LOCUS       sequence-263230-        2911 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Part of GoldenGate Yarrowia lipolytica toolkit for assembling an 
            expression plasmid for Y. lipolytic yeast. Coding sequence of 
            fluorescent protein in position 1 (G1_mTurquoise).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Larroude M, Park YK, Soudier P, Kubiak M, Nicaud JM, Rossignol T
  TITLE     A modular Golden Gate toolkit for Yarrowia lipolytica synthetic 
            biology.
  JOURNAL   Microb Biotechnol. 2019 May 31. doi: 10.1111/1751-7915.13427.
  PUBMED    31148366
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb 
            Biotechnol. 2019 May 31. doi: 10.1111/1751-7915.13427."
COMMENT     Sequence Label: GGE190
FEATURES             Location/Qualifiers
     source          1..2911
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     75..91
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     75..91
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      92..138
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     252..274
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     266..283
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     267..283
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1138..1154)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1138..1154)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1151..1173)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(1326..1914)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1406..1425)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2050..2865)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(2050..2865)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2773..2792
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2857..2876
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga cacaacgtgg
       61 ctttcccccg ccgctctaga actagtggat ccaaataaaa cgaaaggctc agtcgaaaga
      121 ctgggccttt cgttttatct gttgtttgtc gcattatacg agacgtccag gttgggatac
      181 ctgaaacaaa acccatcgta cggccaagga agtctccaat aactgtgatc caccacaagc
      241 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtcatgcat aatccgcacg
      301 catctggaat aaggaagtgc cattccgcct gacctggggt ctctaatgat ggtttcaaag
      361 ggagaggaac tgttcacagg cgtcgtcccg atacttgtgg aacttgacgg tgacgtaaac
      421 ggacataagt tttctgtctc tggtgagggt gaaggtgacg ccacctacgg aaagctgact
      481 ctgaaattca tttgcaccac cggaaagctt ccagttcctt ggcctaccct ggtcactacc
      541 ctgtcttggg gtgtgcaatg tttcgctcga taccccgatc acatgaagca gcacgacttt
      601 ttcaagtctg ccatgcccga gggatacgtt caggagcgaa ctatcttctt caaggacgat
      661 ggtaactaca agactcgagc tgaggttaag ttcgagggtg acactctggt caaccgaatt
      721 gaacttaagg gtattgactt caaggaggat ggcaacattc tgggtcacaa gctggagtat
      781 aactacttta gtgacaatgt ctacatcacc gcggataagc agaagaacgg aatcaaagcc
      841 aactttaaga ttagacataa tattgaggat gggggagtgc agctggccga tcactaccag
      901 cagaacactc ccatcggtga cggccccgtc ctgctgcctg acaaccacta cctctcgacc
      961 cagagcaagt tgtccaagga ccctaacgag aagcgtgacc acatggtgct cctggagttt
     1021 gtgacagctg ctggtatcac cctcggcatg gacgagctct acaagtaatc taagagaccc
     1081 caggctaggt ggaggctcag tgatgataag tctgcgatgg tggatgcatg tgtcatggtc
     1141 atagctgttt cctgtgtgaa attgttatcc gctcagaggg cacaatccta ttccgcgcta
     1201 tccgacaatc tccaagacat taggtggagt tcagttcggc gtatggcata tgtcgctgga
     1261 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     1321 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     1381 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     1441 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     1501 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     1561 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     1621 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     1681 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     1741 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     1801 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     1861 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     1921 cctttgatct tttctacggg gtctgacgct ctattcaaca aagccgccgt cccgtcaagt
     1981 cagcgtaaat gggtaggggg cttcaaatcg tccgctctgc cagtgttaca accaattaac
     2041 aaattctgat tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg
     2101 attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag
     2161 gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc
     2221 aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg
     2281 agtgacgact gaatccggtg agaatggcaa aagcttatgc atttctttcc agacttgttc
     2341 aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat
     2401 tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac
     2461 aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga
     2521 atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa
     2581 ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt
     2641 cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg
     2701 tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga
     2761 ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt
     2821 taatcgcggc ctcgagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt
     2881 actgtttatg taagcagaca gttttattgt t
//
