LOCUS       sequence-263386-         495 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used as a donor vector to clone into pSLIK.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Pereira EJ, Burns JS, Lee CY, Marohl T, Calderon D, Wang L, Atkins 
            KA, Wang CC, Janes KA
  TITLE     Sporadic activation of an oxidative stress-dependent NRF2-p53 
            signaling network in breast epithelial spheroids and 
            premalignancies.
  JOURNAL   Sci Signal. 2020 Apr 14;13(627). pii: 13/627/eaba4200. doi: 
            10.1126/scisignal.aba4200.
  PUBMED    32291314
REFERENCE   2  (bases 1 to 495)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2020-04-14"; volume: "13(627). pii: 
            13/627/eaba4200. doi"; pages: " 10.1126/scisignal.aba4200"
COMMENT     Sequence Label: pEN_TTmiRc2_BirA_CDKN1A
FEATURES             Location/Qualifiers
     source          1..495
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..495
                     /codon_start=1
                     /gene="Homo sapiens CDKN1A"
                     /product="human cyclin-dependent kinase inhibitor 1A"
                     /label=p21
                     /note="mediates p53-dependent arrest in the G1 phase of the
                     cell cycle"
                     /translation="MSEPAGDVRQNPCGSKACRRLFGPVDSEQLRRDCDALMAGCIQEA
                     RERWNFDFVTETPLEGDFAWERVRGLGLPKLYLPTGPRRGRDELGGGRRPGTSPALLQG
                     TAEEDHVDLSLSCTLVPRSGEQAEGSPGGPGDSQGRKRRQTSMTDFYHSKRRLIFSKRK
                     P"
ORIGIN
        1 atgtcagaac cggctgggga tgtccgtcag aacccatgcg gcagcaaggc ctgccgccgc
       61 ctcttcggcc cagtggacag cgagcagctg agacgcgact gtgatgcgct aatggcgggc
      121 tgcatccagg aggcccgtga gcgatggaac ttcgactttg tcaccgagac accactggag
      181 ggtgacttcg cctgggagcg tgtgcggggc cttggcctgc ccaagctcta ccttcccacg
      241 gggccccggc gaggccggga tgagttggga ggaggcaggc ggcctggcac ctcacctgct
      301 ctgctgcagg ggacagcaga ggaagaccat gtggacctgt cactgtcttg tacccttgtg
      361 cctcgctcag gggagcaggc tgaagggtcc ccaggtggac ctggagactc tcagggtcga
      421 aaacggcggc agaccagcat gacagatttc taccactcca aacgccggct gatcttctcc
      481 aagaggaagc cctaa
//
