LOCUS       sequence-263400-         963 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression vector for an inducible BirA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Pereira EJ, Burns JS, Lee CY, Marohl T, Calderon D, Wang L, Atkins 
            KA, Wang CC, Janes KA
  TITLE     Sporadic activation of an oxidative stress-dependent NRF2-p53 
            signaling network in breast epithelial spheroids and 
            premalignancies.
  JOURNAL   Sci Signal. 2020 Apr 14;13(627). pii: 13/627/eaba4200. doi: 
            10.1126/scisignal.aba4200.
  PUBMED    32291314
REFERENCE   2  (bases 1 to 963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2020-04-14"; volume: "13(627). pii: 
            13/627/eaba4200. doi"; pages: " 10.1126/scisignal.aba4200"
COMMENT     Sequence Label: pSLIK_hygro_BirA
FEATURES             Location/Qualifiers
     source          1..963
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1..351,352..354,355..963)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
ORIGIN
        1 atgaaggaca acaccgtgcc cctgaagctg atcgccctgc tggccaacgg cgagttccac
       61 tctggcgagc agctgggaga gaccctggga atgagcagag ccgccatcaa caagcacatc
      121 cagacactga gagactgggg agtggacgtg ttcaccgtgc ctggcaaggg ctacagcctg
      181 cctgagccta tccagctgct gaacgccaag cagatcctgg gacagctgga tggcggaagc
      241 gtggccgtgc tgcctgtgat cgactccacc aatcagtacc tgctggacag aatcggagag
      301 ctgaagtccg gcgacgcctg catcgccgag taccagcagg ctggcagagg aggcagagga
      361 cggaagtggt tcagcccatt cggagccaac ctgtacctgt ccatgttctg gagactggag
      421 cagggacctg ctgctgccat cggactgagt ctggtgatcg gaatcgtgat ggccgaggtg
      481 ctgagaaagc tgggagccga caaggtgaga gtgaagtggc ctaatgacct gtacctccag
      541 gaccgcaagc tggctggcat cctggtggag ctgacaggca agacaggcga tgccgctcag
      601 atcgtgatcg gagccggaat caacatggcc atgagaagag tggaggagag cgtggtgaac
      661 cagggctgga tcaccctgca ggaggctggc atcaacctgg accggaacac cctggccgcc
      721 atgctgatca gagagctgag agccgctctg gagctgttcg agcaggaggg actggctcct
      781 tacctgagca gatgggagaa gctggacaac ttcatcaaca gacctgtgaa gctgatcatc
      841 ggcgacaagg aaatcttcgg catctccaga ggaatcgaca agcagggagc tctgctgctg
      901 gagcaggacg gaatcatcaa gccctggatg ggcggagaaa tctccctgag aagcgcagag
      961 aag
//
