz S, Richardson AL, Greulich H, Stewart CJ, 
            Mulvey LA, Shen RR, Ambrogio L, Hirozane-Kishikawa T, Hill DE, Vidal
            M, Meyerson M, Grenier JK, Hinkle G, Root DE, Roberts TM, Lander ES,
            Polyak K, Hahn WC
  TITLE     Integrative Genomic Approaches Identify IKBKE as a Breast Cancer 
            Oncogene (Kinase Library Clones)
  JOURNAL   Cell. 2007 Jun 15. 129(6):1065-1079.
  PUBMED    17574021
REFERENCE   2  (bases 1 to 682)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Jun 15. 129(6):1065-1079."
COMMENT     Sequence Label: pWZL Neo Myr Flag FGR
FEATURES             Location/Qualifiers
     source          1..682
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      34..43
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             40..81
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             106..129
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    136..160
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tccttctcta ggcgccggcc ggatccagaa ttcgccacca tggggtcttc aaaatctaaa
       61 ccaaaggacc ccagccagcg ccggcgcagg atccgaggtt accttgacta caaagacgat
      121 gacgacaagc aattgacaag tttgtacaaa aaagttggca tgggctgtgt gttctgcaag
      181 aaattggagc cggtggccac ggccaaggag gatgctggcc tggaagggga cttcagaagc
      241 tacggggcag cagaccacta tgggcctgac cccactaagg cccggcctgc atcctcattt
      301 gcccacatcc ccaactacag caacttctcc tctcaggcca tcaaccctgg cttccttgat
      361 agtggcacca tcaggggtgt gtcagggatt ggggtgaccc tgttcattgc cctgtatgac
      421 tatgaggctc gaactgagga tgacctcacc ttcaccaagg gcgagaagtt ccacatcctg
      481 aacaatactg aaggtgactg gtgggaggct cggtctctca gctccggaaa aactggctgc
      541 attcccagca actacgtggc ccctgttgac tcaatccaag ctgaagagtg gtactttgga
      601 aagattggga gaaaggatgc agagaggcag ctgctttcac cangcaaccc ccanggggcc
      661 tttctcattc gggaaagcga ga
//
