LOCUS       sequence-263339-        8729 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FRET biosensor. To visualize Shp2 activity by FRET.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8729)
  AUTHORS   Sun J, Lei L, Tsai CM, Wang Y, Shi Y, Ouyang M, Lu S, Seong J, Kim 
            TJ, Wang P, Huang M, Xu X, Nizet V, Chien S, Wang Y
  TITLE     Engineered proteins with sensing and activating modules for 
            automated reprogramming of cellular functions.
  JOURNAL   Nat Commun. 2017 Sep 7;8(1):477. doi: 10.1038/s41467-017-00569-6.
  PUBMED    28883531
REFERENCE   2  (bases 1 to 8729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-09-7"; volume: "8(1):477. doi"; pages: " 
            10.1038/s41467-017-00569-6"
COMMENT     Sequence Label: Shp2 biosensor
FEATURES             Location/Qualifiers
     source          1..8729
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        130..509
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        510..713
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     664..684
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     758..777
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        758..776
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      796..805
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             817..834
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             838..870
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(874..882,883..897)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             join(904..906,907..909,910..1422)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIE"
     primer_bind     complement(949..970)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1210..1229)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1557..1578
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3400..4110
                     /codon_start=1
                     /product="yellow fluorescent protein optimized for FRET 
                     with CyPet (Nguyen and Daugherty, 2005)"
                     /label=YPet
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKL
                     LCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKAN
                     FKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLLEF
                     LTAAGITEGMNELYK"
     promoter        complement(4177..4195)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4178..4195)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4215..4232)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4221..4445
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4491..4919
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4578..4597)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4788..4809
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4928..4948)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4933..5262
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5113..5248
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5175..5194
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5329..6123
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5383..5402)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5993..6012
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6297..6418
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6334..6353)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6388..6407
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6467..6483)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6467..6483)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6480..6502)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6491..6507
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6515..6521,6522..6539,6540..6545))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6560..6581
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6698..6715)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6869..7457)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6949..6968)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7628..8419,8420..8488))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8251..8270
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8489..8593)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8668..8687)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt
       61 gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat
      121 atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
      181 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      241 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      301 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
      361 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      421 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      481 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      541 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      601 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      661 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc
      721 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga
      781 cccaagcttg cggccgccac catggtgcgg ggttctcatc atcatcatca tcatggtatg
      841 gctagcatga ctggtggaca gcaaatgggt cgggatctgt acgacgatga cgataaggat
      901 cccatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
      961 gacggcgacg taaacggcca caggttcagc gtgtccggcg agggcgaggg cgatgccacc
     1021 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     1081 accctcgtga ccaccctgac ctggggcgtg cagtgcttca gccgctaccc cgaccacatg
     1141 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgtaccatc
     1201 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     1261 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     1321 cacaagctgg agtacaacta catcagccac aacgtctata tcaccgccga caagcagaag
     1381 aacggcatca aggcccactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
     1441 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     1501 cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
     1561 gtcctgctgg agttcgtgac cgccgcccgc atgcatggag gcagcatgac atcgcggaga
     1621 tggtttcacc caaatatcac tggtgtggag gcagaaaacc tactgttgac aagaggagtt
     1681 gatggcagtt ttttggcaag gcctagtaaa agtaaccctg gagacttcac actttccgtt
     1741 agaagaaatg gagctgtcac ccacatcaag attcagaaca ctggtgatta ctatgacctg
     1801 tatggagggg agaaatttgc cactttggct gagttggtcc agtattacat ggaacatcac
     1861 gggcaattaa aagagaagaa tggagatgtc attgagctta aatatcctct gaactgtgca
     1921 gatcctacct ctgaaaggtg gtttcatgga catctctctg ggaaagaagc agagaaatta
     1981 ttaactgaaa aaggaaaaca tggtagtttt cttgtacgag agagccagag ccaccctgga
     2041 gattttgttc tttctgtgcg cactggtgat gacaaagggg agagcaatga cggcaagtct
     2101 aaagtgaccc atgttatgat tcgctgtcag gaactgaaat acgacgttgg tggaggagaa
     2161 cggtttgatt ctttgacaga tcttgtggaa cattataaga agaatcctat ggtggaaaca
     2221 ttgggtacag tactacaact caagcagccc cttaacacga ctcgtataaa tgctgctgaa
     2281 atagaaagca gagttcgaga actaagcaaa ttagctgaga ccacagataa agtcaaacaa
     2341 ggcttttggg aagaatttga gacactacaa caacaggagt gcaaacttct ctacagccga
