LOCUS       sequence-263389-        1716 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used as a donor vector containing N-term 3xFLAG to clone into pSLIK.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Pereira EJ, Burns JS, Lee CY, Marohl T, Calderon D, Wang L, Atkins 
            KA, Wang CC, Janes KA
  TITLE     Sporadic activation of an oxidative stress-dependent NRF2-p53 
            signaling network in breast epithelial spheroids and 
            premalignancies.
  JOURNAL   Sci Signal. 2020 Apr 14;13(627). pii: 13/627/eaba4200. doi: 
            10.1126/scisignal.aba4200.
  PUBMED    32291314
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2020-04-14"; volume: "13(627). pii: 
            13/627/eaba4200. doi"; pages: " 10.1126/scisignal.aba4200"
COMMENT     Sequence Label: pEN_TTmiRc2_3xflag_CHEK2
FEATURES             Location/Qualifiers
     source          1..1716
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             8..73
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 ctagatggac tacaaagacc atgacggtga ttataaagat catgacatcg attacaagga
       61 tgacgatgac aagactagaa tgtctcggga gtcggatgtt gaggctcagc agtctcatgg
      121 cagcagtgcc tgttcacagc cccatggcag cgttacccag tcccaaggct cctcctcaca
      181 gtcccagggc atatccagct cctctaccag cacgatgcca aactccagcc agtcctctca
      241 ctccagctct gggacactga gctccttaga gacagtgtcc actcaggaac tctattctat
      301 tcctgaggac caagaacctg aggaccaaga acctgaggag cctacccctg ccccctgggc
      361 tcgattatgg gcccttcagg atggatttgc caatcttgaa tgtgtgaatg acaactactg
      421 gtttgggagg gacaaaagct gtgaatattg ctttgatgaa ccactgctga aaagaacaga
      481 taaataccga acatacagca agaaacactt tcggattttc agggaagtgg gtcctaaaaa
      541 ctcttacatt gcatacatag aagatcacag tggcaatgga acctttgtaa atacagagct
      601 tgtagggaaa ggaaaacgcc gtcctttgaa taacaattct gaaattgcac tgtcactaag
      661 cagaaataaa gtttttgtct tttttgatct gactgtagat gatcagtcag tttatcctaa
      721 ggcattaaga gatgaataca tcatgtcaaa aactcttgga agtggtgcct gtggagaggt
      781 aaagctggct ttcgagagga aaacatgtaa gaaagtagcc ataaagatca tcagcaaaag
      841 gaagtttgct attggttcag caagagaggc agacccagct ctcaatgttg aaacagaaat
      901 agaaattttg aaaaagctaa atcatccttg catcatcaag attaaaaact tttttgatgc
      961 agaagattat tatattgttt tggaattgat ggaaggggga gagctgtttg acaaagtggt
     1021 ggggaataaa cgcctgaaag aagctacctg caagctctat ttttaccaga tgctcttggc
     1081 tgtgcagtac cttcatgaaa acggtattat acaccgtgac ttaaagccag agaatgtttt
     1141 actgtcatct caagaagagg actgtcttat aaagattact gattttgggc actccaagat
     1201 tttgggagag acctctctca tgagaacctt atgtggaacc cccacctact tggcgcctga
     1261 agttcttgtt tctgttggga ctgctgggta taaccgtgct gtggactgct ggagtttagg
     1321 agttattctt tttatctgcc ttagtgggta tccacctttc tctgagcata ggactcaagt
     1381 gtcactgaag gatcagatca ccagtggaaa atacaacttc attcctgaag tctgggcaga
     1441 agtctcagag aaagctctgg accttgtcaa gaagttgttg gtagtggatc caaaggcacg
     1501 ttttacgaca gaagaagcct taagacaccc gtggcttcag gatgaagaca tgaagagaaa
     1561 gtttcaagat cttctgtctg aggaaaatga atccacagct ctaccccagg ttctagccca
     1621 gccttctact agtcgaaagc ggccccgtga aggggaagcc gagggtgccg agaccacaaa
     1681 gcgcccagct gtgtgtgctg ctgtgttgtg acaatt
//
