LOCUS       sequence-263318-        8274 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression plasmid for (SUMO)5 client to assess SUMO-SIM 
            scaffolds..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8274)
  AUTHORS   Banani SF, Rice AM, Peeples WB, Lin Y, Jain S, Parker R, Rosen MK
  TITLE     Compositional Control of Phase-Separated Cellular Bodies.
  JOURNAL   Cell. 2016 Jul 28;166(3):651-663. doi: 10.1016/j.cell.2016.06.010. 
            Epub 2016 Jun 30.
  PUBMED    27374333
REFERENCE   2  (bases 1 to 8274)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.06.010"; journalName: "Cell"; date: 
            "2016-07-28- 28"; volume: "166"; issue: "3"; pages: "651-663"
COMMENT     Sequence Label: (SUMO)5
FEATURES             Location/Qualifiers
     source          1..8274
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      116..202
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      294..321
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        340..431
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(432..500,501..1292)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(650..669)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(1334..1847)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1529..1550)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1741..1760
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      1958..2546
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2447..2466
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2700..2717
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2823..2842)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2983..3005
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2999..3190)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        3686..3763
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             3764..4846
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(3783..3802)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        join(5089..5094,5095..5110,5111..5117)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    5125..5141
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5130..5152
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             5211..6311
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKTEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     regulatory      6168..6177
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     6285..6308
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             6360..6371
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             6384..6404
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             7974..7994
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             7995..8012
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(8025..8042)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8025..8041)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8034..8056)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8220..8237)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 ggcggcagta gcgcggtggt cccacctgac cccatgccga actcagaagt gaaacgccgt
       61 agcgccgatg gtagtgtggg gtctccccat gcgagagtag ggaactgcca ggcatcaaat
      121 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
      181 cgctctcctg agtaggacaa atccgccggg agcggatttg aacgttgcga agcaacggcc
      241 cggagggtgg cgggcaggac gcccgccata aactgccagg catcaaatta agcagaaggc
      301 catcctgacg gatggccttt ttgcgtttct acaaactctt ttgtttattt ttctaaatac
      361 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      421 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      481 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      541 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      601 gttttcgccc cgaagaacgt ttcccaatga tgagcacttt taaagttctg ctatgtggcg
      661 cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      721 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      781 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      841 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      901 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      961 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     1021 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     1081 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     1141 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     1201 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     1261 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1321 tttagattga tttaccccgg ttgataatca gaaaagcccc aaaaacagga agattgtata
     1381 agcaaatatt taaattgtaa acgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     1441 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     1501 atagcccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     1561 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     1621 accatcaccc aaatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     1681 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     1741 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     1801 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtaaa aggatctagg
     1861 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     1921 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     1981 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     2041 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     2101 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     2161 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     2221 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2281 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2341 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     2401 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     2461 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     2521 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     2581 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     2641 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     2701 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc
     2761 tgtgcggtat ttcacaccgc atgtggtgca ctctcagtac accgcatgtg gtgcactctc
     2821 agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg
     2881 actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     2941 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     3001 agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt
