LOCUS       sequence-263382-        1716 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used as a donor vector containing N-term 3xFLAG to clone into pSLIK.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Pereira EJ, Burns JS, Lee CY, Marohl T, Calderon D, Wang L, Atkins 
            KA, Wang CC, Janes KA
  TITLE     Sporadic activation of an oxidative stress-dependent NRF2-p53 
            signaling network in breast epithelial spheroids and 
            premalignancies.
  JOURNAL   Sci Signal. 2020 Apr 14;13(627). pii: 13/627/eaba4200. doi: 
            10.1126/scisignal.aba4200.
  PUBMED    32291314
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2020-04-14"; volume: "13(627). pii: 
            13/627/eaba4200. doi"; pages: " 10.1126/scisignal.aba4200"
COMMENT     Sequence Label: pEN_TTmiRC2_3xflag_Luciferase
FEATURES             Location/Qualifiers
     source          1..1716
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..69
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(136..154)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 atggactaca aagaccatga cggtgattat aaagatcatg acatcgatta caaggatgac
       61 gatgacaaga tggaagacgc caaaaacata aagaaaggcc cggcgccatt ctatccgctg
      121 gaagatggaa ccgctggaga gcaactgcat aaggctatga agagatacgc cctggttcct
      181 ggaacaattg cttttacaga tgcacatatc gaggtggaca tcacttacgc tgagtacttc
      241 gaaatgtccg ttcggttggc agaagctatg aaacgatatg ggctgaatac aaatcacaga
      301 atcgtcgtat gcagtgaaaa ctctcttcaa ttctttatgc cggtgttggg cgcgttattt
      361 atcggagttg cagttgcgcc cgcgaacgac atttataatg aacgtgaatt gctcaacagt
      421 atgggcattt cgcagcctac cgtggtgttc gtttccaaaa aggggttgca aaaaattttg
      481 aacgtgcaaa aaaagctccc aatcatccaa aaaattatta tcatggattc taaaacggat
      541 taccagggat ttcagtcgat gtacacgttc gtcacatctc atctacctcc cggttttaat
      601 gaatacgatt ttgtgccaga gtccttcgat agggacaaga caattgcact gatcatgaac
      661 tcctctggat ctactggtct gcctaaaggt gtcgctctgc ctcatagaac tgcctgcgtg
      721 agattctcgc atgccagaga tcctattttt ggcaatcaaa tcattccgga tactgcgatt
      781 ttaagtgttg ttccattcca tcacggtttt ggaatgttta ctacactcgg atatttgata
      841 tgtggatttc gagtcgtctt aatgtataga tttgaagaag agctgtttct gaggagcctt
      901 caggattaca agattcaaag tgcgctgctg gtgccaaccc tattctcctt cttcgccaaa
      961 agcactctga ttgacaaata cgatttatct aatttacacg aaattgcttc tggtggcgct
     1021 cccctctcta aggaagtcgg ggaagcggtt gccaagaggt tccatctgcc aggtatcagg
     1081 caaggatatg ggctcactga gactacatca gctattctga ttacacccga gggggatgat
     1141 aaaccgggcg cggtcggtaa agttgttcca ttttttgaag cgaaggttgt ggatctggat
     1201 accgggaaaa cgctgggcgt taatcaaaga ggcgaactgt gtgtgagagg tcctatgatt
     1261 atgtccggtt atgtaaacaa tccggaagcg accaacgcct tgattgacaa ggatggatgg
     1321 ctacattctg gagacatagc ttactgggac gaagacgaac acttcttcat cgttgaccgc
     1381 ctgaagtctc tgattaagta caaaggctat caggtggctc ccgctgaatt ggaatccatc
     1441 ttgctccaac accccaacat cttcgacgca ggtgtcgcag gtcttcccga cgatgacgcc
     1501 ggtgaacttc ccgccgccgt tgttgttttg gagcacggaa agacgatgac ggaaaaagag
     1561 atcgtggatt acgtcgccag tcaagtaaca accgcgaaaa agttgcgcgg aggagttgtg
     1621 tttgtggacg aagtaccgaa aggtcttacc ggaaaactcg acgcaagaaa aatcagagag
     1681 atcctcataa aggccaagaa gggcggaaag atctga
//
