LOCUS       sequence-263368-        9598 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transposon based vector that expresses human MYC followed by IRES 
            and firefly luciferase and the antigens SIYRYYGL (SIY), SIINFEKL 
            (SIN, OVA257-264), and OVA323-339.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9598)
  AUTHORS   Ruiz de Galarreta M, Bresnahan E, Molina-Sanchez P, Lindblad KE, 
            Maier B, Sia D, Puigvehi M, Miguela V, Casanova-Acebes M, Dhainaut 
            M, Villacorta-Martin C, Singhi AD, Moghe A, von Felden J, Tal 
            Grinspan L, Wang S, Kamphorst AO, Monga SP, Brown BD, Villanueva A, 
            Llovet JM, Merad M, Lujambio A
  TITLE     beta-catenin activation promotes immune escape and resistance to 
            anti-PD-1 therapy in hepatocellular carcinoma.
  JOURNAL   Cancer Discov. 2019 Jun 11. pii: 2159-8290.CD-19-0074. doi: 
            10.1158/2159-8290.CD-19-0074.
  PUBMED    31186238
REFERENCE   2  (bases 1 to 9598)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Discov. 2019 Jun 11. pii: 2159-8290.CD-19-0074. doi: 
            10.1158/2159-8290.CD-19-0074."
COMMENT     Sequence Label: pT3-EF1A-MYC-IRES-lucOS
FEATURES             Location/Qualifiers
     source          1..9598
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    34..67
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        208..1386
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          439..1377
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1334..1354
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     1403..1422
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1403..1421
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1500..1524
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1533..2849
                     /codon_start=1
                     /product="human c-Myc proto-oncogene"
                     /label=c-Myc
                     /translation="MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAP
                     SEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTE
                     LLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPN
                     PARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDS
                     LLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGS
                     PSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNR
                     KCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKA
                     TAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA"
     protein_bind    complement(2851..2875)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2928..2969
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2991..3555
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3005..3557
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3149..3166)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3376..3395
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3569..5218
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(3635..3653)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     CDS             5231..5254
                     /codon_start=1
                     /product="antigenic CD8+ T cell epitope from chicken 
                     ovalbumin"
                     /label=OVA peptide
                     /translation="SIINFEKL"
     CDS             5672..5701
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(5727..5744)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5733..5957
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    6027..6060
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     6116..6136
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(6518..6535)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6518..6534)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6527..6549)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(6743..6762)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6862..6884
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6922..6940)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7008..7112
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7113..7181,7182..7973)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7331..7350)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8144..8732
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8633..8652
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8886..8903
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9020..9041
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9056..9061,9062..9079,9080..9086)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9094..9110
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9099..9121
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9118..9134
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9118..9134
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9560..9579)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ctagctcgag atcttaatta aactagtatc gatataactt cgtataatgt atgctatacg
       61 aagttatatg tacgggccag atatacgcgt tgacattgat tattgactag gcttttgcaa
      121 aaagctttgc aaagatggat aaagttttaa acagagagga atctttgcag ctaatggacc
      181 ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc agagcgcaca
      241 tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga
      301 aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag
