LOCUS       sequence-263367-        9145 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transposon based vector that expresses human MYC followed by IRES 
            and firefly luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9145)
  AUTHORS   Ruiz de Galarreta M, Bresnahan E, Molina-Sanchez P, Lindblad KE, 
            Maier B, Sia D, Puigvehi M, Miguela V, Casanova-Acebes M, Dhainaut 
            M, Villacorta-Martin C, Singhi AD, Moghe A, von Felden J, Tal 
            Grinspan L, Wang S, Kamphorst AO, Monga SP, Brown BD, Villanueva A, 
            Llovet JM, Merad M, Lujambio A
  TITLE     beta-catenin activation promotes immune escape and resistance to 
            anti-PD-1 therapy in hepatocellular carcinoma.
  JOURNAL   Cancer Discov. 2019 Jun 11. pii: 2159-8290.CD-19-0074. doi: 
            10.1158/2159-8290.CD-19-0074.
  PUBMED    31186238
REFERENCE   2  (bases 1 to 9145)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Discov. 2019 Jun 11. pii: 2159-8290.CD-19-0074. doi: 
            10.1158/2159-8290.CD-19-0074."
COMMENT     Sequence Label: pT3-EF1A-MYC-IRES-luc
FEATURES             Location/Qualifiers
     source          1..9145
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    34..67
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        208..1386
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          439..1377
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1334..1354
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     1403..1422
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1403..1421
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1500..1524
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1533..2849
                     /codon_start=1
                     /product="human c-Myc proto-oncogene"
                     /label=c-Myc
                     /translation="MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAP
                     SEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTE
                     LLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPN
                     PARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDS
                     LLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGS
                     PSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNR
                     KCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKA
                     TAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA"
     protein_bind    complement(2851..2875)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2928..2969
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2991..3555
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3005..3557
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3149..3166)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3376..3395
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3569..5221
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(3635..3653)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     primer_bind     complement(5274..5291)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5280..5504
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    5574..5607
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     5663..5683
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(6065..6082)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6065..6081)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6074..6096)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(6290..6309)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6409..6431
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6469..6487)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6555..6659
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6660..6728,6729..7520)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6878..6897)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7691..8279
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8180..8199
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8433..8450
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8567..8588
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8603..8608,8609..8626,8627..8633)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8641..8657
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8646..8668
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8665..8681
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8665..8681
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9107..9126)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ctagctcgag atcttaatta aactagtatc gatataactt cgtataatgt atgctatacg
       61 aagttatatg tacgggccag atatacgcgt tgacattgat tattgactag gcttttgcaa
      121 aaagctttgc aaagatggat aaagttttaa acagagagga atctttgcag ctaatggacc
      181 ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc agagcgcaca
      241 tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga
      301 aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag
      361 ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg
