LOCUS       sequence-263246-       13705 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for c-myc-tagged PDGFRA (V242K mutant).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13705)
  AUTHORS   Park J, Gill KS, Aghajani AA, Heredia JD, Choi H, Oberstein A, 
            Procko E
  TITLE     Engineered receptors for human cytomegalovirus that are orthogonal 
            to normal human biology.
  JOURNAL   PLoS Pathog. 2020 Jun 19;16(6):e1008647. doi: 
            10.1371/journal.ppat.1008647. eCollection 2020 Jun.
  PUBMED    32559251
REFERENCE   2  (bases 1 to 13705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog."; date: "2020-06-19"; volume: "16(6):e1008647. doi"; pages: 
            " 10.1371/journal.ppat.1008647. eCollection 2020 Jun"
COMMENT     Sequence Label: pCEP4-myc-PDGFRalpha-V242K
FEATURES             Location/Qualifiers
     source          1..13705
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             61..90
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    3338..3472
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3362..3381)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3416..3435
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3614..3636)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      4222..6011
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(6317..8242)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(8677..8695)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8763..8867
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8868..8936,8937..9728)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9086..9105)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9899..10487
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10388..10407
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        11104..11249
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             11289..12314
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             11289..12314
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     complement(12335..12354)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    12379..12427
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        13080..13459
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        13460..13663
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     13614..13634
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     13660..13684
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gctagcgcca ccatgaagac catcatcgcc ctgagctaca tcttctgcct ggtgttcgcc
       61 gagcagaaat tgatcagcga ggaggacctg ggcagccctg gaggcgcctc tggccagctt
      121 tcattaccct ctatccttcc aaatgaaaat gaaaaggttg tgcagctgaa ttcatccttt
      181 tctctgagat gctttgggga gagtgaagtg agctggcagt accccatgtc tgaagaagag
      241 agctccgatg tggaaatcag aaatgaagaa aacaacagcg gcctttttgt gacggtcttg
      301 gaagtgagca gtgcctcggc ggcccacaca gggttgtaca cttgctatta caaccacact
      361 cagacagaag agaatgagct tgaaggcagg cacatttaca tctatgtgcc agacccagat
      421 gtagcctttg tacctctagg aatgacggat tatttagtca tcgtggagga tgatgattct
      481 gccattatac cttgtcgcac aactgatccc gagactcctg taaccttaca caacagtgag
      541 ggggtggtac ctgcctccta tgacagcaga cagggcttta atgggacctt cactgtaggg
      601 ccctatatct gtgaggccac cgtcaaagga aagaagttcc agaccatccc atttaatgtt
      661 tatgctttaa aagcaacatc agagctggat ctagaaatgg aagctcttaa aaccgtgtat
      721 aagtcagggg aaacgattgt ggtcacctgt gctgttttta acaatgagaa ggttgacctt
      781 caatggactt accctggaga agtgaaaggc aaaggcatca caatgctgga agaaatcaaa
      841 gtcccatcca tcaaattggt gtacactttg acggtccccg aggccacggt gaaagacagt
      901 ggagattacg aatgtgctgc ccgccaggct accagggagg tcaaagaaat gaagaaagtc
      961 actatttctg tccatgagaa aggtttcatt gaaatcaaac ccaccttcag ccagttggaa
     1021 gctgtcaacc tgcatgaagt caaacatttt gttgtagagg tgcgggccta cccacctccc