      361 ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg
      421 tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg cctctttacg
      481 ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt gattcttgat
      541 cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct
      601 tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg
      661 tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga
      721 tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg
      781 cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc
      841 acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct
      901 caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg
      961 gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc
     1021 cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca
     1081 cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag
     1141 taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta
     1201 ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa
     1261 gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga
     1321 tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg
     1381 tcgtgaggaa ttagcttggt actaatacga ctcactatag ggagacccaa gctggctagg
     1441 taagcttggt accgagctcg gatccactag tccagtgtgg tggaattctg cagatatcaa
     1501 caagtttgta caaaaaagca ggcttcgcca ccatgcccct caacgttagc ttcaccaaca
     1561 ggaactatga cctcgactac gactcggtgc agccgtattt ctactgcgac gaggaggaga
     1621 acttctacca gcagcagcag cagagcgagc tgcagccccc ggcgcccagc gaggatatct
     1681 ggaagaaatt cgagctgctg cccaccccgc ccctgtcccc tagccgccgc tccgggctct
     1741 gctcgccctc ctacgttgcg gtcacaccct tctcccttcg gggagacaac gacggcggtg
     1801 gcgggagctt ctccacggcc gaccagctgg agatggtgac cgagctgctg ggaggagaca
     1861 tggtgaacca gagtttcatc tgcgacccgg acgacgagac cttcatcaaa aacatcatca
     1921 tccaggactg tatgtggagc ggcttctcgg ccgccgccaa gctcgtctca gagaagctgg
     1981 cctcctacca ggctgcgcgc aaagacagcg gcagcccgaa ccccgcccgc ggccacagcg
     2041 tctgctccac ctccagcttg tacctgcagg atctgagcgc cgccgcctca gagtgcatcg
     2101 acccctcggt ggtcttcccc taccctctca acgacagcag ctcgcccaag tcctgcgcct
     2161 cgcaagactc cagcgccttc tctccgtcct cggattctct gctctcctcg acggagtcct
     2221 ccccgcaggg cagccccgag cccctggtgc tccatgagga gacaccgccc accaccagca
     2281 gcgactctga ggaggaacaa gaagatgagg aagaaatcga tgttgtttct gtggaaaaga
     2341 ggcaggctcc tggcaaaagg tcagagtctg gatcaccttc tgctggaggc cacagcaaac
     2401 ctcctcacag cccactggtc ctcaagaggt gccacgtctc cacacatcag cacaactacg
     2461 cagcgcctcc ctccactcgg aaggactatc ctgctgccaa gagggtcaag ttggacagtg
     2521 tcagagtcct gagacagatc agcaacaacc gaaaatgcac cagccccagg tcctcggaca
     2581 ccgaggagaa tgtcaagagg cgaacacaca acgtcttgga gcgccagagg aggaacgagc
     2641 taaaacggag cttttttgcc ctgcgtgacc agatcccgga gttggaaaac aatgaaaagg
     2701 cccccaaggt agttatcctt aaaaaagcca cagcatacat cctgtccgtc caagcagagg
     2761 agcaaaagct catttctgaa gaggacttgt tgcggaaacg acgagaacag ttgaaacaca
     2821 aacttgaaca gctacggaac tcttgtgcgg acccagcttt cttgtacaaa gtggttgata
     2881 tccagcacag tggcggccgc tcgagtctag agggcccgcg gttcgaaggt aagcctatcc
     2941 ctaaccctct cctcggtctc gattctacgc gtaccggtta gtaatgagtt tccccccccc
     3001 cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta
     3061 ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc
     3121 ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat
     3181 gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc
     3241 ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt
     3301 gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt
     3361 gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag
     3421 aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt
     3481 tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga
     3541 aaaacacgat gataatatgg ccacaaccat ggaagacgcc aaaaacataa agaaaggccc
     3601 ggcgccattc tatccgctgg aagatggaac cgctggagag caactgcata aggctatgaa
     3661 gagatacgcc ctggttcctg gaacaattgc ttttacagat gcacatatcg aggtggacat
     3721 cacttacgct gagtacttcg aaatgtccgt tcggttggca gaagctatga aacgatatgg
     3781 gctgaataca aatcacagaa tcgtcgtatg cagtgaaaac tctcttcaat tctttatgcc
     3841 ggtgttgggc gcgttattta tcggagttgc agttgcgccc gcgaacgaca tttataatga
     3901 acgtgaattg ctcaacagta tgggcatttc gcagcctacc gtggtgttcg tttccaaaaa
     3961 ggggttgcaa aaaattttga acgtgcaaaa aaagctccca atcatccaaa aaattattat
     4021 catggattct aaaacggatt accagggatt tcagtcgatg tacacgttcg tcacatctca
     4081 tctacctccc ggttttaatg aatacgattt tgtgccagag tccttcgata gggacaagac
     4141 aattgcactg atcatgaact cctctggatc tactggtctg cctaaaggtg tcgctctgcc
     4201 tcatagaact gcctgcgtga gattctcgca tgccagagat cctatttttg gcaatcaaat
     4261 cattccggat actgcgattt taagtgttgt tccattccat cacggttttg gaatgtttac
     4321 tacactcgga tatttgatat gtggatttcg agtcgtctta atgtatagat ttgaagaaga
     4381 gctgtttctg aggagccttc aggattacaa gattcaaagt gcgctgctgg tgccaaccct
     4441 attctccttc ttcgccaaaa gcactctgat tgacaaatac gatttatcta atttacacga
     4501 aattgcttct ggtggcgctc ccctctctaa ggaagtcggg gaagcggttg ccaagaggtt
     4561 ccatctgcca ggtatcaggc aaggatatgg gctcactgag actacatcag ctattctgat
     4621 tacacccgag ggggatgata aaccgggcgc ggtcggtaaa gttgttccat tttttgaagc
     4681 gaaggttgtg gatctggata ccgggaaaac gctgggcgtt aatcaaagag gcgaactgtg
     4741 tgtgagaggt cctatgatta tgtccggtta tgtaaacaat ccggaagcga ccaacgcctt
     4801 gattgacaag gatggatggc tacattctgg agacatagct tactgggacg aagacgaaca
