LOCUS       sequence-263442-        5163 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Neuron-specific expression of  iCre.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5163)
  AUTHORS   Dupraz S, Hilton BJ, Husch A, Santos TE, Coles CH, Stern S, 
            Brakebusch C, Bradke F
  TITLE     RhoA Controls Axon Extension Independent of Specification in the 
            Developing Brain.
  JOURNAL   Curr Biol. 2019 Nov 18;29(22):3874-3886.e9. doi: 
            10.1016/j.cub.2019.09.040. Epub 2019 Oct 31.
  PUBMED    31679934
REFERENCE   2  (bases 1 to 5163)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2019.09.040"; journalName: "Curr Biol"; date: 
            "2019-11-18- 18"; volume: "29"; issue: "22"; pages: "3874-3886.e9"
COMMENT     Sequence Label: pTalpha1-iCre
FEATURES             Location/Qualifiers
     source          1..5163
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     regulatory      1251..1260
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1263..1283
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(2316..2335)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2381..2436
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2435..2454)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(2797..2813)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2797..2813)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2810..2832)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2821..2837
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2845..2851,2852..2869,2870..2875))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2890..2911
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2970..3165
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3016..3151
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3171..3305
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3208..3227)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3262..3281
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3373..3390)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3544..4132)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3624..3643)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4303..5094,5095..5163))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4926..4945
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct gggtcgacat tgattattga ctagtcaatt ccgtattagg agggatggct
      181 catttctagg gacaaaataa cacaggctct ggggtgaggt agggtcgggt aagggggcga
      241 gcggggaggg gtgatgggag agcactaccg ctaaagaaag caagctctct agtaaagcgt
      301 taagtactac tttgtatatt gtttctcttt tttcctttct ctctctctct ctctttgttt
      361 gtttttggtt tttgagacag gatatcacac tatatatcca ggccgggtac aaaataaccg
      421 cagtcctccg cttcccaatg ctgggattta caggcataaa ccactaaggg cggttatgat
      481 gaccttgagc tgcaagtctt cctgcctctg cctcccgggt gctgttgagg gtcataggcg
      541 tggtctattc atactgagct tctgaatttt gctcaaatat taataataat agtaataata
      601 ataaccacaa taatacaact gtaaaactaa acatttaccc acgccttttt gaccatcatt
      661 cccatagctc ttgctacttt atttaaagcg aacagagatg ttgaatcctg acggaacgta
      721 tttaaattta gtgtagtata aatgaaaagc tggaatttac cataaagaat ctcaacacaa
      781 attctgtgat taagtgttgg ggaaaacctt aaattatcct aactacagtt taaggtttaa
      841 gctccgtata atcacccaac ccccgttttc tttcttccct ctctacccct ccccagctcc
      901 accccataat ggatgctcgg ctagttgctt ttgcgaggcc tttgtctgaa ggatgcaaaa
      961 tctacggatg ctagcgaggg ggggaagggg ggagagatta cctcatacca tgtcgcttgc
     1021 accaatcacc actctcctgt cgcggcttct ctgggcagac ggaggggtct ggaccaacag
     1081 gaaaaggcct tggcccatcc ccatggtgac cgagctgtat ataaggaggc gcatccgccc
     1141 aagtgccgca ggttctctta catcgaccgc ttaagagtcg cgctgtaaga agcaacacct
     1201 cctcctcgcc tccgccatcc acccggcagc cgcgaagcag caacgaattc gccaccatgg
     1261 tgcccaagaa gaagaggaaa gtctccaacc tgctgactgt gcaccaaaac ctgcctgccc
     1321 tccctgtgga tgccacctct gatgaagtca ggaagaacct gatggacatg ttcagggaca
     1381 ggcaggcctt ctctgaacac acctggaaga tgctcctgtc tgtgtgcaga tcctgggctg
     1441 cctggtgcaa gctgaacaac aggaaatggt tccctgctga acctgaggat gtgagggact
     1501 acctcctgta cctgcaagcc agaggcctgg ctgtgaagac catccaacag cacctgggcc
     1561 agctcaacat gctgcacagg agatctggcc tgcctcgccc ttctgactcc aatgctgtgt
     1621 ccctggtgat gaggagaatc agaaaggaga atgtggatgc tggggagaga gccaagcagg
     1681 ccctggcctt tgaacgcact gactttgacc aagtcagatc cctgatggag aactctgaca
     1741 gatgccagga catcaggaac ctggccttcc tgggcattgc ctacaacacc ctgctgcgca
     1801 ttgccgaaat tgccagaatc agagtgaagg acatctcccg caccgatggt gggagaatgc
     1861 tgatccacat tggcaggacc aagaccctgg tgtccacagc tggtgtggag aaggccctgt
     1921 ccctgggggt taccaagctg gtggagagat ggatctctgt gtctggtgtg gctgatgacc
     1981 ccaacaacta cctgttctgc cgggtcagaa agaatggtgt ggctgcccct tctgccacct
     2041 cccaactgtc cacccgggcc ctggaaggga tctttgaggc cacccaccgc ctgatctatg
     2101 gtgccaagga tgactctggg cagagatacc tggcctggtc tggccactct gccagagtgg
     2161 gtgctgccag ggacatggcc agggctggtg tgtccatccc tgaaatcatg caggctggtg
     2221 gctggaccaa tgtgaacatt gtgatgaact acatcagaaa cctggactct gagactgggg
     2281 ccatggtgag gctgctcgag gatggggact gaagatcttt ttccctctgc caaaaattat
     2341 ggggacatca tgaagcccct tgagcatctg acttctggct aataaaggaa atttattttc
     2401 attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca
     2461 aatcatttaa aacatcagaa tgagtatttg gtttagagtt tggcaacata tgcccatatg
     2521 ctggctgcca tgaacaaagg ttggctataa agaggtcatc agtatatgaa acagccccct
     2581 gctgtccatt ccttattcca tagaaaagcc ttgacttgag gttagatttt ttttatattt
     2641 tgttttgtgt tatttttttc tttaacatcc ctaaaatttt ccttacatgt tttactagcc
     2701 agatttttcc tcctctcctg actactccca gtcatagctg tccctcttct cttatggaga
     2761 tccctcgacc tgcagcccaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     2821 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     2881 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     2941 gtcgggaaac ctgtcgtgcc agcggatccg catctcaatt agtcagcaac catagtcccg
     3001 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     3061 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     3121 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct aacttgttta
     3181 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     3241 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     3301 ggatccgctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     3361 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     3421 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     3481 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     3541 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     3601 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     3661 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     3721 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     3781 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     3841 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     3901 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     3961 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     4021 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     4081 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     4141 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     4201 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     4261 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     4321 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     4381 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     4441 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     4501 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     4561 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     4621 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     4681 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     4741 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     4801 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     4861 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     4921 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     4981 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     5041 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     5101 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     5161 cat
//