     1081 aggatatcct ggctgaaaaa caatctgact ctgattgaaa atctcactga gatcaccact
     1141 gatgtggaaa agattcagga aataaggtat cgaagcaaat taaagctgat ccgtgctaag
     1201 gaagaagaca gtggccatta tactattgta gctcaaaatg aagatgctgt gaagagctat
     1261 acttttgaac tgttaactca agttccttca tccattctgg acttggtcga tgatcaccat
     1321 ggctcaactg ggggacagac cgtacggtgc acagctgaag gcacgccgct tcctgatatt
     1381 gagtggatga tatgcaaaga tattaagaaa tgtaataatg aaacttcctg gactattttg
     1441 gccaacaatg tctcaaacat catcacggag atccactccc gagacaggag taccgtggag
     1501 ggccgtgtga ctttcgccaa agtggaggag accatcgccg tgcgatgcct ggctaagaat
     1561 ctccttggag ctgagaaccg agagctgaag ctggtggctc ccaccctgcg ttctgaactc
     1621 acggtggctg ctgcagtcct ggtgctgttg gtgattgtga tcatctcact tattgtcctg
     1681 gttgtcattt ggaaacagaa accgaggtat gaaattcgct ggagggtcat tgaatcaatc
     1741 agcccggatg gacatgaata tatttatgtg gacccgatgc agctgcctta tgactcaaga
     1801 tgggagtttc caagagatgg actagtgctt ggtcgggtct tggggtctgg agcgtttggg
     1861 aaggtggttg aaggaacagc ctatggatta agccggtccc aacctgtcat gaaagttgca
     1921 gtgaagatgc taaaacccac ggccagatcc agtgaaaaac aagctctcat gtctgaactg
     1981 aagataatga ctcacctggg gccacatttg aacattgtaa acttgctggg agcctgcacc
     2041 aagtcaggcc ccatttacat catcacagag tattgcttct atggagattt ggtcaactat
     2101 ttgcataaga atagggatag cttcctgagc caccacccag agaagccaaa gaaagagctg
     2161 gatatctttg gattgaaccc tgctgatgaa agcacacgga gctatgttat tttatctttt
     2221 gaaaacaatg gtgactacat ggacatgaag caggctgata ctacacagta tgtccccatg
     2281 ctagaaagga aagaggtttc taaatattcc gacatccaga gatcactcta tgatcgtcca
     2341 gcctcatata agaagaaatc tatgttagac tcagaagtca aaaacctcct ttcagatgat
     2401 aactcagaag gccttacttt attggatttg ttgagcttca cctatcaagt tgcccgagga
     2461 atggagtttt tggcttcaaa aaattgtgtc caccgtgatc tggctgctcg caacgtcctc
     2521 ctggcacaag gaaaaattgt gaagatctgt gactttggcc tggccagaga catcatgcat
     2581 gattcgaact atgtgtcgaa aggcagtacc tttctgcccg tgaagtggat ggctcctgag
     2641 agcatctttg acaacctcta caccacactg agtgatgtct ggtcttatgg cattctgctc
     2701 tgggagatct tttcccttgg tggcacccct taccccggca tgatggtgga ttctactttc
     2761 tacaataaga tcaagagtgg gtaccggatg gccaagcctg accacgctac cagtgaagtc
     2821 tacgagatca tggtgaaatg ctggaacagt gagccggaga agagaccctc cttttaccac
     2881 ctgagtgaga ttgtggagaa tctgctgcct ggacaatata aaaagagtta tgaaaaaatt
     2941 cacctggact tcctgaagag tgaccatcct gctgtggcac gcatgcgtgt ggactcagac
     3001 aatgcataca ttggtgtcac ctacaaaaac gaggaagaca agctgaagga ctgggagggt
     3061 ggtctggatg agcagagact gagcgctgac agtggctaca tcattcctct gcctgacatt
     3121 gaccctgtcc ctgaggagga ggacctgggc aagaggaaca gacacagctc gcagacctct
     3181 gaagagagtg ccattgagac gggttccagc agttccacct tcatcaagag agaggacgag
     3241 accattgaag acatcgacat gatggacgac atcggcatag actcttcaga cctggtggaa
     3301 gacagcttcc tgtaatgact cgaggccggc aaggccggat ccagacatga taagatacat
     3361 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
     3421 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
     3481 caattgcatt cattttatgt ttcaggttca gggggaggtg gggaggtttt ttaaagcaag
     3541 taaaacctct acaaatgtgg tatggctgat tatgatccgg ctgcctcgcg cgtttcggtg
     3601 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     3661 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     3721 gcgcagccat gaccggtcga ctctagagcc ccgccgccgg acgaactaaa cctgactacg
     3781 gcatctctgc cccttcttcg ctggtacgag gagcgctttt gttttgtatt cggggcagtg
     3841 catgtaatcc cttcagttgg ttggtacaac ttgccaactg ggccctgttc cacatgtgac
     3901 acgggggggg accaaacaca aaggggttct ctgactgtag ttgacatcct tataaatgga
     3961 tgtgcacatt tgccaacact gagtggcttt catcctggag cagactttgc atgctgtgga
     4021 ctgcaacaca acattgcctt tatgtgtaac tcttggctga agctcttaca ccaatgctgg
     4081 gggacatgta cctcccaggg gcccaggaag actacgggag gctacaccaa cgtcaatcag
