LOCUS       sequence-263303-        7682 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses 3xHA-HPS1 construct in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7682)
  AUTHORS   Martina JA, Moriyama K, Bonifacino JS
  TITLE     BLOC-3, a protein complex containing the Hermansky-Pudlak syndrome 
            gene products HPS1 and HPS4.
  JOURNAL   J Biol Chem. 2003 Aug 1;278(31):29376-84. doi: 
            10.1074/jbc.M301294200. Epub 2003 May 19.
  PUBMED    12756248
REFERENCE   2  (bases 1 to 7682)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M301294200"; journalName: "J Biol Chem"; date: 
            "2003-08-1- 1"; volume: "278"; issue: "31"; pages: "29376-84"
COMMENT     Sequence Label: pCI-HA3-HPS1
FEATURES             Location/Qualifiers
     source          1..7682
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        168..547
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        548..759
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     710..730
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     756..780
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          920..1052
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1035..1054
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1097..1115
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1137..1163
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1167..1193
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1200..1226
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    3416..3537
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3427..3446)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3481..3500
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      3723..4178
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3810..3829)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4020..4041
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4295..4652
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4295..4486
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4318..4338)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4503..4638
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4703..5497
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4757..4776)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5367..5386
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5561..5609
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(5650..5669)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5769..5791
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5829..5847)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5915..6019
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6020..6088,6089..6880)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6238..6257)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7051..7639
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7540..7559
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggccttttgc tcacatggct cgacagatct tcaatattgg ccattagcca tattattcat
       61 tggttatata gcataaatca atattggcta ttggccattg catacgttgt atctatatca
      121 taatatgtac atttatattg gctcatgtcc aatatgaccg ccatgttggc attgattatt
      181 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
      241 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
      301 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
      361 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
      421 gccaagtccg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
      481 gtacatgacc ttacgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
      541 taccatggtg atgcggtttt ggcagtacac caatgggcgt ggatagcggt ttgactcacg
      601 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
      661 acgggacttt ccaaaatgtc gtaacaactg cgatcgcccg ccccgttgac gcaaatgggc
      721 ggtaggcgtg tacggtggga ggtctatata agcagagctc gtttagtgaa ccgtcagatc
      781 actagaagct ttattgcggt agtttatcac agttaaattg ctaacgcagt cagtgcttct
      841 gacacaacag tctcgaactt aagctgcagt gactctctta aggtagcctt gcagaagttg
      901 gtcgtgaggc actgggcagg taagtatcaa ggttacaaga caggtttaag gagaccaata
      961 gaaactgggc ttgtcgagac agagaagact cttgcgtttc tgataggcac ctattggtct
     1021 tactgacatc cactttgcct ttctctccac aggtgtccac tcccagttca attacagctc
     1081 ttaaggctag agtacttaat acgactcact ataggctagc ctcgagcgcc accatgtacc
     1141 cgtacgatgt tcctgactat gcgggctatc cctatgacgt cccggactat gccatgggct
     1201 acccttacga cgttccagat tacgctaagc ttatgaagtg cgtcttggtg gccactgagg
     1261 gcgcagaggt cctcttctac tggacagatc aggagtttga agagagtctc cggctgaagt
     1321 tcgggcagtc agagaatgag gaagaagagc tccctgccct ggaggaccag ctcagcaccc
     1381 tcctagcccc ggtcatcatc tcctccatga cgatgctgga gaagctctcg gacacctaca
     1441 cctgcttctc cacggaaaat ggcaacttcc tgtatgtcct tcacctgttt ggagaatgcc
     1501 tgttcattgc catcaatggt gaccacaccg agagcgaggg ggacctgcgg cggaagctgt
     1561 atgtgctcaa gtacctgttt gaagtgcact ttgggctggt gactgtggac ggtcatctta
     1621 tccgaaagga gctgcggccc ccagacctgg cgcagcgtgt ccagctgtgg gagcacttcc
     1681 agagcctgct gtggacctac agccgcctgc gggagcagga gcagtgcttc gccgtggagg
     1741 ccctggagcg actgattcac ccccagctct gtgagctgtg catagaggcg ctggagcggc
     1801 acgtcatcca ggctgtcaac accagccccg agcggggagg cgaggaggcc ctgcatgcct
     1861 tcctgctcgt gcactccaag ctgctggcat tctactctag ccacagtgcc agctccctgc
     1921 gcccggccga cctgcttgcc ctcatcctcc tggttcagga cctctacccc agcgagagca
     1981 cagcagagga cgacattcag ccttccccgc ggagggcccg gagcagccag aacatccccg
     2041 tgcagcaggc ctggagccct cactccacgg gcccaactgg ggggagctct gcagagacgg
     2101 agacagacag cttctccctc cctgaggagt acttcacacc agctccttcc cctggcgatc
     2161 agagctcagg tagcaccatc tggctggagg ggggcacccc ccccatggat gcccttcaga
     2221 tagcagagga caccctccaa acactggttc cccactgccc tgtgccttcc ggccccagaa
     2281 ggatcttcct ggatgccaac gtgaaggaaa gctactgccc cctagtgccc cacaccatgt
     2341 actgcctgcc cctgtggcag ggcatcaacc tggtgctcct gaccaggagc cccagcgcgc
