LOCUS       sequence-263307-       10368 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression plasmid for multivalent scaffold of 
            (SUMO)10-(SIM)5 biomolecular condensates to recruit SIM-containing 
            client proteins..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10368)
  AUTHORS   Banani SF, Rice AM, Peeples WB, Lin Y, Jain S, Parker R, Rosen MK
  TITLE     Compositional Control of Phase-Separated Cellular Bodies.
  JOURNAL   Cell. 2016 Jul 28;166(3):651-663. doi: 10.1016/j.cell.2016.06.010. 
            Epub 2016 Jun 30.
  PUBMED    27374333
REFERENCE   2  (bases 1 to 10368)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.06.010"; journalName: "Cell"; date: 
            "2016-07-28- 28"; volume: "166"; issue: "3"; pages: "651-663"
COMMENT     Sequence Label: (SUMO)10-(SIM)5
FEATURES             Location/Qualifiers
     source          1..10368
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1..6,7..22,23..29)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    37..53
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     42..64
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             123..1223
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKTEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     regulatory      1080..1089
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     1197..1220
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             1272..1283
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             1296..1316
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             4980..5000
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             5001..5018
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5031..5048)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5031..5047)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5040..5062)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5226..5243)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      5396..5482
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5574..5601
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        5620..5711
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5712..5780,5781..6572)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5930..5949)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(6614..7127)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6809..6830)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7021..7040
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      7238..7826
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7727..7746
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7980..7997
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8103..8122)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8263..8285
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8279..8470)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        8966..9043
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             9044..10126
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(9063..9082)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 ttgacaatta atcatcggct cgtataatgt gtggaattgt gagcggataa caatttcaca
       61 caggaaacag ccagtccgtt taggtgtttt cacgagcact tcaccaacaa ggaccataga
      121 ttatgaaaac tgaagaaggt aaactggtaa tctggattaa cggcgataaa ggctataacg
      181 gtctcgctga agtcggtaag aaattcgaga aagataccgg aattaaagtc accgttgagc
      241 atccggataa actggaagag aaattcccac aggttgcggc aactggcgat ggccctgaca
      301 ttatcttctg ggcacacgac cgctttggtg gctacgctca atctggcctg ttggctgaaa
      361 tcaccccgga caaagcgttc caggacaagc tgtatccgtt tacctgggat gccgtacgtt
      421 acaacggcaa gctgattgct tacccgatcg ctgttgaagc gttatcgctg atttataaca
      481 aagatctgct gccgaacccg ccaaaaacct gggaagagat cccggcgctg gataaagaac
      541 tgaaagcgaa aggtaagagc gcgctgatgt tcaacctgca agaaccgtac ttcacctggc
      601 cgctgattgc tgctgacggg ggttatgcgt tcaagtatga aaacggcaag tacgacatta
      661 aagacgtggg cgtggataac gctggcgcga aagcgggtct gaccttcctg gttgacctga
      721 ttaaaaacaa acacatgaat gcagacaccg attactccat cgcagaagct gcctttaata
      781 aaggcgaaac agcgatgacc atcaacggcc cgtgggcatg gtccaacatc gacaccagca
      841 aagtgaatta tggtgtaacg gtactgccga ccttcaaggg tcaaccatcc aaaccgttcg
      901 ttggcgtgct gagcgcaggt attaacgccg ccagtccgaa caaagagctg gcaaaagagt
      961 tcctcgaaaa ctatctgctg actgatgaag gtctggaagc ggttaataaa gacaaaccgc
     1021 tgggtgccgt agcgctgaag tcttacgagg aagagttggc gaaagatcca cgtattgccg
     1081 ccaccatgga aaacgcccag aaaggtgaaa tcatgccgaa catcccgcag atgtccgctt
     1141 tctggtatgc cgtgcgtact gcggtgatca acgccgccag cggtcgtcag actgtcgatg
     1201 aagccctgaa agacgcgcag actaattcga gctcgaacaa caacaacaat aacaataaca
     1261 acaacctcgg gatcgaggga aggatttcag aatttgaaaa cctgtatttt cagggccata
     1321 tgggtgggag ttggggaggg tctatgtcag aagagaagcc taaggagggc gtgaaaaccg
     1381 agaatgacca cattaacttg aaggttgctg gacaggatgg gtccgtggtt cagttcaaaa
     1441 ttaaacgcca cacaccgttg tcgaaactga tgaaagcgta cagcgaacgc cagggtcttt
     1501 ctatgcgtca gattcgcttc cgctttgatg gtcaaccgat taatgaaacg gacacgccgg
     1561 cacagctgga gatggaggat gaagacacta tcgacgtatt tcagcagcag accgtcgtag
