LOCUS       sequence-26344-e         772 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Boehm JS, Zhao JJ, Yao J, Kim SY, Firestein R, Dunn IF, Sjostrom SK,
            Garraway LA, Weremowicz S, Richardson AL, Greulich H, Stewart CJ, 
            Mulvey LA, Shen RR, Ambrogio L, Hirozane-Kishikawa T, Hill DE, Vidal
            M, Meyerson M, Grenier JK, Hinkle G, Root DE, Roberts TM, Lander ES,
            Polyak K, Hahn WC
  TITLE     Integrative Genomic Approaches Identify IKBKE as a Breast Cancer 
            Oncogene (Kinase Library Clones)
  JOURNAL   Cell. 2007 Jun 15. 129(6):1065-1079.
  PUBMED    17574021
REFERENCE   2  (bases 1 to 772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Jun 15. 129(6):1065-1079."
COMMENT     Sequence Label: pWZL Neo Myr Flag MAP3K6
FEATURES             Location/Qualifiers
     source          1..772
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      42..51
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             48..89
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             114..137
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    144..168
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gccctcactc cttctctagg cgccggccgg atccagaatt cgccaccatg gggtcttcaa
       61 aatctaaacc aaaggacccc agccagcgcc ggcgcaggat ccgaggttac cttgactaca
      121 aagacgatga cgacaagcaa ttgacaagtt tgtacaaaaa agttggcatg aacttgctgc
      181 tctcctaccg cgatgtgcag gactactcgg ccatcattga gctggtggag acgctgcagg
      241 ccttgcccac ctgtgatgtg gccgagcagc ataatgtctg cttccactac acttttgccc
      301 tcaaccggag gaacaggcct ggggaccggg cgaaggccct gtctgtgctg ctgccgctgg
      361 tacagcttga gggctctgtg gcgcccgatc tgtactgcat gtgtggccgt atctacaagg
      421 acatgttctt cagctcgggt ttccaggatg ctgggcaccg ggagcaggcc tatcactggt
      481 atcgcaaggc ttttgacgta gagcccagcc ttcactcagg catcaatgca gctgtgctcc
      541 tcattgctgc cgggcagcac tttgaggatt ccaaagagct ccggctaata ggcatgaagc
      601 tgggctgcct gctggcccgc aaaggctgcg tggagaagat gcagtattac tgggatgtgg
      661 gtttctacct gggagcccag atcctcgcca atgaccccac ccaggtggtg ctggctgcag
      721 agcagctgta taagctcaat gcccccatat ggtacctggt gtccgtgatg ga
//