     2401 ccctggccct ggttctgtcc cagctgatgg atggcttctc catgctggag aagaagctga
     2461 aggaagggcc ggagcccggg gcctccctgc gctcccagcc cctcgtggga gacctgcgcc
     2521 agaggatgga caagtttgtc aagaatcgag gggcacagga gattcagagc acctggctgg
     2581 agtttaaggc caaggctttc tccaaaagtg agcccggatc ctcctgggag ctgctccagg
     2641 catgtgggaa gctgaagcgg cagctctgcg ccatctaccg gctgaacttt ctgaccacag
     2701 cccccagcag gggaggccca cacctgcccc agcacctgca ggaccaagtg cagaggctca
     2761 tgcgggagaa gctgacggac tggaaggact tcttgctggt gaagagcagg aggaacatca
     2821 ccatggtgtc ctacctagaa gacttcccag gcttggtgca cttcatctat gtggaccgca
     2881 ccactgggca gatggtggcg ccttccctca actgcagtca aaagacctcg tcggagttgg
     2941 gcaaggggcc gctggctgcc tttgtcaaaa ctaaggtctg gtctctgatc cagctggcgc
     3001 gcagatacct gcagaagggc tacaccacgc tgctgttcca ggagggggat ttctactgct
     3061 cctacttcct gtggttcgag aatgacatgg ggtacaaact ccagatgatc gaggtgcccg
     3121 tcctctccga cgactcagtg cctatcggca tgctgggagg agactactac aggaagctcc
     3181 tgcgctacta cagcaagaac cgcccaaccg aggctgtcag gtgctacgag ctgctggccc
     3241 tgcacctgtc tgtcatcccc actgacctgc tggtgcagca ggccggccag ctggcccggc
     3301 gcctctggga ggcctcccgt atccccctgc tctaggaatt cacgcgtggt acctctagag
     3361 tcgacccggg cggccgcttc cctttagtga gggttaatgc ttcgagcaga catgataaga
     3421 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     3481 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
     3541 aacaacaatt gcattcattt tatgtttcag gttcaggggg agatgtggga ggttttttaa
     3601 agcaagtaaa acctctacaa atgtggtaaa atccgataag gatcgatccg ggctggcgta
     3661 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     3721 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     3781 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     3841 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     3901 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     3961 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     4021 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     4081 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     4141 taacgcgaat tttaacaaaa tattaacgct tacaatttcc tgatgcggta ttttctcctt
     4201 acgcatctgt gcggtatttc acaccgcata cgcggatctg cgcagcacca tggcctgaaa
     4261 taacctctga aagaggaact tggttaggta ccttctgagg cggaaagaac cagctgtgga
     4321 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     4381 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     4441 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     4501 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     4561 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     4621 gaggcttttt tggaggccta ggcttttgca aaaagcttga ttcttctgac acaacagtct
     4681 cgaacttaag gctagagcca ccatgattga acaagatgga ttgcacgcag gttctccggc
     4741 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
     4801 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
     4861 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac
     4921 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
     4981 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
     5041 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
     5101 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
     5161 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
     5221 gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
     5281 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
     5341 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
     5401 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
     5461 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg
     5521 accgaccaag cgacgcccaa cctgccatca cgatggccgc aataaaatat ctttattttc
     5581 attacatctg tgtgttggtt ttttgtgtga atcgatagcg ataaggatcc gcgtatggtg
     5641 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
     5701 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     5761 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     5821 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     5881 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     5941 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     6001 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     6061 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     6121 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     6181 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     6241 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     6301 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     6361 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     6421 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     6481 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     6541 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     6601 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     6661 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     6721 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     6781 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     6841 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     6901 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     6961 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     7021 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     7081 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     7141 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     7201 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     7261 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     7321 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     7381 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     7441 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     7501 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     7561 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     7621 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     7681 ct
//