     1621 ggggctccgg cggaagcggg gggtcgggag gttccatgag cgaagagaaa ccaaaagaag
     1681 gagttaaaac cgaaaacgac catatcaacc tgaaggtcgc cggtcaggac ggctcagtcg
     1741 tgcagttcaa gattaaacgt cataccccat taagtaagct gatgaaggcg tatagtgagc
     1801 gccaaggctt gtcgatgcgc cagattcgtt ttcgctttga tggtcagcca atcaacgaaa
     1861 ccgacacccc ggcgcagctg gaaatggaag atgaagatac catcgatgtg ttccaacagc
     1921 aaacggttgt tggcggaagc ggcgggagcg gtggtagcgg gggttctatg tccgaagaaa
     1981 aaccgaaaga gggtgtgaag actgaaaacg accacattaa tctgaaggtg gcgggccagg
     2041 acggttccgt tgttcagttt aaaatcaaac gtcacacccc gctgtcgaaa ctgatgaaag
     2101 cctatagcga acgtcaaggg ctgagcatgc gtcaaattcg ttttcgcttt gatggtcagc
     2161 caatcaacga aaccgataca ccggcgcagc tggagatgga agacgaggac accattgacg
     2221 tcttccagca acaaactgta gtgggtggct ccggtggctc tggtgggtct ggtggctcga
     2281 tgagtgagga aaaacccaaa gaaggcgtca agactgagaa cgatcatatt aacctgaaag
     2341 tcgcgggtca agatggttca gtggtgcagt tcaaaattaa acgccacacc ccgctgtcaa
     2401 agctgatgaa agcctatagt gagcgtcaag gtctgtcgat gcgccagatc cgctttcgct
     2461 ttgatggtca gccaatcaac gagacagaca ctccggcaca actggagatg gaagatgaag
     2521 acaccatcga tgtttttcaa cagcagaccg tggtgggagg gagcggcggt agcgggggat
     2581 caggtggtag tatgtctgag gaaaagccga aggaaggggt aaagaccgaa aatgatcaca
     2641 ttaatctgaa agttgcgggc caagatggta gtgtagtgca atttaaaatc aagcgtcaca
     2701 ccccgttatc taaactgatg aaagcatata gcgaacgcca aggcctgtct atgcgccaga
     2761 ttcgctttcg ctttgacggg caaccaatta atgaaacgga caccccggca caactggaaa
     2821 tggaagacga ggatactatt gacgtgtttc agcaacagac agtggtcggc ggctccggtg
     2881 gatctggtgg gagttgggga gggtctatgt cagaagagaa gcctaaggag ggcgtgaaaa
     2941 ccgagaatga ccacattaac ttgaaggttg ctggacagga tgggtccgtg gttcagttca
     3001 aaattaaacg ccacacaccg ttgtcgaaac tgatgaaagc gtacagcgaa cgccagggtc
     3061 tttctatgcg tcagattcgc ttccgctttg atggtcaacc gattaatgaa acggacacgc
     3121 cggcacagct ggagatggag gatgaagaca ctatcgacgt atttcagcag cagaccgtcg
     3181 tagggggctc cggcggaagc ggggggtcgg gaggttccat gagcgaagag aaaccaaaag
     3241 aaggagttaa aaccgaaaac gaccatatca acctgaaggt cgccggtcag gacggctcag
     3301 tcgtgcagtt caagattaaa cgtcataccc cattaagtaa gctgatgaag gcgtatagtg
     3361 agcgccaagg cttgtcgatg cgccagattc gttttcgctt tgatggtcag ccaatcaacg
     3421 aaaccgacac cccggcgcag ctggaaatgg aagatgaaga taccatcgat gtgttccaac
     3481 agcaaacggt tgttggcgga agcggcggga gcggtggtag cgggggttct atgtccgaag
     3541 aaaaaccgaa agagggtgtg aagactgaaa acgaccacat taatctgaag gtggcgggcc
     3601 aggacggttc cgttgttcag tttaaaatca aacgtcacac cccgctgtcg aaactgatga
     3661 aagcctatag cgaacgtcaa gggctgagca tgcgtcaaat tcgttttcgc tttgatggtc
     3721 agccaatcaa cgaaaccgat acaccggcgc agctggagat ggaagacgag gacaccattg
     3781 acgtcttcca gcaacaaact gtagtgggtg gctccggtgg ctctggtggg tctggtggct
     3841 cgatgagtga ggaaaaaccc aaagaaggcg tcaagactga gaacgatcat attaacctga
     3901 aagtcgcggg tcaagatggt tcagtggtgc agttcaaaat taaacgccac accccgctgt
     3961 caaagctgat gaaagcctat agtgagcgtc aaggtctgtc gatgcgccag atccgctttc
     4021 gctttgatgg tcagccaatc aacgagacag acactccggc acaactggag atggaagatg
     4081 aagacaccat cgatgttttt caacagcaga ccgtggtggg agggagcggc ggtagcgggg
     4141 gatcaggtgg tagtatgtct gaggaaaagc cgaaggaagg ggtaaagacc gaaaatgatc
     4201 acattaatct gaaagttgcg ggccaagatg gtagtgtagt gcaatttaaa atcaagcgtc
     4261 acaccccgtt atctaaactg atgaaagcat atagcgaacg ccaaggcctg tctatgcgcc
     4321 agattcgctt tcgctttgac gggcaaccaa ttaatgaaac ggacaccccg gcacaactgg
     4381 aaatggaaga cgaggatact attgacgtgt ttcagcaaca gacagtggtc ggcggctccg
     4441 gtggatctgg tggctcttgg ggtgggagca aagtagatgt gatcgacttg accatcgaaa
     4501 gcagcagcga cgaagaagaa gatccgcctg ccaaacgcgg cggtagcggt ggctctggcg
     4561 ggagcggcgg ctcgaaagtt gatgtcattg accttacaat tgaatcttcg agcgatgaag
     4621 aggaagatcc tccagcgaaa cgtggcggct cgggaggttc gggcggcagc ggcggaagca
     4681 aagtcgatgt aattgactta acgatcgaat ctagcagcga tgaagaagaa gatccaccgg
     4741 ctaaacgtgg gggctcaggg ggttctggcg gttcaggcgg cagtaaagtt gacgtgattg
     4801 acctcaccat cgaaagcagt agcgacgagg aagaagatcc accagcaaaa cgcggaggga
     4861 gcgggggcag cggcggcagc ggcggatcta aagtggacgt tatcgatctg acgatcgaaa
     4921 gcagtagcga tgaggaagag gacccgccgg cgaaacgggg cggctcgtgc gggggatccg
     4981 aaaacctgta ttttcagggc catcatcatc atcatcactg aaagcttggc actggccgtc
     5041 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     5101 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     5161 cagttgcgca gcctgaatgg cgaatggcag cttggctgtt ttggcggatg agataagatt
     5221 ttcagcctga tacagattaa atcagaacgc agaagcggtc tgataaaaca gaatttgcct
     5281 ggcggcagta gcgcggtggt cccacctgac cccatgccga actcagaagt gaaacgccgt
     5341 agcgccgatg gtagtgtggg gtctccccat gcgagagtag ggaactgcca ggcatcaaat
     5401 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
     5461 cgctctcctg agtaggacaa atccgccggg agcggatttg aacgttgcga agcaacggcc
     5521 cggagggtgg cgggcaggac gcccgccata aactgccagg catcaaatta agcagaaggc
     5581 catcctgacg gatggccttt ttgcgtttct acaaactctt ttgtttattt ttctaaatac
     5641 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     5701 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     5761 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     5821 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     5881 gttttcgccc cgaagaacgt ttcccaatga tgagcacttt taaagttctg ctatgtggcg
