LOCUS       sequence-263355-       10743 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A lentiviral vector that constitutively expresses an error-prone 
            variant of the adenovirus DNA polymerase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10743)
  AUTHORS   Berman CM, Papa LJ 3rd, Hendel SJ, Moore CL, Suen PH, Weickhardt AF,
            Doan ND, Kumar CM, Uil TG, Butty VL, Hoeben RC, Shoulders MD
  TITLE     An Adaptable Platform for Directed Evolution in Human Cells.
  JOURNAL   J Am Chem Soc. 2018 Dec 26;140(51):18093-18103. doi: 
            10.1021/jacs.8b10937. Epub 2018 Dec 14.
  PUBMED    30427676
REFERENCE   2  (bases 1 to 10743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.8b10937"; journalName: "J Am Chem Soc"; date: 
            "2018-12-26- 26"; volume: "140"; issue: "51"; pages: "18093-18103"
COMMENT     Sequence Label: EP-Pol vector
FEATURES             Location/Qualifiers
     source          1..10743
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(1651..1688,1689..1754)
                     /label=Ad SA
                     /note="adenovirus major late transcript splice acceptor 
                     (Friedrich and Soriano, 1991)"
     CDS             3600..3626
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     LTR             4719..4952
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5024..5145
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5061..5080)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5115..5134
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5185..5320
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5340..5359)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5341..5359)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5369..5386)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5369..5385)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5378..5400)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5527..5982
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5614..5633)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5824..5845
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6008..6112
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6113..6181,6182..6973)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6331..6350)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7144..7732
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7633..7652
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7886..7903
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8020..8041
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8056..8061,8062..8079,8080..8086)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8094..8110
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8099..8121
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8118..8134
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8118..8134
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8153..8173
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8155..8173
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8201..8427
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8428..8608
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8655..8780
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9273..9506
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9691..9735
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9997..10114
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        10137..10440
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10441..10644
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10595..10615
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     10641..10665
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 aattcatggc cttggttcaa gctcaccggg cccgtcgtct tcacgcagag gcgccagatt
       61 caggagatca accgccgcgt cgtcgcgttc gccagcaacc tacgcgcgca gcaccagctc
      121 ctgcccgcgc gcggcgccga cgtgcccctg ccccctctcc cggcgggtcc ggagcccccc
      181 ctacctccgg gggctcgccc gcgtcaccgc ttttagatgc atcatccaag gacacccccg
      241 cggcccaccg cccgccgcgc ggtaccgtag tcgcgccgcg gggatgcggc ctcttgcaag
      301 ccatcgacgc cgccaccaac cagcccctgg aaattaggta tcacctggat ctagcccgcg
      361 ccctgacccg tctatgcgag gtaaacctgc aggagctccc gcctgacctg acgccgcggg
      421 agctccagac catggacagc tcccatctgc gcgatgttgt catcaagctc cgaccgccgc
      481 gcgcggacat ctggactttg ggctcgcgcg gcgtggtggt ccgatccacc gtaactcccc
      541 tcgagcagcc agacggtcaa ggacaagcag ccgaagtaga agaccaccag ccaaacccgc
      601 caggcgaggg gctcaaattc ccactctgct tccttgtgcg cggtcgtcag gtcaacctcg
      661 tgcaggatgt acagcccgtg caccgctgcc agtactgcgc acgtttttac aaaagccagc
      721 acgagtgttc ggcccgtcgc agggacttct actttcacca catcaatagc cactcctcca
      781 attggtggcg ggagatccag ttcttcccga tcggctcgca tcctcgcacc gagcgtctct
      841 ttgtcaccta cgatgtagag acctatactt ggatgggggc ctttgggaag cagctcgtgc
      901 ccttcatgct ggtcatgaag ttcggcggag atgagcctct agtgactgcc gcgcgagacc
      961 tagccgcgaa ccttggatgg gaccgctggg aacaagaccc gcttaccttc tactgcatca
     1021 ccccagaaaa aatggccata ggtcgccagt ttaggacctt tcgcgaccac ctgcaaatgc
     1081 taatggcccg tgacctgtgg agctcattcg tcgcttccaa ccctcatctt gcagactggg
     1141 ccctttcaga gcacgggctc agctcccctg aagagctcac ctacgaggaa cttaaaaaat
     1201 tgccttccat caagggcatc ccgcgcttct tggaacttta cattgtgggc cacaacatta
     1261 atgggtacga cgagatcgtg ctcgccgccc aggtaattaa caaccgttcc gaggtgccgg