     4861 cttcttcatc gttgaccgcc tgaagtctct gattaagtac aaaggctatc aggtggctcc
     4921 cgctgaattg gaatccatct tgctccaaca ccccaacatc ttcgacgcag gtgtcgcagg
     4981 tcttcccgac gatgacgccg gtgaacttcc cgccgccgtt gttgttttgg agcacggaaa
     5041 gacgatgacg gaaaaagaga tcgtggatta cgtcgccagt caagtaacaa ccgcgaaaaa
     5101 gttgcgcgga ggagttgtgt ttgtggacga agtaccgaaa ggtcttaccg gaaaactcga
     5161 cgcaagaaaa atcagagaga tcctcataaa ggccaagaag ggcggaaaga tcgccgtgga
     5221 gcagctcgag agtataatca actttgaaaa actgactgaa tggaccagtt ctaatgttat
     5281 ggaagagagg aagatcaaag tgtacttacc tcgcatgaag atggaggaaa aatacaacct
     5341 cacatctgtc ttaatggcta tgggcattac tgacgtgttt agctcttcag ccaatctgtc
     5401 tggcatctcc tcagcagaga gcctgaagat atctcaagct gtccatgcag cacatgcaga
     5461 aatcaatgaa gcaggcagag aggtggtagg gtcagcagag gctggagtgg atgctgcaag
     5521 cgtctctgaa gaatttaggg ctgaccatcc attcctcttc tgtatcaaac acatcgcaac
     5581 caacgccgtt ctcttctttg gcagatgtgt tggatccgag cagctggagt ccatctacag
     5641 gtactacggc ctgctgctga aggaaagatc cgaacaaaag cttatttctg aagaggactt
     5701 gtaagcggcc gaaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     5761 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     5821 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     5881 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     5941 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc
     6001 ccacgcgccc tgtagcggcg cattaaataa cttcgtataa tgtatgctat acgaagttat
     6061 atcgtatgca tgctttgcat acttctgcct gctggggagc ctggggactt tccacaccct
     6121 aactgacaca cattccacag aattccttga aatacatcca caggtacacc tccaattgac
     6181 tcaaatgatg tcaattagtc tatcagaagc ttctaaagcc atgacatcat tttctggaat
     6241 tttccaagct gtttaaaggc acagtcaact tagtgtatgt aaacttctga cccactggaa
     6301 ttgtgataca gtgaattata agtgaaataa tctgtctgta aacaattgtt ggaaaaatga
     6361 cttgtgtcat gcacaaagta gatgtcctaa ctgacttgcc aaaactattg tttgttaaca
     6421 agaaatttgt ggagtagttg aaaaacgagt tttaatgact ccaacttaag tgtatgtaaa
     6481 cttccgactt caactgtagg gtaccgagct cgaattcact ggccgtcgtt ttacaacgtc
     6541 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     6601 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     6661 tgaatggcga atggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     6721 accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
     6781 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     6841 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     6901 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     6961 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     7021 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     7081 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     7141 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     7201 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     7261 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     7321 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     7381 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     7441 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     7501 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     7561 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     7621 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     7681 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     7741 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     7801 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     7861 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     7921 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     7981 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     8041 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     8101 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     8161 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     8221 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     8281 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     8341 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     8401 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     8461 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     8521 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     8581 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     8641 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     8701 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     8761 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     8821 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     8881 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     8941 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     9001 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     9061 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     9121 gaaacagcta tgaccatgat tacgccaagc ttgcatgcct gcaggtcgac tctagaccta
     9181 tacagttgaa gtcggaagtt tacatacact taagttggag tcattaaaac tcgtttttca
     9241 actactccac aaatttcttg ttaacaaaca atagttttgg caagtcagtt aggacatcta
     9301 ctttgtgcat gacacaagtc atttttccaa caattgttta cagacagatt atttcactta
     9361 taattcactg tatcacaatt ccagtgggtc agaagtttac atacactaag ttgactgtgc
     9421 ctttaaacag cttggaaaat tccagaaaat gatgtcatgg ctttagaagc ttctgataga
     9481 ctaattgaca tcatttgagt caattggagg tgtacctgtg gatgtatttc aaggggatcc
     9541 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agcggccg
//
