LOCUS       sequence-263270-       13729 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for c-myc-tagged PDGFRB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13729)
  AUTHORS   Park J, Gill KS, Aghajani AA, Heredia JD, Choi H, Oberstein A, 
            Procko E
  TITLE     Engineered receptors for human cytomegalovirus that are orthogonal 
            to normal human biology.
  JOURNAL   PLoS Pathog. 2020 Jun 19;16(6):e1008647. doi: 
            10.1371/journal.ppat.1008647. eCollection 2020 Jun.
  PUBMED    32559251
REFERENCE   2  (bases 1 to 13729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog."; date: "2020-06-19"; volume: "16(6):e1008647. doi"; pages: 
            " 10.1371/journal.ppat.1008647. eCollection 2020 Jun"
COMMENT     Sequence Label: pCEP4-myc-PDGFRbeta
FEATURES             Location/Qualifiers
     source          1..13729
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             61..90
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1555..1701
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     polyA_signal    3362..3496
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3386..3405)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3440..3459
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3638..3660)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      4246..6035
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(6341..8266)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(8701..8719)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8787..8891
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8892..8960,8961..9752)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9110..9129)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9923..10511
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10412..10431
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        11128..11273
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             11313..12338
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             11313..12338
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     complement(12359..12378)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    12403..12451
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        13104..13483
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        13484..13687
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     13638..13658
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     13684..13708
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gctagcgcca ccatgaagac catcatcgcc ctgagctaca tcttctgcct ggtgttcgcc
       61 gagcagaaat tgatcagcga ggaggacctg ggcagccctg gaggcgcctc tggcctggtc
      121 gtcacacccc cggggccaga gcttgtcctc aatgtctcca gcaccttcgt tctgacctgc
      181 tcgggttcag ctccggtggt gtgggaacgg atgtcccagg agcccccaca ggaaatggcc
      241 aaggcccagg atggcacctt ctccagcgtg ctcacactga ccaacctcac tgggctagac
      301 acgggagaat acttttgcac ccacaatgac tcccgtggac tggagaccga tgagcggaaa
      361 cggctctaca tctttgtgcc agatcccacc gtgggcttcc tccctaatga tgccgaggaa
      421 ctattcatct ttctcacgga aataactgag atcaccattc catgccgagt aacagaccca
      481 cagctggtgg tgacactgca cgagaagaaa ggggacgttg cactgcctgt cccctatgat
      541 caccaacgtg gcttttctgg tatctttgag gacagaagct acatctgcaa aaccaccatt
      601 ggggacaggg aggtggattc tgatgcctac tatgtctaca gactccaggt gtcatccatc
      661 aacgtctctg tgaacgcagt gcagactgtg gtccgccagg gtgagaacat caccctcatg
      721 tgcattgtga tcgggaatga ggtggtcaac ttcgagtgga catacccccg caaagaaagt
      781 gggcggctgg tggagccggt gactgacttc ctcttggata tgccttacca catccgctcc
      841 atcctgcaca tccccagtgc cgagttagaa gactcgggga cctacacctg caatgtgacg
      901 gagagtgtga atgaccatca ggatgaaaag gccatcaaca tcaccgtggt tgagagcggc
      961 tacgtgcggc tcctgggaga ggtgggcaca ctacaatttg ctgagctgca tcggagccgg
     1021 acactgcagg tagtgttcga ggcctaccca ccgcccactg tcctgtggtt caaagacaac