     4141 aggggcctgt gtagctaccg ataagcggac cctcaagagg gcattagcaa tagtgtttat
     4201 aaggccccct tgttaaccct aaacgggtag catatgcttc ccgggtagta gtatatacta
     4261 tccagactaa ccctaattca atagcatatg ttacccaacg ggaagcatat gctatcgaat
     4321 tagggttagt aaaagggtcc taaggaacag cgatatctcc caccccatga gctgtcacgg
     4381 ttttatttac atggggtcag gattccacga gggtagtgaa ccattttagt cacaagggca
     4441 gtggctgaag atcaaggagc gggcagtgaa ctctcctgaa tcttcgcctg cttcttcatt
     4501 ctccttcgtt tagctaatag aataactgct gagttgtgaa cagtaaggtg tatgtgaggt
     4561 gctcgaaaac aaggtttcag gtgacgcccc cagaataaaa tttggacggg gggttcagtg
     4621 gtggcattgt gctatgacac caatataacc ctcacaaacc ccttgggcaa taaatactag
     4681 tgtaggaatg aaacattctg aatatcttta acaatagaaa tccatggggt ggggacaagc
     4741 cgtaaagact ggatgtccat ctcacacgaa tttatggcta tgggcaacac ataatcctag
     4801 tgcaatatga tactggggtt attaagatgt gtcccaggca gggaccaaga caggtgaacc
     4861 atgttgttac actctatttg taacaagggg aaagagagtg gacgccgaca gcagcggact
     4921 ccactggttg tctctaacac ccccgaaaat taaacggggc tccacgccaa tggggcccat
     4981 aaacaaagac aagtggccac tctttttttt gaaattgtgg agtgggggca cgcgtcagcc
     5041 cccacacgcc gccctgcggt tttggactgt aaaataaggg tgtaataact tggctgattg
     5101 taaccccgct aaccactgcg gtcaaaccac ttgcccacaa aaccactaat ggcaccccgg
     5161 ggaatacctg cataagtagg tgggcgggcc aagatagggg cgcgattgct gcgatctgga
     5221 ggacaaatta cacacacttg cgcctgagcg ccaagcacag ggttgttggt cctcatattc
     5281 acgaggtcgc tgagagcacg gtgggctaat gttgccatgg gtagcatata ctacccaaat
     5341 atctggatag catatgctat cctaatctat atctgggtag cataggctat cctaatctat
     5401 atctgggtag catatgctat cctaatctat atctgggtag tatatgctat cctaatttat
     5461 atctgggtag cataggctat cctaatctat atctgggtag catatgctat cctaatctat
     5521 atctgggtag tatatgctat cctaatctgt atccgggtag catatgctat cctaatagag
     5581 attagggtag tatatgctat cctaatttat atctgggtag catatactac ccaaatatct
     5641 ggatagcata tgctatccta atctatatct gggtagcata tgctatccta atctatatct
     5701 gggtagcata ggctatccta atctatatct gggtagcata tgctatccta atctatatct
     5761 gggtagtata tgctatccta atttatatct gggtagcata ggctatccta atctatatct
     5821 gggtagcata tgctatccta atctatatct gggtagtata tgctatccta atctgtatcc
     5881 gggtagcata tgctatcctc atgcatatac agtcagcata tgatacccag tagtagagtg
     5941 ggagtgctat cctttgcata tgccgccacc tcccaagggg gcgtgaattt tcgctgcttg
     6001 tccttttcct gcatgctggt tgctcccatt cttaggtgaa tttaaggagg ccaggctaaa
     6061 gccgtcgcat gtctgattgc tcaccaggta aatgtcgcta atgttttcca acgcgagaag
     6121 gtgttgagcg cggagctgag tgacgtgaca acatgggtat gcccaattgc cccatgttgg
     6181 gaggacgaaa atggtgacaa gacagatggc cagaaataca ccaacagcac gcatgatgtc
     6241 tactggggat ttattcttta gtgcggggga atacacggct tttaatacga ttgagggcgt
     6301 ctcctaacaa gttacatcac tcctgccctt cctcaccctc atctccatca cctccttcat
     6361 ctccgtcatc tccgtcatca ccctccgcgg cagccccttc caccataggt ggaaaccagg
     6421 gaggcaaatc tactccatcg tcaaagctgc acacagtcac cctgatattg caggtaggag
     6481 cgggctttgt cataacaagg tccttaatcg catccttcaa aacctcagca aatatatgag
     6541 tttgtaaaaa gaccatgaaa taacagacaa tggactccct tagcgggcca ggttgtgggc
     6601 cgggtccagg ggccattcca aaggggagac gactcaatgg tgtaagacga cattgtggaa
     6661 tagcaagggc agttcctcgc cttaggttgt aaagggaggt cttactacct ccatatacga
     6721 acacaccggc gacccaagtt ccttcgtcgg tagtcctttc tacgtgactc ctagccagga
     6781 gagctcttaa accttctgca atgttctcaa atttcgggtt ggaacctcct tgaccacgat
     6841 gctttccaaa ccaccctcct tttttgcgcc tgcctccatc accctgaccc cggggtccag
     6901 tgcttgggcc ttctcctggg tcatctgcgg ggccctgctc tatcgctccc gggggcacgt
     6961 caggctcacc atctgggcca ccttcttggt ggtattcaaa ataatcggct tcccctacag
     7021 ggtggaaaaa tggccttcta cctggagggg gcctgcgcgg tggagacccg gatgatgatg
     7081 actgactact gggactcctg ggcctctttt ctccacgtcc acgacctctc cccctggctc