     3061 ggtcgtgcag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct
     3121 ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct
     3181 gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga
     3241 tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg
     3301 aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc
     3361 agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc
     3421 atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac
     3481 tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc
     3541 agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc
     3601 aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc
     3661 aggacccaac gctgcccgaa attccgacac catcgaatgg tgcaaaacct ttcgcggtat
     3721 ggcatgatag cgcccggaag agagtcaatt cagggtggtg aatgtgaaac cagtaacgtt
     3781 atacgatgtc gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca
     3841 ggccagccac gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa
     3901 ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt
     3961 tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg
     4021 cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc
     4081 ctgtaaagcg gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga tcattaacta
     4141 tccgctggat gaccaggatg ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt
     4201 atttcttgat gtctctgacc agacacccat caacagtatt attttctccc atgaagacgg
     4261 tacgcgactg ggcgtggagc atctggtcgc attgggtcac cagcaaatcg cgctgttagc
     4321 gggcccatta agttctgtct cggcgcgtct gcgtctggct ggctggcata aatatctcac
     4381 tcgcaatcaa attcagccga tagcggaacg ggaaggcgac tggagtgcca tgtccggttt
     4441 tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc actgcgatgc tggttgccaa
     4501 cgatcagatg gcgctgggcg caatgcgcgc cattaccgag tccgggctgc gcgttggtgc
     4561 ggatatctcg gtagtgggat acgacgatac cgaagacagc tcatgttata tcccgccgtt
     4621 aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca
     4681 actctctcag ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag
     4741 aaaaaccacc ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     4801 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     4861 atgtaagtta gctcactcat taggcacaat tctcatgttt gacagcttat catcgactgc
     4921 acggtgcacc aatgcttctg gcgtcaggca gccatcggaa gctgtggtat ggctgtgcag
     4981 gtcgtaaatc actgcataat tcgtgtcgct caaggcgcac tcccgttctg gataatgttt
     5041 tttgcgccga catcataacg gttctggcaa atattctgaa atgagctgtt gacaattaat
     5101 catcggctcg tataatgtgt ggaattgtga gcggataaca atttcacaca ggaaacagcc
     5161 agtccgttta ggtgttttca cgagcacttc accaacaagg accatagatt atgaaaactg
     5221 aagaaggtaa actggtaatc tggattaacg gcgataaagg ctataacggt ctcgctgaag
     5281 tcggtaagaa attcgagaaa gataccggaa ttaaagtcac cgttgagcat ccggataaac
     5341 tggaagagaa attcccacag gttgcggcaa ctggcgatgg ccctgacatt atcttctggg
     5401 cacacgaccg ctttggtggc tacgctcaat ctggcctgtt ggctgaaatc accccggaca
     5461 aagcgttcca ggacaagctg tatccgttta cctgggatgc cgtacgttac aacggcaagc
     5521 tgattgctta cccgatcgct gttgaagcgt tatcgctgat ttataacaaa gatctgctgc
     5581 cgaacccgcc aaaaacctgg gaagagatcc cggcgctgga taaagaactg aaagcgaaag
     5641 gtaagagcgc gctgatgttc aacctgcaag aaccgtactt cacctggccg ctgattgctg
     5701 ctgacggggg ttatgcgttc aagtatgaaa acggcaagta cgacattaaa gacgtgggcg
     5761 tggataacgc tggcgcgaaa gcgggtctga ccttcctggt tgacctgatt aaaaacaaac
     5821 acatgaatgc agacaccgat tactccatcg cagaagctgc ctttaataaa ggcgaaacag
     5881 cgatgaccat caacggcccg tgggcatggt ccaacatcga caccagcaaa gtgaattatg
     5941 gtgtaacggt actgccgacc ttcaagggtc aaccatccaa accgttcgtt ggcgtgctga
     6001 gcgcaggtat taacgccgcc agtccgaaca aagagctggc aaaagagttc ctcgaaaact
     6061 atctgctgac tgatgaaggt ctggaagcgg ttaataaaga caaaccgctg ggtgccgtag
     6121 cgctgaagtc ttacgaggaa gagttggcga aagatccacg tattgccgcc accatggaaa
     6181 acgcccagaa aggtgaaatc atgccgaaca tcccgcagat gtccgctttc tggtatgccg
     6241 tgcgtactgc ggtgatcaac gccgccagcg gtcgtcagac tgtcgatgaa gccctgaaag
     6301 acgcgcagac taattcgagc tcgaacaaca acaacaataa caataacaac aacctcggga
     6361 tcgagggaag gatttcagaa tttgaaaacc tgtattttca gggccatatg ggtgggagtt
     6421 ggggagggtc tatgtcagaa gagaagccta aggagggcgt gaaaaccgag aatgaccaca
     6481 ttaacttgaa ggttgctgga caggatgggt ccgtggttca gttcaaaatt aaacgccaca
     6541 caccgttgtc gaaactgatg aaagcgtaca gcgaacgcca gggtctttct atgcgtcaga
     6601 ttcgcttccg ctttgatggt caaccgatta atgaaacgga cacgccggca cagctggaga
     6661 tggaggatga agacactatc gacgtatttc agcagcagac cgtcgtaggg ggctccggcg
     6721 gaagcggggg gtcgggaggt tccatgagcg aagagaaacc aaaagaagga gttaaaaccg
     6781 aaaacgacca tatcaacctg aaggtcgccg gtcaggacgg ctcagtcgtg cagttcaaga
     6841 ttaaacgtca taccccatta agtaagctga tgaaggcgta tagtgagcgc caaggcttgt
     6901 cgatgcgcca gattcgtttt cgctttgatg gtcagccaat caacgaaacc gacaccccgg
     6961 cgcagctgga aatggaagat gaagatacca tcgatgtgtt ccaacagcaa acggttgttg
     7021 gcggaagcgg cgggagcggt ggtagcgggg gttctatgtc cgaagaaaaa ccgaaagagg
     7081 gtgtgaagac tgaaaacgac cacattaatc tgaaggtggc gggccaggac ggttccgttg
     7141 ttcagtttaa aatcaaacgt cacaccccgc tgtcgaaact gatgaaagcc tatagcgaac
     7201 gtcaagggct gagcatgcgt caaattcgtt ttcgctttga tggtcagcca atcaacgaaa
     7261 ccgatacacc ggcgcagctg gagatggaag acgaggacac cattgacgtc ttccagcaac
     7321 aaactgtagt gggtggctcc ggtggctctg gtgggtctgg tggctcgatg agtgaggaaa
     7381 aacccaaaga aggcgtcaag actgagaacg atcatattaa cctgaaagtc gcgggtcaag
     7441 atggttcagt ggtgcagttc aaaattaaac gccacacccc gctgtcaaag ctgatgaaag
     7501 cctatagtga gcgtcaaggt ctgtcgatgc gccagatccg ctttcgcttt gatggtcagc
     7561 caatcaacga gacagacact ccggcacaac tggagatgga agatgaagac accatcgatg
     7621 tttttcaaca gcagaccgtg gtgggaggga gcggcggtag cgggggatca ggtggtagta
     7681 tgtctgagga aaagccgaag gaaggggtaa agaccgaaaa tgatcacatt aatctgaaag
     7741 ttgcgggcca agatggtagt gtagtgcaat ttaaaatcaa gcgtcacacc ccgttatcta
     7801 aactgatgaa agcatatagc gaacgccaag gcctgtctat gcgccagatt cgctttcgct
     7861 ttgacgggca accaattaat gaaacggaca ccccggcaca actggaaatg gaagacgagg
     7921 atactattga cgtgtttcag caacagacag tggtcggcgg ctccggtgga tccgaaaacc
     7981 tgtattttca gggccatcat catcatcatc actgaaagct tggcactggc cgtcgtttta
     8041 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     8101 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     8161 cgcagcctga atggcgaatg gcagcttggc tgttttggcg gatgagataa gattttcagc
     8221 ctgatacaga ttaaatcaga acgcagaagc ggtctgataa aacagaattt gcct
//
