LOCUS       sequence-263302-        7727 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses 3xMyc-HPS4 construct in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7727)
  AUTHORS   Martina JA, Moriyama K, Bonifacino JS
  TITLE     BLOC-3, a protein complex containing the Hermansky-Pudlak syndrome 
            gene products HPS1 and HPS4.
  JOURNAL   J Biol Chem. 2003 Aug 1;278(31):29376-84. doi: 
            10.1074/jbc.M301294200. Epub 2003 May 19.
  PUBMED    12756248
REFERENCE   2  (bases 1 to 7727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M301294200"; journalName: "J Biol Chem"; date: 
            "2003-08-1- 1"; volume: "278"; issue: "31"; pages: "29376-84"
COMMENT     Sequence Label: pCI-Myc3-HPS4
FEATURES             Location/Qualifiers
     source          1..7727
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        57..436
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        437..648
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     599..619
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     645..669
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          809..941
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     924..943
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        986..1004
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1016..1025
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1025..1054
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1058..1087
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1094..1123
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    3350..3471
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3361..3380)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3415..3434
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      3657..4112
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3744..3763)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3954..3975
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4229..4586
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4229..4420
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4252..4272)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4437..4572
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4637..5431
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4691..4710)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5301..5320
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5495..5543
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(5584..5603)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5703..5725
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5763..5781)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5849..5953
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5954..6022,6023..6814)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6172..6191)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6985..7573
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7474..7493
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tctatatcat aatatgtaca tttatattgg ctcatgtcca atatgaccgc catgttggca
       61 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      121 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      181 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      241 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      301 gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      361 ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct acgtattagt
      421 catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg gatagcggtt
      481 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
      541 ccaaaatcaa cgggactttc caaaatgtcg taacaactgc gatcgcccgc cccgttgacg
      601 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
      661 cgtcagatca ctagaagctt tattgcggta gtttatcaca gttaaattgc taacgcagtc
      721 agtgcttctg acacaacagt ctcgaactta agctgcagtg actctcttaa ggtagccttg
      781 cagaagttgg tcgtgaggca ctgggcaggt aagtatcaag gttacaagac aggtttaagg
      841 agaccaatag aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
      901 tattggtctt actgacatcc actttgcctt tctctccaca ggtgtccact cccagttcaa
      961 ttacagctct taaggctaga gtacttaata cgactcacta taggctagcc tcgaggccac
     1021 catggagcag aagctgatct ccgaagagga cctcggcgaa cagaaactca tctctgagga
     1081 agatctgatg ggggaacaga agctgatttc cgaggaagac ctggaattca tggccacctc
     1141 tacctccaca gaggcaaagt cagcctcgtg gtggaattat ttttttcttt atgatggttc
     1201 caaggtaaag gaagaaggcg atccaacaag agctggcatt tgttactttt atccttccca
     1261 gaccctgcta gaccaacagg agttgctttg tggacagatt gctggagttg tccgctgtgt
     1321 ttctgacatt tctgactctc ctcctactct tgttcgtctg agaaaactga agtttgccat
     1381 aaaagttgat ggagattacc tttgggtgct gggctgtgct gtggagctcc ctgatgtcag
     1441 ctgcaagcgg tttctggatc agctagttgg attctttaat ttttacaatg gacctgtttc
     1501 cctagcttat gagaactgtt ctcaggaaga actgagcacg gagtgggaca ccttcatcga
     1561 gcaaattctg aaaaacacca gtgatctgca taagattttc aattccctct ggaacttgga
     1621 ccaaactaaa gtggagcccc tgttgttgct gaaggcagcc cgcattctgc agacctgcca
     1681 gcgctcgcct cacattctcg ctggctgcat cctctataaa ggactgattg tcagcaccca
     1741 actcccgccc tccctcaccg ccaaggtcct gcttcaccga acagcacctc aggagcagag
     1801 actccctacg ggaggggatg ccccgcagga acatggagcg gcattgcccc cgaatgtcca
     1861 gattatccct gtttttgtga ccaaagagga agccattagt ctccacgagt tcccggtgga
     1921 acagatgaca aggtctctag catctccagc aggactccag gatggttcag cccagcacca
     1981 tccaaagggt gggagcacat ctgccctgaa agaaaacgcc actggccatg tggaatccat
     2041 ggcctggacc accccagatc ccacatcccc tgacgaagct tgtccagatg gcaggaagga
     2101 gaacggatgc ttgtctggcc atgatctgga gagcatcagg cccgcaggac tgcacaactc
     2161 tgccaggggt gaggttcttg gcctcagctc ctccctgggg aaggaactag tctttctcca
     2221 agaagaactc gacttgtctg aaatccacat tccagaggct caggaagtgg aaatggcctc
     2281 aggtcatttt gccttcctac atgtgcctgt tccagatggc agggctcctt actgcaaggc
     2341 atctctcagc gcctccagca gcctggaacc cacgcctcct gaggacacag ccatcagcag