     7141 tttcacgact tccccccctg gctctttcac gtcctctacc ccggcggcct ccactacctc
     7201 ctcgaccccg gcctccacta cctcctcgac cccggcctcc actgcctcct cgaccccggc
     7261 ctccacctcc tgctcctgcc cctcctgctc ctgcccctcc tcctgctcct gcccctcctg
     7321 cccctcctgc tcctgcccct cctgcccctc ctgctcctgc ccctcctgcc cctcctgctc
     7381 ctgcccctcc tgcccctcct cctgctcctg cccctcctgc ccctcctcct gctcctgccc
     7441 ctcctgcccc tcctgctcct gcccctcctg cccctcctgc tcctgcccct cctgcccctc
     7501 ctgctcctgc ccctcctgct cctgcccctc ctgctcctgc ccctcctgct cctgcccctc
     7561 ctgcccctcc tgcccctcct cctgctcctg cccctcctgc tcctgcccct cctgcccctc
     7621 ctgcccctcc tgctcctgcc cctcctcctg ctcctgcccc tcctgcccct cctgcccctc
     7681 ctcctgctcc tgcccctcct gcccctcctc ctgctcctgc ccctcctcct gctcctgccc
     7741 ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc tgcccctcct cctgctcctg
     7801 cccctcctcc tgctcctgcc cctcctgccc ctcctgcccc tcctcctgct cctgcccctc
     7861 ctcctgctcc tgcccctcct gcccctcctg cccctcctgc ccctcctcct gctcctgccc
     7921 ctcctcctgc tcctgcccct cctgctcctg cccctcccgc tcctgctcct gctcctgttc
     7981 caccgtgggt ccctttgcag ccaatgcaac ttggacgttt ttggggtctc cggacaccat
     8041 ctctatgtct tggccctgat cctgagccgc ccggggctcc tggtcttccg cctcctcgtc
     8101 ctcgtcctct tccccgtcct cgtccatggt tatcaccccc tcttctttga ggtccactgc
     8161 cgccggagcc ttctggtcca gatgtgtctc ccttctctcc taggccattt ccaggtcctg
     8221 tacctggccc ctcgtcagac atgattcaca ctaaaagaga tcaatagaca tctttattag
     8281 acgacgctca gtgaatacag ggagtgcaga ctcctgcccc ctccaacagc ccccccaccc
     8341 tcatcccctt catggtcgct gtcagacaga tccaggtctg aaaattcccc atcctccgaa
     8401 ccatcctcgt cctcatcacc aattactcgc agcccggaaa actcccgctg aacatcctca
     8461 agatttgcgt cctgagcctc aagccaggcc tcaaattcct cgtccccctt tttgctggac
     8521 ggtagggatg gggattctcg ggacccctcc tcttcctctt caaggtcacc agacagagat
     8581 gctactgggg caacggaaga aaagctgggt gcggcctgtg aggatcagct tatcgatgat
     8641 aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat
     8701 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     8761 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     8821 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     8881 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     8941 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     9001 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     9061 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg
     9121 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     9181 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     9241 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     9301 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     9361 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg
     9421 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     9481 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     9541 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     9601 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     9661 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     9721 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     9781 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     9841 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     9901 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     9961 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
    10021 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
    10081 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
    10141 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
    10201 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
    10261 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
    10321 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
    10381 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
    10441 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