     5941 cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     6001 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     6061 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     6121 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     6181 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     6241 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     6301 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     6361 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     6421 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     6481 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     6541 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     6601 tttagattga tttaccccgg ttgataatca gaaaagcccc aaaaacagga agattgtata
     6661 agcaaatatt taaattgtaa acgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     6721 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     6781 atagcccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     6841 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     6901 accatcaccc aaatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     6961 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     7021 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     7081 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtaaa aggatctagg
     7141 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     7201 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     7261 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     7321 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     7381 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     7441 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     7501 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     7561 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     7621 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     7681 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     7741 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     7801 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     7861 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     7921 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     7981 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc
     8041 tgtgcggtat ttcacaccgc atgtggtgca ctctcagtac accgcatgtg gtgcactctc
     8101 agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg
     8161 actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     8221 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     8281 agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt
     8341 ggtcgtgcag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct
     8401 ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct
     8461 gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga
     8521 tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg
     8581 aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc
     8641 agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc
     8701 atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac
     8761 tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc
     8821 agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc
     8881 aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc
     8941 aggacccaac gctgcccgaa attccgacac catcgaatgg tgcaaaacct ttcgcggtat
     9001 ggcatgatag cgcccggaag agagtcaatt cagggtggtg aatgtgaaac cagtaacgtt
     9061 atacgatgtc gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca
     9121 ggccagccac gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa
     9181 ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt
     9241 tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg
     9301 cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc
     9361 ctgtaaagcg gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga tcattaacta
     9421 tccgctggat gaccaggatg ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt
     9481 atttcttgat gtctctgacc agacacccat caacagtatt attttctccc atgaagacgg
     9541 tacgcgactg ggcgtggagc atctggtcgc attgggtcac cagcaaatcg cgctgttagc
     9601 gggcccatta agttctgtct cggcgcgtct gcgtctggct ggctggcata aatatctcac
     9661 tcgcaatcaa attcagccga tagcggaacg ggaaggcgac tggagtgcca tgtccggttt
     9721 tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc actgcgatgc tggttgccaa
     9781 cgatcagatg gcgctgggcg caatgcgcgc cattaccgag tccgggctgc gcgttggtgc
     9841 ggatatctcg gtagtgggat acgacgatac cgaagacagc tcatgttata tcccgccgtt
     9901 aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca
     9961 actctctcag ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag
    10021 aaaaaccacc ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
    10081 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
    10141 atgtaagtta gctcactcat taggcacaat tctcatgttt gacagcttat catcgactgc
    10201 acggtgcacc aatgcttctg gcgtcaggca gccatcggaa gctgtggtat ggctgtgcag
    10261 gtcgtaaatc actgcataat tcgtgtcgct caaggcgcac tcccgttctg gataatgttt
    10321 tttgcgccga catcataacg gttctggcaa atattctgaa atgagctg
//
