LOCUS       sequence-263445-        5193 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Neuron-specific expression of  Dre.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5193)
  AUTHORS   Dupraz S, Hilton BJ, Husch A, Santos TE, Coles CH, Stern S, 
            Brakebusch C, Bradke F
  TITLE     RhoA Controls Axon Extension Independent of Specification in the 
            Developing Brain.
  JOURNAL   Curr Biol. 2019 Nov 18;29(22):3874-3886.e9. doi: 
            10.1016/j.cub.2019.09.040. Epub 2019 Oct 31.
  PUBMED    31679934
REFERENCE   2  (bases 1 to 5193)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2019.09.040"; journalName: "Curr Biol"; date: 
            "2019-11-18- 18"; volume: "29"; issue: "22"; pages: "3874-3886.e9"
COMMENT     Sequence Label: pTalpha1-Dre
FEATURES             Location/Qualifiers
     source          1..5193
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1155..1175
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1176..1202
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1209..2231
                     /product="site-specific recombinase"
                     /label=Dre recombinase
                     /note="Dre is a site-specific recombinase from 
                     bacteriophage D6. Recombination occurs at rox sequences."
     primer_bind     complement(2241..2260)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2306..2361
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2360..2379)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(2722..2738)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2722..2738)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2735..2757)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2746..2762
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2770..2776,2777..2794,2795..2800))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2815..2836
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2895..3090
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2941..3076
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3096..3230
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3133..3152)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3187..3206
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3298..3315)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3469..4057)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3549..3568)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4228..5019,5020..5088))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4851..4870
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5089..5193)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacatttccc cgaaaagtgc cacctgggtc gacattgatt attgactagt caattccgta
       61 ttaggaggga tggctcattt ctagggacaa aataacacag gctctggggt gaggtagggt
      121 cgggtaaggg ggcgagcggg gaggggtgat gggagagcac taccgctaaa gaaagcaagc
      181 tctctagtaa agcgttaagt actactttgt atattgtttc tcttttttcc tttctctctc
      241 tctctctctt tgtttgtttt tggtttttga gacaggatat cacactatat atccaggccg
      301 ggtacaaaat aaccgcagtc ctccgcttcc caatgctggg atttacaggc ataaaccact
      361 aagggcggtt atgatgacct tgagctgcaa gtcttcctgc ctctgcctcc cgggtgctgt
      421 tgagggtcat aggcgtggtc tattcatact gagcttctga attttgctca aatattaata
      481 ataatagtaa taataataac cacaataata caactgtaaa actaaacatt tacccacgcc
      541 tttttgacca tcattcccat agctcttgct actttattta aagcgaacag agatgttgaa
      601 tcctgacgga acgtatttaa atttagtgta gtataaatga aaagctggaa tttaccataa
      661 agaatctcaa cacaaattct gtgattaagt gttggggaaa accttaaatt atcctaacta
      721 cagtttaagg tttaagctcc gtataatcac ccaacccccg ttttctttct tccctctcta
      781 cccctcccca gctccacccc ataatggatg ctcggctagt tgcttttgcg aggcctttgt
      841 ctgaaggatg caaaatctac ggatgctagc gaggggggga aggggggaga gattacctca
      901 taccatgtcg cttgcaccaa tcaccactct cctgtcgcgg cttctctggg cagacggagg
      961 ggtctggacc aacaggaaaa ggccttggcc catccccatg gtgaccgagc tgtatataag
     1021 gaggcgcatc cgcccaagtg ccgcaggttc tcttacatcg accgcttaag agtcgcgctg
     1081 taagaagcaa cacctcctcc tcgcctccgc catccacccg gcagccgcga agcagcaacg
     1141 aattcgccac catgcctaag aagaagagga aggtttaccc atacgatgtt ccagattacg
     1201 ctggatcctc tgagctgatt attagtggtt catctggtgg attcctgcga aacatcggca
     1261 aagagtatca ggaggccgct gaaaacttca tgaggtttat gaatgaccag ggggcgtacg
     1321 ctcctaacac tttgagggat ttgaggttgg tctttcatag ctgggccaga tggtgccatg
     1381 ctcggcagct tgcatggttt ccaattagtc ctgaaatggc acgcgaatac tttcttcagt
     1441 tgcacgatgc agacctggcc tccactacca tcgacaagca ctatgctatg cttaatatgc
     1501 ttctgtccca ctgcggactg ccacccttgt ccgacgacaa gtcagtgagt cttgccatga
     1561 gaagaattag aagagaagcc gcaaccgaaa agggtgagag gacaggacag gcaatccccc
     1621 tgcgctggga cgacctgaag ctgctggatg tgctgctcag caggagcgag cggctggtcg
     1681 acctgcgcaa cagggctttc ctgttcgtag cctataacac cctcatgaga atgtctgaaa
     1741 tatcacgcat cagggttggg gacttggatc agacaggaga cacagtgacc ctgcacatca
     1801 gtcacactaa gacaatcacc acagctgcgg gccttgacaa agtgctctcc cggcgaacca
     1861 cagcagtgct caatgactgg ctggacgtca gtgggcttag agaacatcca gacgctgtgc
     1921 tcttcccacc tatacaccgg tcaaacaaag cccgcattac taccacgccc ctgaccgccc
     1981 ctgccatgga gaagattttc agtgatgcct gggtgctgct gaacaaacgg gacgccaccc
     2041 ccaataaagg gaggtatagg acctggaccg gccattccgc cagggtgggt gccgcaatag
     2101 acatggccga gaaacaggtg tctatggtcg agattatgca ggaagggaca tggaagaagc
     2161 ctgaaacact gatgcggtat ctcagaaggg gcggagtgtc cgtgggagcc aattctcgac
     2221 tgatggatag ctgagtcaga tctttttccc tctgccaaaa attatgggga catcatgaag
     2281 ccccttgagc atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt
     2341 tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca tttaaaacat
     2401 cagaatgagt atttggttta gagtttggca acatatgccc atatgctggc tgccatgaac
     2461 aaaggttggc tataaagagg tcatcagtat atgaaacagc cccctgctgt ccattcctta
     2521 ttccatagaa aagccttgac ttgaggttag atttttttta tattttgttt tgtgttattt
     2581 ttttctttaa catccctaaa attttcctta catgttttac tagccagatt tttcctcctc
     2641 tcctgactac tcccagtcat agctgtccct cttctcttat ggagatccct cgacctgcag
     2701 cccaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     2761 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     2821 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     2881 gtgccagcgg atccgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     2941 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     3001 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     3061 ggcttttttg gaggcctagg cttttgcaaa aagctaactt gtttattgca gcttataatg
     3121 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     3181 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cgctgcatta
     3241 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     3301 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     3361 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     3421 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     3481 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     3541 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     3601 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     3661 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     3721 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     3781 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     3841 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     3901 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     3961 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     4021 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     4081 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     4141 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     4201 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     4261 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     4321 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     4381 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     4441 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     4501 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     4561 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     4621 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     4681 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     4741 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     4801 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     4861 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     4921 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     4981 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     5041 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     5101 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     5161 tatttagaaa aataaacaaa taggggttcc gcg
//