      421 tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg cctctttacg
      481 ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt gattcttgat
      541 cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct
      601 tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg
      661 tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga
      721 tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg
      781 cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc
      841 acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct
      901 caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg
      961 gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc
     1021 cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca
     1081 cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag
     1141 taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta
     1201 ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa
     1261 gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga
     1321 tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg
     1381 tcgtgaggaa ttagcttggt actaatacga ctcactatag ggagacccaa gctggctagg
     1441 taagcttggt accgagctcg gatccactag tccagtgtgg tggaattctg cagatatcaa
     1501 caagtttgta caaaaaagca ggcttcgcca ccatgcccct caacgttagc ttcaccaaca
     1561 ggaactatga cctcgactac gactcggtgc agccgtattt ctactgcgac gaggaggaga
     1621 acttctacca gcagcagcag cagagcgagc tgcagccccc ggcgcccagc gaggatatct
     1681 ggaagaaatt cgagctgctg cccaccccgc ccctgtcccc tagccgccgc tccgggctct
     1741 gctcgccctc ctacgttgcg gtcacaccct tctcccttcg gggagacaac gacggcggtg
     1801 gcgggagctt ctccacggcc gaccagctgg agatggtgac cgagctgctg ggaggagaca
     1861 tggtgaacca gagtttcatc tgcgacccgg acgacgagac cttcatcaaa aacatcatca
     1921 tccaggactg tatgtggagc ggcttctcgg ccgccgccaa gctcgtctca gagaagctgg
     1981 cctcctacca ggctgcgcgc aaagacagcg gcagcccgaa ccccgcccgc ggccacagcg
     2041 tctgctccac ctccagcttg tacctgcagg atctgagcgc cgccgcctca gagtgcatcg
     2101 acccctcggt ggtcttcccc taccctctca acgacagcag ctcgcccaag tcctgcgcct
     2161 cgcaagactc cagcgccttc tctccgtcct cggattctct gctctcctcg acggagtcct
     2221 ccccgcaggg cagccccgag cccctggtgc tccatgagga gacaccgccc accaccagca
     2281 gcgactctga ggaggaacaa gaagatgagg aagaaatcga tgttgtttct gtggaaaaga
     2341 ggcaggctcc tggcaaaagg tcagagtctg gatcaccttc tgctggaggc cacagcaaac
     2401 ctcctcacag cccactggtc ctcaagaggt gccacgtctc cacacatcag cacaactacg
     2461 cagcgcctcc ctccactcgg aaggactatc ctgctgccaa gagggtcaag ttggacagtg
     2521 tcagagtcct gagacagatc agcaacaacc gaaaatgcac cagccccagg tcctcggaca
     2581 ccgaggagaa tgtcaagagg cgaacacaca acgtcttgga gcgccagagg aggaacgagc
     2641 taaaacggag cttttttgcc ctgcgtgacc agatcccgga gttggaaaac aatgaaaagg
     2701 cccccaaggt agttatcctt aaaaaagcca cagcatacat cctgtccgtc caagcagagg
     2761 agcaaaagct catttctgaa gaggacttgt tgcggaaacg acgagaacag ttgaaacaca
     2821 aacttgaaca gctacggaac tcttgtgcgg acccagcttt cttgtacaaa gtggttgata
     2881 tccagcacag tggcggccgc tcgagtctag agggcccgcg gttcgaaggt aagcctatcc
     2941 ctaaccctct cctcggtctc gattctacgc gtaccggtta gtaatgagtt tccccccccc
     3001 cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta
     3061 ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc
     3121 ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat
     3181 gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc
     3241 ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt
     3301 gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt
     3361 gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag
     3421 aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt
     3481 tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga
     3541 aaaacacgat gataatatgg ccacaaccat ggaagacgcc aaaaacataa agaaaggccc
     3601 ggcgccattc tatccgctgg aagatggaac cgctggagag caactgcata aggctatgaa
     3661 gagatacgcc ctggttcctg gaacaattgc ttttacagat gcacatatcg aggtggacat
     3721 cacttacgct gagtacttcg aaatgtccgt tcggttggca gaagctatga aacgatatgg
     3781 gctgaataca aatcacagaa tcgtcgtatg cagtgaaaac tctcttcaat tctttatgcc
     3841 ggtgttgggc gcgttattta tcggagttgc agttgcgccc gcgaacgaca tttataatga
     3901 acgtgaattg ctcaacagta tgggcatttc gcagcctacc gtggtgttcg tttccaaaaa
     3961 ggggttgcaa aaaattttga acgtgcaaaa aaagctccca atcatccaaa aaattattat
     4021 catggattct aaaacggatt accagggatt tcagtcgatg tacacgttcg tcacatctca
     4081 tctacctccc ggttttaatg aatacgattt tgtgccagag tccttcgata gggacaagac
     4141 aattgcactg atcatgaact cctctggatc tactggtctg cctaaaggtg tcgctctgcc
     4201 tcatagaact gcctgcgtga gattctcgca tgccagagat cctatttttg gcaatcaaat
     4261 cattccggat actgcgattt taagtgttgt tccattccat cacggttttg gaatgtttac
     4321 tacactcgga tatttgatat gtggatttcg agtcgtctta atgtatagat ttgaagaaga
     4381 gctgtttctg aggagccttc aggattacaa gattcaaagt gcgctgctgg tgccaaccct
     4441 attctccttc ttcgccaaaa gcactctgat tgacaaatac gatttatcta atttacacga
     4501 aattgcttct ggtggcgctc ccctctctaa ggaagtcggg gaagcggttg ccaagaggtt
     4561 ccatctgcca ggtatcaggc aaggatatgg gctcactgag actacatcag ctattctgat
