LOCUS       sequence-26319-e         673 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Boehm JS, Zhao JJ, Yao J, Kim SY, Firestein R, Dunn IF, Sjostrom SK,
            Garraway LA, Weremowicz S, Richardson AL, Greulich H, Stewart CJ, 
            Mulvey LA, Shen RR, Ambrogio L, Hirozane-Kishikawa T, Hill DE, Vidal
            M, Meyerson M, Grenier JK, Hinkle G, Root DE, Roberts TM, Lander ES,
            Polyak K, Hahn WC
  TITLE     Integrative Genomic Approaches Identify IKBKE as a Breast Cancer 
            Oncogene (Kinase Library Clones)
  JOURNAL   Cell. 2007 Jun 15. 129(6):1065-1079.
  PUBMED    17574021
REFERENCE   2  (bases 1 to 673)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Jun 15. 129(6):1065-1079."
COMMENT     Sequence Label: pWZL Neo Myr Flag GRK6
FEATURES             Location/Qualifiers
     source          1..673
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             39..80
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             105..128
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    135..159
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ccttctctag gcgccggccg gatccagaat tcgccaccat ggggtcttca aaatctaaac
       61 caaaggaccc cagccagcgc cggcgcagga tccgaggtta ccttgactac aaagacgatg
      121 acgacaagca attgacaagt ttgtacaaaa aagttggcat ggagctcgag aacatcgtag
      181 cgaacacggt gctactcaag gcccgggaag gtggcggtgg aaatcgcaaa ggcaaaagca
      241 agaaatggcg gcagatgctc cagttccctc acatcagcca gtgcgaagag ctgcggctca
      301 gcctcgagcg tgactatcac agcctgtgcg agcggcagcc cattgggcgc ctgctgttcc
      361 gagagttctg tgccacgagg ccggagctga gccgctgcgt cgccttcctg gatggggtgg
      421 ccgagtatga agtgaccccg gatgacaagc ggaaggcatg tgggcggcag ctaacgcaga
      481 attttctgag ccacacgggt cctgacctca tccctgaggt cccccggcag ctggtgacga
      541 actgcaccca gcggctggag cagggtccct gcaaagacct tttccaggaa ctcacccggc
      601 tgacccacga gtacctgagc gtggcccctt ttgccgacta cctcgacagc atctacttca
      661 accgtttcct gca
//