     1081 cgcaccctgg gcgactccag cgctggcgaa atcgccctgt ccacgcgcaa cgtgtcggag
     1141 acccggtatg tgtcagagct gacactggtt cgcgtgaagg tggcagaggc tggccactac
     1201 accatgcggg ccttccatga ggatgctgag gtccagctct ccttccagct acagatcaat
     1261 gtccctgtcc gagtgctgga gctaagtgag agccaccctg acagtgggga acagacagtc
     1321 cgctgtcgtg gccggggcat gccccagccg aacatcatct ggtctgcctg cagagacctc
     1381 aaaaggtgtc cacgtgagct gccgcccacg ctgctgggga acagttccga agaggagagc
     1441 cagctggaga ctaacgtgac gtactgggag gaggagcagg agtttgaggt ggtgagcaca
     1501 ctgcgtctgc agcacgtgga tcggccactg tcggtgcgct gcacgctgcg caacgctgtg
     1561 ggccaggaca cgcaggaggt catcgtggtg ccacactcct tgccctttaa ggtggtggtg
     1621 atctcagcca tcctggccct ggtggtgctc accatcatct cccttatcat cctcatcatg
     1681 ctttggcaga agaagccacg ttacgagatc cgatggaagg tgattgagtc tgtgagctct
     1741 gacggccatg agtacatcta cgtggacccc atgcagctgc cctatgactc cacgtgggag
     1801 ctgccgcggg accagcttgt gctgggacgc accctcggct ctggggcctt tgggcaggtg
     1861 gtggaggcca cggctcatgg cctgagccat tctcaggcca cgatgaaagt ggccgtcaag
     1921 atgcttaaat ccacagcccg cagcagtgag aagcaagccc ttatgtcgga gctgaagatc
     1981 atgagtcacc ttgggcccca cctgaacgtg gtcaacctgt tgggggcctg caccaaagga
     2041 ggacccatct atatcatcac tgagtactgc cgctacggag acctggtgga ctacctgcac
     2101 cgcaacaaac acaccttcct gcagcaccac tccgacaagc gccgcccgcc cagcgcggag
     2161 ctctacagca atgctctgcc cgttgggctc cccctgccca gccatgtgtc cttgaccggg
     2221 gagagcgacg gtggctacat ggacatgagc aaggacgagt cggtggacta tgtgcccatg
     2281 ctggacatga aaggagacgt caaatatgca gacatcgagt cctccaacta catggcccct
     2341 tacgataact acgttccctc tgcccctgag aggacctgcc gagcaacttt gatcaacgag
     2401 tctccagtgc taagctacat ggacctcgtg ggcttcagct accaggtggc caatggcatg
     2461 gagtttctgg cctccaagaa ctgcgtccac agagacctgg cggctaggaa cgtgctcatc
     2521 tgtgaaggca agctggtcaa gatctgtgac tttggcctgg ctcgggacat catgcgggac
     2581 tcgaattaca tctccaaagg cagcaccttt ttgcctttaa agtggatggc tccggagagc
     2641 atcttcaaca gcctctacac caccctgagc gacgtgtggt ccttcgggat cctgctctgg
     2701 gagatcttca ccttgggtgg caccccttac ccagagctgc ccatgaacga gcagttctac
     2761 aatgccatca aacggggtta ccgcatggcc cagcctgccc atgcctccga cgagatctat
     2821 gagatcatgc agaagtgctg ggaagagaag tttgagattc ggcccccctt ctcccagctg
     2881 gtgctgcttc tggagagact gttgggcgaa ggttacaaaa agaagtacca gcaggtggat
     2941 gaggagtttc tgaggagtga ccacccagcc atccttcggt cccaggcccg cttgcctggg
     3001 ttccatggcc tccgatctcc cctggacacc agctccgtcc tctatactgc cgtgcagccc
     3061 aatgagggtg acaacgacta tatcatcccc ctgcctgacc ccaaacccga ggttgctgac
     3121 gagggcccac tggagggttc ccccagccta gccagctcca ccctgaatga agtcaacacc
     3181 tcctcaacca tctcctgtga cagccccctg gagccccagg acgaaccaga gccagagccc
     3241 cagcttgagc tccaggtgga gccggagcca gagctggaac agttgccgga ttcggggtgc
     3301 cctgcgcctc gggcggaagc agaggatagc ttcctgtaat gactcgaggc cggcaaggcc
     3361 ggatccagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
     3421 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     3481 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
     3541 ggtggggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat
     3601 ccggctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
     3661 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
     3721 cagcgggtgt tggcgggtgt cggggcgcag ccatgaccgg tcgactctag agccccgccg
     3781 ccggacgaac taaacctgac tacggcatct ctgccccttc ttcgctggta cgaggagcgc
     3841 ttttgttttg tattcggggc agtgcatgta atcccttcag ttggttggta caacttgcca
     3901 actgggccct gttccacatg tgacacgggg ggggaccaaa cacaaagggg ttctctgact
     3961 gtagttgaca tccttataaa tggatgtgca catttgccaa cactgagtgg ctttcatcct
     4021 ggagcagact ttgcatgctg tggactgcaa cacaacattg cctttatgtg taactcttgg
     4081 ctgaagctct tacaccaatg ctgggggaca tgtacctccc aggggcccag gaagactacg
