LOCUS       sequence-263287-        2856 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Confers plant resistance to kanamycin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Sauret-Gueto S, Frangedakis E, Silvestri L, Rebmann M, Tomaselli M, 
            Markel K, Delmans M, West A, Patron NJ, Haseloff J
  TITLE     Systematic tools for reprogramming plant gene expression in a simple
            model, Marchantia polymorpha.
  JOURNAL   ACS Synth Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511.
  PUBMED    32163700
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511."
COMMENT     Sequence Label: CDS_nptII
FEATURES             Location/Qualifiers
     source          1..2856
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     162..180
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(184..227)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     CDS             244..1041
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(301..320)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     911..930
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      1059..1116
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1140..1157)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1311..1899)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1391..1410)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2082..2176)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2197..2856)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2771..2790
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct
       61 tatttcatta tggtgaaagt tggaacctct tacgtgcccg atcaactcga gtgccacctg
      121 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
      181 agaatttcag ataaaaaaaa tccttagctt tcgctaagga tgatttctgg aattcggtct
      241 caaatgggga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
      301 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgtttcgg
      361 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
      421 gaactccagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
      481 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
      541 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
      601 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
      661 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
      721 gacgaggagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg
      781 cccgacggcg aggatctcgt cgtgacacat ggcgatgcct gcttgccgaa tatcatggtg
      841 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
      901 caggacatag cgttggctac ccgtgatatt gctgaggagc ttggcggcga atgggctgac
      961 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
     1021 cttcttgacg agttcttctg agcttcgaga ccctgcagtc cggcaaaaaa gggcaaggtg
     1081 tcaccaccct gccctttttc tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc
     1141 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     1201 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     1261 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg
     1321 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     1381 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     1441 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     1501 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     1561 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     1621 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     1681 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     1741 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     1801 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     1861 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     1921 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     1981 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     2041 agtatatatg agtaaacttg gtctgacagc tcgaggcttg gattctcacc aataaaaaac
     2101 gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga
     2161 tctatcaaca ggagtccaag cgagctcgat atcaaattac gccccgccct gccactcatc
     2221 gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg
     2281 atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat
     2341 ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa
     2401 actcacccag ggattggctg agacgaaaaa catattctca ataaaccctt tagggaaata
     2461 ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa
     2521 atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt
     2581 gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg ccatacgaaa
     2641 ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg
     2701 cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata
     2761 ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat
     2821 atcaacggtg gtatatccag tgattttttt ctccat
//
