LOCUS       sequence-263148-        4680 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4680)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLCO3A1
FEATURES             Location/Qualifiers
     source          1..4680
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2805..2904)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2921..2940)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2922..2940)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2945..2961)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2945..2961)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3063..3082)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3074..3883
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3141..3160)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4030..4618
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4519..4538
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatgcagg gcaagaagcc aggaggaagc tccggaggag gccggtccgg
      721 cgagctgcag ggcgatgagg cccagagaaa caagaagaag aagaagaagg tgagctgctt
      781 ctctaacatc aagatctttc tggtgtctga gtgtgcactg atgctggcac agggaaccgt
      841 gggagcatat ctggtgtctg tgctgaccac actggagcgg agattcaatc tgcagagcgc
      901 cgacgtgggc gtgatcgcat ctagctttga gatcggcaac ctggccctga tcctgttcgt
      961 gtcttacttt ggagcaaggg gacacaggcc aagactgatc ggatgcggag gaatcgtgat
     1021 ggcactgggc gccctgctga gcgccctgcc cgagttcctg acccaccagt acaagtatga
     1081 ggcaggagag atcagatggg gagcagaggg cagagacgtg tgcgccgcca atggaagcgg
     1141 aggcgacgag ggaccagacc cagatctgat ctgtaggaat cgcacagcca ccaacatgat
     1201 gtatctgctg ctgatcggag cacaggtgct gctgggaatc ggagccaccc ctgtgcagcc
     1261 actgggcgtg tcctatatcg acgatcacgt gaggcgcaag gattcctctc tgtacatcgg
     1321 catcctgttc accatgctgg tgtttggacc tgcatgcggc ttcatcctgg gatccttttg
     1381 tacaaagatc tacgtggacg ccgtgttcat cgatacatct aacctggaca tcaccccaga
     1441 cgatcctagg tggatcggag catggtgggg aggctttctg ctgtgcggcg ccctgctgtt
     1501 ctttagctcc ctgctgatgt tcggctttcc acagagcctg ccacctcact ccgagccagc
     1561 aatggagagc gagcaggcca tgctgtccga gcgggagtat gagagaccta agccaagcaa
     1621 tggcgtgctg aggcacccac tggagccaga ctctagcgcc tcctgtttcc agcagctgcg
     1681 cgtgatccct aaggtgacca agcacctgct gagcaaccca gtgtttacat gcatcatcct
     1741 ggccgcctgt atggagatcg cagtggtggc aggattcgca gcctttctgg gcaagtacct
     1801 ggagcagcag ttcaatctga ccacatcctc tgccaaccag ctgctgggca tgaccgccat
     1861 cccttgcgcc tgtctgggca tctttctggg cggcctgctg gtgaagaagc tgagcctgtc
     1921 cgccctggga gcaatccgga tggccatgct ggtgaatctg gtgtctacag cctgctatgt
     1981 gagcttcctg tttctgggat gtgatacagg accagtggca ggagtgaccg tgccttatgg
     2041 caactctaca gcaccaggaa gcgccctgga cccttacagc ccatgcaaca ataactgcga
     2101 gtgtcagaca gattccttca cccccgtgtg cggagcagac ggaatcacct acctgtctgc
     2161 ctgctttgcc ggctgtaata gcacaaacct gaccggatgc gcatgtctga ccacagtgcc
     2221 agcagagaat gcaaccgtgg tgcctggcaa gtgcccatcc cctggatgtc aggaggcctt
     2281 cctgacattt ctgtgcgtga tgtgcatctg ttccctgatc ggagcaatgg cacagacccc
     2341 atctgtgatc atcctgatcc ggacagtgtc ccctgagctg aagtcttacg ccctgggcgt
     2401 gctgttcctg ctgctgagac tgctgggctt catcccaccc cctctgatct ttggcgccgg
     2461 catcgattcc acatgcctgt tctggtctac cttttgcgga gagcagggag catgcgtgct
     2521 gtatgacaac gtggtgtaca gatatctgta cgtgagcatc gccatcgccc tgaagtcctt
     2581 cgcctttatc ctgtacacca caacctggca gtgtctgagg aagaattata agcggtacat
     2641 caagaaccac gagggcggcc tgtctaccag cgagttcttt gccagcacac tgaccctgga
     2701 taatctggga agggacccag tgccagcaaa ccagacacac cgcaccaagt tcatctacaa
     2761 tctggaggat cacgagtggt gcgagaacat ggagtccgtg ctggacccag ctttcttgta
     2821 caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat
     2881 cagtcaaaat aaaatcatta tttgccatcc agctgatatc ccctatagtg agtcgtatta
     2941 catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat
     3001 tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa
     3061 tacaaggggt gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa
     3121 catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc
     3181 gacaatctat cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa
     3241 aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt
     3301 tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac
     3361 cactgcgatc cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga
     3421 aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa
     3481 ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa
     3541 cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt
     3601 ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga
     3661 tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg
     3721 acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga
     3781 gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat
     3841 gaataaattg cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg
     3901 gttgtaacac tggcagagca ttacgctgac ttgacgggac ggcgcaagct catgaccaaa
     3961 atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     4021 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     4081 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     4141 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     4201 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     4261 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     4321 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     4381 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     4441 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     4501 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     4561 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     4621 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
//