     4141 ggaggctaca ccaacgtcaa tcagaggggc ctgtgtagct accgataagc ggaccctcaa
     4201 gagggcatta gcaatagtgt ttataaggcc cccttgttaa ccctaaacgg gtagcatatg
     4261 cttcccgggt agtagtatat actatccaga ctaaccctaa ttcaatagca tatgttaccc
     4321 aacgggaagc atatgctatc gaattagggt tagtaaaagg gtcctaagga acagcgatat
     4381 ctcccacccc atgagctgtc acggttttat ttacatgggg tcaggattcc acgagggtag
     4441 tgaaccattt tagtcacaag ggcagtggct gaagatcaag gagcgggcag tgaactctcc
     4501 tgaatcttcg cctgcttctt cattctcctt cgtttagcta atagaataac tgctgagttg
     4561 tgaacagtaa ggtgtatgtg aggtgctcga aaacaaggtt tcaggtgacg cccccagaat
     4621 aaaatttgga cggggggttc agtggtggca ttgtgctatg acaccaatat aaccctcaca
     4681 aaccccttgg gcaataaata ctagtgtagg aatgaaacat tctgaatatc tttaacaata
     4741 gaaatccatg gggtggggac aagccgtaaa gactggatgt ccatctcaca cgaatttatg
     4801 gctatgggca acacataatc ctagtgcaat atgatactgg ggttattaag atgtgtccca
     4861 ggcagggacc aagacaggtg aaccatgttg ttacactcta tttgtaacaa ggggaaagag
     4921 agtggacgcc gacagcagcg gactccactg gttgtctcta acacccccga aaattaaacg
     4981 gggctccacg ccaatggggc ccataaacaa agacaagtgg ccactctttt ttttgaaatt
     5041 gtggagtggg ggcacgcgtc agcccccaca cgccgccctg cggttttgga ctgtaaaata
     5101 agggtgtaat aacttggctg attgtaaccc cgctaaccac tgcggtcaaa ccacttgccc
     5161 acaaaaccac taatggcacc ccggggaata cctgcataag taggtgggcg ggccaagata
     5221 ggggcgcgat tgctgcgatc tggaggacaa attacacaca cttgcgcctg agcgccaagc
     5281 acagggttgt tggtcctcat attcacgagg tcgctgagag cacggtgggc taatgttgcc
     5341 atgggtagca tatactaccc aaatatctgg atagcatatg ctatcctaat ctatatctgg
     5401 gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg
     5461 gtagtatatg ctatcctaat ttatatctgg gtagcatagg ctatcctaat ctatatctgg
     5521 gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat ctgtatccgg
     5581 gtagcatatg ctatcctaat agagattagg gtagtatatg ctatcctaat ttatatctgg
     5641 gtagcatata ctacccaaat atctggatag catatgctat cctaatctat atctgggtag
     5701 catatgctat cctaatctat atctgggtag cataggctat cctaatctat atctgggtag
     5761 catatgctat cctaatctat atctgggtag tatatgctat cctaatttat atctgggtag
     5821 cataggctat cctaatctat atctgggtag catatgctat cctaatctat atctgggtag
     5881 tatatgctat cctaatctgt atccgggtag catatgctat cctcatgcat atacagtcag
     5941 catatgatac ccagtagtag agtgggagtg ctatcctttg catatgccgc cacctcccaa
     6001 gggggcgtga attttcgctg cttgtccttt tcctgcatgc tggttgctcc cattcttagg
     6061 tgaatttaag gaggccaggc taaagccgtc gcatgtctga ttgctcacca ggtaaatgtc
     6121 gctaatgttt tccaacgcga gaaggtgttg agcgcggagc tgagtgacgt gacaacatgg
     6181 gtatgcccaa ttgccccatg ttgggaggac gaaaatggtg acaagacaga tggccagaaa
     6241 tacaccaaca gcacgcatga tgtctactgg ggatttattc tttagtgcgg gggaatacac
     6301 ggcttttaat acgattgagg gcgtctccta acaagttaca tcactcctgc ccttcctcac
     6361 cctcatctcc atcacctcct tcatctccgt catctccgtc atcaccctcc gcggcagccc
     6421 cttccaccat aggtggaaac cagggaggca aatctactcc atcgtcaaag ctgcacacag
     6481 tcaccctgat attgcaggta ggagcgggct ttgtcataac aaggtcctta atcgcatcct
     6541 tcaaaacctc agcaaatata tgagtttgta aaaagaccat gaaataacag acaatggact
     6601 cccttagcgg gccaggttgt gggccgggtc caggggccat tccaaagggg agacgactca
     6661 atggtgtaag acgacattgt ggaatagcaa gggcagttcc tcgccttagg ttgtaaaggg
     6721 aggtcttact acctccatat acgaacacac cggcgaccca agttccttcg tcggtagtcc
     6781 tttctacgtg actcctagcc aggagagctc ttaaaccttc tgcaatgttc tcaaatttcg
     6841 ggttggaacc tccttgacca cgatgctttc caaaccaccc tccttttttg cgcctgcctc
     6901 catcaccctg accccggggt ccagtgcttg ggccttctcc tgggtcatct gcggggccct
     6961 gctctatcgc tcccgggggc acgtcaggct caccatctgg gccaccttct tggtggtatt
     7021 caaaataatc ggcttcccct acagggtgga aaaatggcct tctacctgga gggggcctgc
     7081 gcggtggaga cccggatgat gatgactgac tactgggact cctgggcctc ttttctccac
     7141 gtccacgacc tctccccctg gctctttcac gacttccccc cctggctctt tcacgtcctc
