LOCUS       sequence-263358-        4065 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  adenoviral shuttle vector containing N-terminal Flag tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4065)
  AUTHORS   Lingfang Zeng, Qingbo Xu
  TITLE     pShuttle-Flag-HA vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pShuttle-Flag-HA
FEATURES             Location/Qualifiers
     source          1..4065
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(897..916)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     polyA_signal    926..1007
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     951..970
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(1029..1055)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(1056..1082)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(1155..1178)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(1200..1219)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1201..1219)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1264..1467)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(1293..1313)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        1468..1847
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     2019..2038
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2543..2562)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2554..3369
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             2554..3369
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2627..2646)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagaccca taatacccat
      841 aatgccattt cattacctct ttctccgcac ccgacataga tgaattcaga tctgcagatg
      901 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
      961 atgctattgc tttatttgta accattataa gctgcaataa acaagttcta gtcacttact
     1021 tacttaagcg cgtagtctgg cacgtcgtag gggtatgcat aatcaggaac atcatacgga
     1081 taatccaggg atcctctaga gtcgacctgc aggcatgcaa gcttatcgat acgcgtggta
     1141 ccagatctga attccttgtc atcgtcgtcc ttgtagtcca tgctagccag cttgggtctc
     1201 cctatagtga gtcgtattaa tttcgataag ccagtaagca gtgggttctc tagttagcca
     1261 gagagctctg cttatataga cctcccaccg tacacgccta ccgcccattt gcgtcaatgg
     1321 ggcggagttg ttacgacatt ttggaaagtc ccgttgattt tggtgccaaa acaaactccc
     1381 attgacgtca atggggtgga gacttggaaa tccccgtgag tcaaaccgct atccacgccc
     1441 attgatgtac tgccaaaacc gcatcaccat ggtaatagcg atgactaata cgtagatgta
     1501 ctgccaagta ggaaagtccc ataaggtcat gtactgggca taatgccagg cgggccattt
     1561 accgtcattg acgtcaatag ggggcgtact tggcatatga tacacttgat gtactgccaa
     1621 gtgggcagtt taccgtaaat actccaccca ttgacgtcaa tggaaagtcc ctattggcgt
     1681 tactatggga acatacgtca ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc
     1741 aggcgggcca tttaccgtaa gttatgtaac gcggaactcc atatatgggc tatgaactaa
     1801 tgaccccgta attgattact attaataact agtcaataat caatgtcaac gcgtatatct
     1861 ggcccgtaca tcgcgaagca gcgcaaaacg cctaacccta agcagattct tcatgcaatt
     1921 gtcggtcaag ccttgccttg ttgtagctta aattttgctc gcgcactact cagcgacctc
     1981 caacacacaa gcagggagca gatactggct taactatgcg gcatcagagc agattgtact
     2041 gagagtgcac cataggggat cgggagatct gagctttcgc taccttagga ccgttatagt
     2101 tacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     2161 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataatc
     2221 tagcgctgag gtctgcctcg tgaagaaggt gttgctgact cataccaggc ctgaatcgcc
     2281 ccatcatcca gccagaaagt gagggagcca cggttgatga gagctttgtt gtaggtggac
     2341 cagttggtga ttttgaactt ttgctttgcc acggaacggt ctgcgttgtc gggaagatgc
     2401 gtgatctgat ccttcaactc agcaaaagtt cgatttattc aacaaagcca cgttgtgtct
     2461 caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg
     2521 tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtct
     2581 tgctcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct
     2641 cgcgataatg tcgggcaatc aggtgcgaca atctatcgat tgtatgggaa gcccgatgcg
     2701 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg
     2761 gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt
     2821 actcctgatg atgcatggtt actcaccact gcgatccccg ggaaaacagc attccaggta
     2881 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc
     2941 cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc
     3001 gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag
     3061 cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataagctttt gccattctca
     3121 ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg
     3181 aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt
     3241 gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa
     3301 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag
     3361 tttttctaat cagaattggt taattggttg taacactggc agagcattac gctgacttga
     3421 cgggacggcg gctttgttga ataaatcgaa cttttgctga gttgaaggat cagatcacgc
     3481 atcttcccga caacgcagac cgttccgtgg caaagcaaaa gttcaaaatc accaactggt
     3541 ccacctacaa caaagctctc atcaaccgtg gctccctcac tttctggctg gatgatgggg
     3601 cgattcaggc ctggtatgag tcagcaacac cttcttcacg aggcagacct cagcgctcaa
     3661 agatgcaggg gtaaaagcta accgcatctt taccgacaag gcatccggca gttcaacaga
     3721 tcgggaaggg ctggatttgc tgaggatgaa ggtggaggaa ggtgatgtca ttctggtgaa
     3781 gaagctcgac cgtcttggcc gcgacaccgc cgacatgatc caactgataa aagagtttga
     3841 tgctcagggt gtagcggttc ggtttattga cgacgggatc agtaccgacg gtgatatggg
     3901 gcaaatggtg gtcaccatcc tgtcggctgt ggcacaggct gaacgccgga ggatcaaaag
     3961 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     4021 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttc
//