     2401 cttgcgccct ccctctgctc ctgagatgct gacccagcat ggagcccaag agcaggtcga
     2461 agaccatcct ggccatagca gccaagcccc cattcccaga gcagaccctc tccccagaag
     2521 gacccgcagg cccttgttat tgcctcgctt agatccagga cagagaggaa acaagcttcc
     2581 cacgggggaa caaggcctgg atgaggatgt tgatggggtc tgtgaaagcc acgcagcccc
     2641 tggtctggaa tgcagttcag gctcagcaaa ctgtcagggt gctggcccct ctgcagatgg
     2701 aatcagctcc aggctgacac cagcagagtc ctgcatgggg ctcgtgagga tgaatctcta
     2761 cactcactgc gtcaaagggc tgatgctgtc cctgctggct gaggagccgc tgctgggaga
     2821 cagcgcagcc atagaggaag tgtaccacag cagcctggct tcactgaatg ggctggaagt
     2881 ccacctgaaa gagacgctgc ccagggatga ggcagcctcc acgagcagca cctacaactt
     2941 cacatattac gaccgcattc agagcttgct gatggcaaac ctgccgcagg tggccacccc
     3001 gcatgatcgc cgcttcctcc aggccgtcag cctgatgcat agcgaatttg cccagctgcc
     3061 cgcgctttat gaaatgactg tcagaaatgc ctccacggct gtgtacgcct gttgcaaccc
     3121 catccaggag acatatttcc agcagctggc acctgcagca cggagctccg gcttcccaaa
     3181 ccctcaggat ggcgccttca gcctctccgg caaagcaaag cagaagctgc tgaagcacgg
     3241 ggtgaacttg ctctgaccgg gggaagggcg aattcacgcg tggtacctct agagtcgacc
     3301 cgggcggccg cttcccttta gtgagggtta atgcttcgag cagacatgat aagatacatt
     3361 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
     3421 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
     3481 aattgcattc attttatgtt tcaggttcag ggggagatgt gggaggtttt ttaaagcaag
     3541 taaaacctct acaaatgtgg taaaatccga taaggatcga tccgggctgg cgtaatagcg
     3601 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggacgc
     3661 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     3721 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     3781 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     3841 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     3901 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     3961 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     4021 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     4081 gaattttaac aaaatattaa cgcttacaat ttcctgatgc ggtattttct ccttacgcat
     4141 ctgtgcggta tttcacaccg catacgcgga tctgcgcagc accatggcct gaaataacct
     4201 ctgaaagagg aacttggtta ggtaccttct gaggcggaaa gaaccagctg tggaatgtgt
     4261 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     4321 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     4381 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     4441 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     4501 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     4561 tttttggagg cctaggcttt tgcaaaaagc ttgattcttc tgacacaaca gtctcgaact
     4621 taaggctaga gccaccatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     4681 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     4741 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     4801 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     4861 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     4921 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     4981 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     5041 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     5101 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     5161 ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     5221 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     5281 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     5341 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     5401 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac
     5461 caagcgacgc ccaacctgcc atcacgatgg ccgcaataaa atatctttat tttcattaca
     5521 tctgtgtgtt ggttttttgt gtgaatcgat agcgataagg atccgcgtat ggtgcactct
     5581 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
     5641 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     5701 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa
     5761 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     5821 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     5881 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     5941 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     6001 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     6061 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     6121 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     6181 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     6241 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     6301 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     6361 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     6421 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     6481 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     6541 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     6601 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     6661 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     6721 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     6781 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     6841 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     6901 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     6961 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     7021 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     7081 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     7141 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     7201 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     7261 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     7321 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     7381 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     7441 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     7501 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     7561 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     7621 ttttgctcac atggctcgac agatcttcaa tattggccat tagccatatt attcattggt
     7681 tatatagcat aaatcaatat tggctattgg ccattgcata cgttgta
//
