LOCUS       sequence-263341-        3421 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SapTrap mNG-C1 donor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3421)
  TITLE     Unpublished SapTrap/SEC plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3421)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pDD346
FEATURES             Location/Qualifiers
     source          1..3421
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      397..406
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(960..1059)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1076..1095)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1077..1095)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1100..1116)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1100..1116)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1218..1237)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1229..2038
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1296..1315)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2185..2773
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2674..2693
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2927..2944
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3103..3130)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3222..3308)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3357..3379
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3371..3388
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3372..3388
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    join(3404..3421,1..82)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 tgactgatag tgacctgttc gttgcaacaa attgatgagc aatgcttttt tataatgcca
       61 actttgtaca aaaaagcagg ctgctcttcg atggtctcca agggagagga ggacaacatg
      121 gcctccctcc cagccaccca cgagctccac atcttcggat ccatcaacgg agtcgacttc
      181 gacatggtcg gacaaggaac cggaaaccca aacgacggat acgaggagct caacctcaag
      241 tccaccaagg taagtttaaa catatatata ctaactaacc ctgattattt aaattttcag
      301 ggagacctcc aattctcccc atggatcctc gtcccacaca tcggatacgg attccaccaa
      361 tacctcccat acccagacgg aatgtcccca ttccaagccg ccatggtcga cggatccgga
      421 taccaagtcc accgtaccat gcaattcgag gacggagcct ccctcaccgt caactaccgt
      481 tacacctacg agggatccca catcaaggta agtttaaaca gttcggtact aactaaccat
      541 acatatttaa attttcaggg agaggcccaa gtcaagggaa ccggattccc agccgacgga
      601 ccagtcatga ccaactccct caccgccgcc gactggtgcc gttccaagaa gacctaccca
      661 aacgacaagg taagtttaaa catgatttta ctaactaact aatctgattt aaattttcag
      721 accatcatct ccaccttcaa gtggtcctac accaccggaa acggaaagcg ttaccgttcc
      781 accgcccgta ccacctacac cttcgccaag ccaatggccg ccaactacct caagaaccaa
      841 ccaatgtacg tcttccgtaa gaccgagctc aagcactcca agaccgagct caacttcaag
      901 gagtggcaaa aggccttcac cgacgtcatg ggaatggacg agctctacaa gcgaagagca
      961 cccagctttc ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa
     1021 cgaacaggtc actatcagtc aaaataaaat cattatttgc catccagctg atatccccta
     1081 tagtgagtcg tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa
     1141 tctctgatgt tacattgcac aagataaaaa tatatcatca tgaacaataa aactgtctgc
     1201 ttacataaac agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcgaggcc
     1261 gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt
     1321 cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc cagagttgtt
     1381 tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa
     1441 ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga
     1501 tgcatggtta ctcaccactg cgatccccgg aaaaacagca ttccaggtat tagaagaata
     1561 tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc
     1621 gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca
     1681 atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg
     1741 gcctgttgaa caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt
     1801 cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg
     1861 ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg
     1921 gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat
     1981 tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc
     2041 agaattggtt aattggttgt aacactggca gagcattacg ctgacttgac gggacggcgc
     2101 aagctcatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc
     2161 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     2221 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     2281 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     2341 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     2401 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     2461 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     2521 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     2581 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     2641 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     2701 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     2761 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     2821 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     2881 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     2941 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     3001 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     3061 taatacgcgt accgctagcc aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg
     3121 atggccttct gcttagtttg atgcctggca gtttatggcg ggcgtcctgc ccgccaccct
     3181 ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg gatttgtcct actcaggaga
     3241 gcgttcaccg acaaacaaca gataaaacga aaggcccagt cttccgactg agcctttcgt
     3301 tttatttgat gcctggcagt tccctactct cgcgttaacg ctagcatgga tgttttccca
     3361 gtcacgacgt tgtaaaacga cggccagtct taagctcggg ccccaaataa tgattttatt
     3421 t
//