     7201 taccccggcg gcctccacta cctcctcgac cccggcctcc actacctcct cgaccccggc
     7261 ctccactgcc tcctcgaccc cggcctccac ctcctgctcc tgcccctcct gctcctgccc
     7321 ctcctcctgc tcctgcccct cctgcccctc ctgctcctgc ccctcctgcc cctcctgctc
     7381 ctgcccctcc tgcccctcct gctcctgccc ctcctgcccc tcctcctgct cctgcccctc
     7441 ctgcccctcc tcctgctcct gcccctcctg cccctcctgc tcctgcccct cctgcccctc
     7501 ctgctcctgc ccctcctgcc cctcctgctc ctgcccctcc tgctcctgcc cctcctgctc
     7561 ctgcccctcc tgctcctgcc cctcctgccc ctcctgcccc tcctcctgct cctgcccctc
     7621 ctgctcctgc ccctcctgcc cctcctgccc ctcctgctcc tgcccctcct cctgctcctg
     7681 cccctcctgc ccctcctgcc cctcctcctg ctcctgcccc tcctgcccct cctcctgctc
     7741 ctgcccctcc tcctgctcct gcccctcctg cccctcctgc ccctcctcct gctcctgccc
     7801 ctcctgcccc tcctcctgct cctgcccctc ctcctgctcc tgcccctcct gcccctcctg
     7861 cccctcctcc tgctcctgcc cctcctcctg ctcctgcccc tcctgcccct cctgcccctc
     7921 ctgcccctcc tcctgctcct gcccctcctc ctgctcctgc ccctcctgct cctgcccctc
     7981 ccgctcctgc tcctgctcct gttccaccgt gggtcccttt gcagccaatg caacttggac
     8041 gtttttgggg tctccggaca ccatctctat gtcttggccc tgatcctgag ccgcccgggg
     8101 ctcctggtct tccgcctcct cgtcctcgtc ctcttccccg tcctcgtcca tggttatcac
     8161 cccctcttct ttgaggtcca ctgccgccgg agccttctgg tccagatgtg tctcccttct
     8221 ctcctaggcc atttccaggt cctgtacctg gcccctcgtc agacatgatt cacactaaaa
     8281 gagatcaata gacatcttta ttagacgacg ctcagtgaat acagggagtg cagactcctg
     8341 ccccctccaa cagccccccc accctcatcc ccttcatggt cgctgtcaga cagatccagg
     8401 tctgaaaatt ccccatcctc cgaaccatcc tcgtcctcat caccaattac tcgcagcccg
     8461 gaaaactccc gctgaacatc ctcaagattt gcgtcctgag cctcaagcca ggcctcaaat
     8521 tcctcgtccc cctttttgct ggacggtagg gatggggatt ctcgggaccc ctcctcttcc
     8581 tcttcaaggt caccagacag agatgctact ggggcaacgg aagaaaagct gggtgcggcc
     8641 tgtgaggatc agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg
     8701 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     8761 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     8821 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     8881 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     8941 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     9001 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     9061 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     9121 cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     9181 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     9241 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     9301 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     9361 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     9421 acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     9481 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     9541 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     9601 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     9661 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     9721 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     9781 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     9841 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     9901 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     9961 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
    10021 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
    10081 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
    10141 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
    10201 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
    10261 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
    10321 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
    10381 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
    10441 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
    10501 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctgcgccg
    10561 cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc
    10621 attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc
    10681 gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac
    10741 gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg
    10801 ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg
    10861 gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac
    10921 agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg
    10981 aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc
    11041 atgccctgct tcatccccgt ggcccgttgc tcgcgtttgc tggcggtgtc cccggaagaa
    11101 atatatttgc atgtctttag ttctatgatg acacaaaccc cgcccagcgt cttgtcattg
    11161 gcgaattcga acacgcagat gcagtcgggg cggcgcggtc ccaggtccac ttcgcatatt
    11221 aaggtgacgc gtgtggcctc gaacaccgag cgaccctgca gcgacccgct taacagcgtc
    11281 aacagcgtgc cgcagatccc gggcaatgag atatgaaaaa gcctgaactc accgcgacgt
    11341 ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg
    11401 agggcgaaga atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg
    11461 taaatagctg cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatcgg
    11521 ccgcgctccc gattccggaa gtgcttgaca ttggggaatt cagcgagagc ctgacctatt
    11581 gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg
    11641 ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat cttagccaga
    11701 cgagcgggtt cggcccattc ggaccgcaag gaatcggtca atacactaca tggcgtgatt
    11761 tcatatgcgc gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg
    11821 tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct ttgggccgag gactgccccg
    11881 aagtccggca cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg gacaatggcc
    11941 gcataacagc ggtcattgac tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg
    12001 ccaacatctt cttctggagg ccgtggttgg cttgtatgga gcagcagacg cgctacttcg
    12061 agcggaggca tccggagctt gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg
    12121 gtcttgacca actctatcag agcttggttg acggcaattt cgatgatgca gcttgggcgc
    12181 agggtcgatg cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt acacaaatcg
    12241 cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc gatagtggaa
    12301 accgacgccc cagcactcgt ccgagggcaa aggaataggg gagatggggg aggctaactg
    12361 aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga
    12421 ataaaacgca cgggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg
    12481 cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt
    12541 tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg
    12601 gcaggccctg ccatagccac tggccccgtg ggttagggac ggggtccccc atggggaatg
    12661 gtttatggtt cgtgggggtt attattttgg gcgttgcgtg gggtctggtc cacgactgga
    12721 ctgagcagac agacccatgg tttttggatg gcctgggcat ggaccgcatg tactggcgcg
    12781 acacgaacac cgggcgtctg tggctgccaa acacccccga cccccaaaaa ccaccgcgcg
    12841 gatttctggc gtgccaagct agtcgaccaa ttctcatgtt tgacagctta tcatcgcaga
    12901 tccgggcaac gttgttgcat tgctgcaggc gcagaactgg taggtatgga agatctatac
    12961 attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat aaatcaatat
    13021 tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt atattggctc
    13081 atgtccaata tgaccgccat gttgacattg attattgact agttattaat agtaatcaat
    13141 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
    13201 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
    13261 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
    13321 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc ctattgacgt
    13381 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac gggactttcc
    13441 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
    13501 gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
    13561 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
    13621 taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
    13681 cagagctcgt ttagtgaacc gtcagatctc tagaagctgg gtaccagct
//