    10501 cggccttttt acggttcctg gccttttgct gcgccgcgtg cggctgctgg agatggcgga
    10561 cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg
    10621 attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag
    10681 gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg
    10741 gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc
    10801 gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga
    10861 agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc
    10921 ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg
    10981 aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cctgcttcat ccccgtggcc
    11041 cgttgctcgc gtttgctggc ggtgtccccg gaagaaatat atttgcatgt ctttagttct
    11101 atgatgacac aaaccccgcc cagcgtcttg tcattggcga attcgaacac gcagatgcag
    11161 tcggggcggc gcggtcccag gtccacttcg catattaagg tgacgcgtgt ggcctcgaac
    11221 accgagcgac cctgcagcga cccgcttaac agcgtcaaca gcgtgccgca gatcccgggc
    11281 aatgagatat gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa
    11341 agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca
    11401 gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct
    11461 acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc
    11521 ttgacattgg ggaattcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg
    11581 tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg
    11641 ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac
    11701 cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc
    11761 atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc
    11821 tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg
    11881 atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga
    11941 gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt
    12001 ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag
    12061 gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct
    12121 tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc
    12181 gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga
    12241 ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccga
    12301 gggcaaagga ataggggaga tgggggaggc taactgaaac acggaaggag acaataccgg
    12361 aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggg tgttgggtcg
    12421 tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga
    12481 ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg
    12541 ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat agccactggc
    12601 cccgtgggtt agggacgggg tcccccatgg ggaatggttt atggttcgtg ggggttatta
    12661 ttttgggcgt tgcgtggggt ctggtccacg actggactga gcagacagac ccatggtttt
    12721 tggatggcct gggcatggac cgcatgtact ggcgcgacac gaacaccggg cgtctgtggc
    12781 tgccaaacac ccccgacccc caaaaaccac cgcgcggatt tctggcgtgc caagctagtc
    12841 gaccaattct catgtttgac agcttatcat cgcagatccg ggcaacgttg ttgcattgct
    12901 gcaggcgcag aactggtagg tatggaagat ctatacattg aatcaatatt ggcaattagc
    12961 catattagtc attggttata tagcataaat caatattggc tattggccat tgcatacgtt
    13021 gtatctatat cataatatgt acatttatat tggctcatgt ccaatatgac cgccatgttg
    13081 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
    13141 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
    13201 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
    13261 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
    13321 agtgtatcat atgccaagtc cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
    13381 gcattatgcc cagtacatga ccttacggga ctttcctact tggcagtaca tctacgtatt
    13441 agtcatcgct attaccatgg tgatgcggtt ttggcagtac accaatgggc gtggatagcg
    13501 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
    13561 gcaccaaaat caacgggact ttccaaaatg tcgtaataac cccgccccgt tgacgcaaat
    13621 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca
    13681 gatctctaga agctgggtac cagct
//