     4621 tacacccgag ggggatgata aaccgggcgc ggtcggtaaa gttgttccat tttttgaagc
     4681 gaaggttgtg gatctggata ccgggaaaac gctgggcgtt aatcaaagag gcgaactgtg
     4741 tgtgagaggt cctatgatta tgtccggtta tgtaaacaat ccggaagcga ccaacgcctt
     4801 gattgacaag gatggatggc tacattctgg agacatagct tactgggacg aagacgaaca
     4861 cttcttcatc gttgaccgcc tgaagtctct gattaagtac aaaggctatc aggtggctcc
     4921 cgctgaattg gaatccatct tgctccaaca ccccaacatc ttcgacgcag gtgtcgcagg
     4981 tcttcccgac gatgacgccg gtgaacttcc cgccgccgtt gttgttttgg agcacggaaa
     5041 gacgatgacg gaaaaagaga tcgtggatta cgtcgccagt caagtaacaa ccgcgaaaaa
     5101 gttgcgcgga ggagttgtgt ttgtggacga agtaccgaaa ggtcttaccg gaaaactcga
     5161 cgcaagaaaa atcagagaga tcctcataaa ggccaagaag ggcggaaaga tcgccgtgta
     5221 attctaggat ccgtcgacct gcagccaagc ttatcgataa acccgctgat cagcctcgac
     5281 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     5341 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     5401 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     5461 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
     5521 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taaataactt
     5581 cgtataatgt atgctatacg aagttatatc gtatgcatgc tttgcatact tctgcctgct
     5641 ggggagcctg gggactttcc acaccctaac tgacacacat tccacagaat tccttgaaat
     5701 acatccacag gtacacctcc aattgactca aatgatgtca attagtctat cagaagcttc
     5761 taaagccatg acatcatttt ctggaatttt ccaagctgtt taaaggcaca gtcaacttag
     5821 tgtatgtaaa cttctgaccc actggaattg tgatacagtg aattataagt gaaataatct
     5881 gtctgtaaac aattgttgga aaaatgactt gtgtcatgca caaagtagat gtcctaactg
     5941 acttgccaaa actattgttt gttaacaaga aatttgtgga gtagttgaaa aacgagtttt
     6001 aatgactcca acttaagtgt atgtaaactt ccgacttcaa ctgtagggta ccgagctcga
     6061 attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     6121 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     6181 atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc
     6241 tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct
     6301 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     6361 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     6421 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
     6481 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     6541 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     6601 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     6661 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     6721 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     6781 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     6841 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     6901 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     6961 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     7021 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     7081 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     7141 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     7201 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     7261 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     7321 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     7381 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     7441 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     7501 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     7561 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     7621 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     7681 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     7741 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     7801 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     7861 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     7921 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     7981 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     8041 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     8101 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     8161 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     8221 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     8281 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     8341 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     8401 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     8461 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     8521 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     8581 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     8641 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
     8701 catgcctgca ggtcgactct agacctatac agttgaagtc ggaagtttac atacacttaa
     8761 gttggagtca ttaaaactcg tttttcaact actccacaaa tttcttgtta acaaacaata
     8821 gttttggcaa gtcagttagg acatctactt tgtgcatgac acaagtcatt tttccaacaa
     8881 ttgtttacag acagattatt tcacttataa ttcactgtat cacaattcca gtgggtcaga
     8941 agtttacata cactaagttg actgtgcctt taaacagctt ggaaaattcc agaaaatgat
     9001 gtcatggctt tagaagcttc tgatagacta attgacatca tttgagtcaa ttggaggtgt
     9061 acctgtggat gtatttcaag gggatccaga catgataaga tacattgatg agtttggaca
     9121 aaccacaact agaatgcagc ggccg
//