     2401 aaagagggtc aaaggcaaga aaacaaaaac aaaaatagat ataaaaacat cctgcccttt
     2461 gatcatacca gggttgtcct acacgatggt gatcccaatg agcctgtttc agattacatc
     2521 aatgcaaata tcatcatgcc tgaatttgaa accaagtgca acaattcaaa gcccaaaaag
     2581 agttacattg ccacacaagg ctgcctgcaa aacacggtga atgacttttg gcggatggtg
     2641 ttccaagaaa actcccgagt gattgtcatg acaacgaaag aagtggagag aggaaagagt
     2701 aaatgtgtca aatactggcc tgatgagtat gctctaaaag aatatggcgt catgcgtgtt
     2761 aggaacgtca aagaaagcgc cgctcatgac tatacgctaa gagaacttaa actttcaaag
     2821 gttggacaag ggaatacgga gagaacggtc tggcaatacc actttcggac ctggccggac
     2881 cacggcgtgc ccagcgaccc tgggggcgtg ctggacttcc tggaggaggt gcaccataag
     2941 caggagagca tcatggatgc agggccggtc gtggtgcact gcagtgctgg aattggccgg
     3001 acagggacgt tcattgtgat tgatattctt attgacatca tcagagagaa aggtgttgac
     3061 tgcgatattg acgttcccaa aaccatccag atggtgcggt ctcagaggtc agggatggtc
     3121 cagacagaag cacagtaccg atttatctat atggcggtcc agcattatat tgaaacacta
     3181 cagcgcagga ttgaagaaga gcagaaaagc aagaggaaag ggcacgaata tacaaatatt
     3241 aagtattctc tagcggacca gactagtgga gatcagagcc ctctcccgcc ttgtactcca
     3301 acgccaccct gtgcagaaat gagagaagac agtgctagag tctatgaaaa cgtgggcctg
     3361 atgcaacagc agaaaagttt cagaggaggc agcgagctct ctaaaggtga agaattattc
     3421 actggtgttg tcccaatttt ggttgaatta gatggtgatg ttaatggtca caaattttct
     3481 gtctccggtg aaggtgaagg tgatgctacg tacggtaaat tgaccttaaa attactctgt
     3541 actactggta aattgccagt tccatggcca accttagtca ctactttagg ttatggtgtt
     3601 caatgttttg ctagataccc agatcatatg aaacaacatg actttttcaa gtctgccatg
     3661 ccagaaggtt atgttcaaga aagaactatt tttttcaaag atgacggtaa ctacaagacc
     3721 agagctgaag tcaagtttga aggtgatacc ttagttaata gaatcgaatt aaaaggtatt
     3781 gattttaaag aagatggtaa cattttaggt cacaaattgg aatacaacta taactctcac
     3841 aatgtttaca tcactgctga caaacaaaag aatggtatca aagctaactt caaaattaga
     3901 cacaacattg aagatggtgg tgttcaatta gctgaccatt atcaacaaaa tactccaatt
     3961 ggtgatggtc cagtcttgtt accagacaac cattacttat cctatcaatc tgccttattc
     4021 aaagatccaa acgaaaagag agaccacatg gtcttgttag aatttttgac tgctgctggt
     4081 attaccgagg gtatgaatga attgtacaaa tagtaagaat tctgcagata tccatcacac
     4141 tggcggccgc tcgagcatgc atctagaggg ccctattcta tagtgtcacc taaatgctag
     4201 agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     4261 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     4321 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     4381 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     4441 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg
     4501 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     4561 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     4621 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     4681 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     4741 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     4801 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     4861 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta
     4921 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     4981 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     5041 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     5101 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     5161 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag
     5221 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt
     5281 atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa
     5341 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
     5401 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
     5461 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca
     5521 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
     5581 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
     5641 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
     5701 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
     5761 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
     5821 ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc
     5881 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     5941 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     6001 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     6061 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     6121 tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag
     6181 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     6241 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
     6301 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     6361 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     6421 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     6481 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     6541 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     6601 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     6661 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     6721 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     6781 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     6841 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     6901 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     6961 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     7021 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     7081 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     7141 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     7201 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     7261 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     7321 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     7381 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     7441 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     7501 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     7561 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     7621 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     7681 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     7741 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     7801 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     7861 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     7921 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     7981 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     8041 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     8101 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     8161 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     8221 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     8281 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     8341 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     8401 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     8461 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     8521 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     8581 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
     8641 tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     8701 gtatctgctc cctgcttgtg tgttggagg
//