     1321 gacccttccg catcacacgc aactttatgc ctcgcgcggg aaagatactc ttcaacgatg
     1381 tcaccttcgc cctgccaaat ccgcgttcca aaaagcgcac ggactttttg ctctgggagc
     1441 agggcggatg cgacgacact gacttcaaat accagtacct caaagtcatg gtcagggaca
     1501 cctttgcgct cacccacacc tcgctccgga aggccgcgca ggcatacgcg ctacccgtag
     1561 aaaagggatg ctgcgcctac caggccgtca accagttcta catgctaggc tcttaccgtt
     1621 cggaggccga cgggtttccg atccaagagt actggaaaga ccgcgaagag tttgtcctca
     1681 accgcgagct gtggaaaaaa aagggacagg ataagtatga catcatcaag gaaaccctgg
     1741 actactgcgc cctagacgtg caggtcaccg ccgagctggt caacaagctg cgcgactcct
     1801 acgcctcctt cgtgcgtgac gcggtaggtc tcacagacgc cagcttcaac gtcttccagc
     1861 gtccaaccat atcatccaac tcacatgcca tcttcaggca gatagtcttc cgagcagagc
     1921 agcccgcccg tagcaacctc ggtcccgacc tcctcgctcc ctcgcacgaa ctatacgatt
     1981 acgtgcgcgc cagcatccgc ggtggaagat gctaccctac atatcttgga atactcagag
     2041 agcccctcta cgtttacgac atttgcggca tgtacgcctc cgcgctcacc caccccatgc
     2101 catggggtcc cccactcaac ccatacgagc gcgcgcttgc cgcccgcgca tggcagcagg
     2161 cgctagactt gcaaggatgc aagatagact acttcgacgc gcgcctgctg cccggggtct
     2221 ttaccgtgga cgcagacccc ccggacgaga cgcagctaga ccccctaccg ccattctgct
     2281 cgcgcaaggg cggccgcctc tgctggacca acgagcgcct acgcggagag gtagccacca
     2341 gcgttgacct tgtcaccctg cacaaccgcg gttggcgcgt gcacctggtg cccgacgagc
     2401 gcaccaccgt ctttcccgaa tggcggtgcg ttgcgcgcga atacgtgcag ctaaacatcg
     2461 cggccaagga gcgcgccgat cgcgctaaga accaaaccct gcgctccatc gccaagttgc
     2521 tgtccaacgc cctctacggg tcgtttgcca ccaagcttga caacaaaaag attgtctttt
     2581 ctgaccagat ggatgcggcc accctcaaag gcatcaccgc gggccaggtg aatatcaaat
     2641 cctcctcgtt tttggaaact gacaatctta gcgcagaagt catgcccgct tttcagaggg
     2701 agtactcacc ccaacagctg gccctcgcag acagcgatgc ggaagagagt gaggacgaac
     2761 gcgcccccac ccccttttat agcccccctt caggaacacc cggtcacgtg gcctacacct
     2821 acaaaccaat caccttcctt gatgccgaag agggcgacat gtgtcttcac accctggagc
     2881 gagtggaccc cctagtggac aacgaccgct acccctccca cttagcctcc ttcgtgctgg
     2941 cctggacgcg agcctttgtc tcagagtggt ccgagtttct atacgaggag gaccgcggaa
     3001 caccgctcga ggacaggcct ctcaagtctg tatacgggga cacggacagc cttttcgtca
     3061 ccgagcgtgg acaccggctc atggaaacca gaggtaagaa acgcatcaaa aagcatgggg
     3121 gaaacctggt ttttgacccc gaacggccag agctcacctg gctcgtggaa tgcgagaccg
     3181 tctgcggggc ctgcggcgcg gatgcctact ccccggaatc ggtatttctc gcgcccaagc
     3241 tctacgccct caaaagtctg cactgcccct cgtgcggcgc ctcctccaag ggcaagctgc
     3301 gcgccaaggg ccacgccgcg gaggggctgg actatgacac catggtcaaa tgctacctgg
     3361 ccgacgcgca gggcgaagac cggcagcgct tcagcaccag caggaccagc ctcaagcgca
     3421 ccctggccag cgcgcagccc ggagcgcacc ccttcaccgt gacccagact acgctgacga
     3481 ggaccctgcg cccgtggaaa gacatgaccc tggcccgtct ggacgagcac cgactactgc
     3541 cgtacagcga aagccgcccc aacccgcgaa acgaggagat atgctggatc gagatgccgt
     3601 acccatacga tgttccggat tacgcttaga gcacgtgact acatttaaac cctaacaaaa
     3661 caaagagatg gggttactct ctaaatttta tgggttatgt cattggatgt tatgggtcct
     3721 tgccacaaga acacatcata caaaaaatca aagaatgttt tagaaaactt cctattaaca
     3781 ggcctattga ttggaaagta tgtcaacgaa ttgtgggtct tttgggtttt gctgcccctt
     3841 ttacacaatg tggttatcct gcgttgatgc ctttgtatgc atgtattcaa tctaagcagg
     3901 ctttcacttt ctcgccaact tacaaggcct ttctgtgtaa acaatacctg aacctttacc
     3961 ccgttgcccg gcaacggcca ggtctgtgcc aagtgtttgc tgacgcaacc cccactggct
     4021 ggggcttggt catgggccat cagcgcatgc gtggaacctt ttcggctcct ctgccgatcc
     4081 atactgcgga actcctagcc gcttgttttg ctcgcagcag gtctggagca aacattatcg
     4141 ggactgataa ctctgttgtc ctatcccgca aatatacatc gtttccatgg ctgctaggct
     4201 gtgctgccaa ctggatcctg cgcgggacgt cctttgttta cgtcccgtcg gcgctgaatc
     4261 ctgcggacga cccttctcgg ggtcgcttgg gactctctcg tccccttctc cgtctgccgt
     4321 tccgaccgac cacggggcgc acctctcttt acgcggactc cccgtctgtg ccttctcatc
     4381 tgccggaccg tgtgcacttc gcttcacctc tgcacgtcgc atggagacca ccgtgaacgc
     4441 ccaccaaata ttgcccaagg tcttacataa gaggactctt ggactctcag caatgtcaac
     4501 gaccgacctt gaggcatact tcaaagactg tttgtttaaa gactgggagg agttggggga
     4561 ggagattagg ttaaaggtct ttgtactagg aggctgtagg cataaattgg tctgcgcacc
     4621 agcaccatgg cgcaatcact agagcggggt acctttaaga ccaatgactt acaaggcagc
     4681 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     4741 acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     4801 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     4861 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     4921 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca
     4981 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc
     5041 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     5101 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta
     5161 gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     5221 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     5281 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc
     5341 cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg
     5401 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     5461 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     5521 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     5581 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     5641 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     5701 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     5761 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     5821 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     5881 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     5941 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg
     6001 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     6061 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     6121 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     6181 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     6241 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     6301 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     6361 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     6421 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     6481 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     6541 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     6601 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     6661 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     6721 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     6781 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     6841 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     6901 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     6961 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     7021 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     7081 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     7141 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     7201 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     7261 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     7321 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     7381 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     7441 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     7501 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     7561 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     7621 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     7681 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     7741 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     7801 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     7861 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     7921 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     7981 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     8041 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     8101 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa
     8161 ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc
     8221 ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag
     8281 caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa
     8341 cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta
     8401 tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg
     8461 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     8521 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     8581 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
     8641 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
     8701 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
     8761 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
     8821 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
     8881 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
     8941 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
     9001 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
     9061 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
     9121 ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat
     9181 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga
     9241 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga
     9301 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta
     9361 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat
     9421 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa
     9481 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc
     9541 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat
     9601 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc
     9661 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
     9721 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
     9781 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
     9841 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
     9901 aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga
     9961 tccattcgat tagtgaacgg atctcgacgg tatcggtttt aaaagaaaag gggggattgg
    10021 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
    10081 attacaaaaa caaattacaa aaattcaaaa ttttatcgat aagcttggga gttccgcgtt
    10141 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
    10201 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
    10261 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
    10321 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
    10381 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
    10441 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
    10501 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
    10561 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
    10621 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
    10681 ccacgctgtt ttgacctcca tagaagacac cgactctagc tagaggatcc cccgggctgc
    10741 agg
//
