LOCUS       sequence-263446-        9874 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dox-inducible polycistronic reprogramming lentiviral vector 
            expressing mouse Oct4, Sox2, Klf4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9874)
  AUTHORS   Velychko S, Adachi K, Kim KP, Hou Y, MacCarthy CM, Wu G, Scholer HR
  TITLE     Excluding Oct4 from Yamanaka Cocktail Unleashes the Developmental 
            Potential of iPSCs.
  JOURNAL   Cell Stem Cell. 2019 Oct 30. pii: S1934-5909(19)30423-0. doi: 
            10.1016/j.stem.2019.10.002.
  PUBMED    31708402
REFERENCE   2  (bases 1 to 9874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2019 Oct 30. pii: S1934-5909(19)30423-0. doi: 
            10.1016/j.stem.2019.10.002."
COMMENT     Sequence Label: pHAGE2-TetOminiCMV-OSK
FEATURES             Location/Qualifiers
     source          1..9874
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2251..2269,2270..2292,2293..2311,2312..2334,2335..2353,
                     2354..2376,2377..2395,2396..2418,2419..2437,2438..2460,
                     2461..2479,2480..2502,2503..2521)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2554..2592
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2589..2613
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2679..2688
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3756..3812
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4791..4844
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    6316..6904
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6369..6389)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6787..6798)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7052..7232
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7279..7297)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7365..7469
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7470..7538,7539..8330)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7688..7707)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8501..9089
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9243..9260
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9500..9521
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9536..9541,9542..9559,9560..9566)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9574..9590
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9579..9601
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9598..9614
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9598..9614
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9664..9683
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcca
     2221 caccctaact gacacactcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     2281 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
     2341 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt
     2401 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
     2461 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
     2521 aaaagtgaaa gtcgagctcg gtacccgggt cgaggtaggc gtgtacggtg ggaggcctat
     2581 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
     2641 gacctccata gaagacaccg ggaccgatcc agcctgcggc cgccatggct ggacacctgg
     2701 cttcagactt cgccttctca cccccaccag gtgggggtga tgggtcagca gggctggagc
     2761 cgggctgggt ggatcctcga acctggctaa gcttccaagg gcctccaggt gggcctggaa
     2821 tcggaccagg ctcagaggta ttggggatct ccccatgtcc gcccgcatac gagttctgcg
     2881 gagggatggc atactgtgga cctcaggttg gactgggcct agtcccccaa gttggcgtgg
     2941 agactttgca gcctgagggc caggcaggag cacgagtgga aagcaactca gagggaacct
     3001 cctctgagcc ctgtgccgac cgccccaatg ccgtgaagtt ggagaaggtg gaaccaactc
     3061 ccgaggagtc ccaggacatg aaagccctgc agaaggagct agaacagttt gccaagctgc
     3121 tgaagcagaa gaggatcacc ttggggtaca cccaggccga cgtggggctc accctgggcg
     3181 ttctctttgg aaaggtgttc agccagacca ccatctgtcg cttcgaggcc ttgcagctca
     3241 gccttaagaa catgtgtaag ctgcggcccc tgctggagaa gtgggtggag gaagccgaca
     3301 acaatgagaa ccttcaggag atatgcaaat cggagaccct ggtgcaggcc cggaagagaa
     3361 agcgaactag cattgagaac cgtgtgaggt ggagtctgga gaccatgttt ctgaagtgcc
     3421 cgaagccctc cctacagcag atcactcaca tcgccaatca gcttgggcta gagaaggatg
     3481 tggttcgagt atggttctgt aaccggcgcc agaagggcaa aagatcaagt attgagtatt
     3541 cccaacgaga agagtatgag gctacaggga cacctttccc agggggggct gtatcctttc
     3601 ctctgccccc aggtccccac tttggcaccc caggctatgg aagcccccac ttcaccacac
     3661 tctactcagt cccttttcct gagggcgagg cctttccctc tgttcccgtc actgctctgg
     3721 gctctcccat gcattcaaac gctagcggca gcggcgccac aaacttctct ctgctaaagc
     3781 aagcaggtga tgttgaagaa aaccccgggc ctgcatgcat gtataacatg atggagacgg
     3841 agctgaagcc gccgggcccg cagcaagctt cggggggcgg cggcggagga ggcaacgcca
     3901 cggcggcggc gaccggcggc aaccagaaga acagcccgga ccgcgtcaag aggcccatga
     3961 acgccttcat ggtatggtcc cgggggcagc ggcgtaagat ggcccaggag aaccccaaga
     4021 tgcacaactc ggagatcagc aagcgcctgg gcgcggagtg gaaacttttg tccgagaccg
     4081 agaagcggcc gttcatcgac gaggccaagc ggctgcgcgc tctgcacatg aaggagcacc
     4141 cggattataa ataccggccg cggcggaaaa ccaagacgct catgaagaag gataagtaca
     4201 cgcttcccgg aggcttgctg gcccccggcg ggaacagcat ggcgagcggg gttggggtgg
     4261 gcgccggcct gggtgcgggc gtgaaccagc gcatggacag ctacgcgcac atgaacggct
     4321 ggagcaacgg cagctacagc atgatgcagg agcagctggg ctacccgcag cacccgggcc
     4381 tcaacgctca cggcgcggca cagatgcaac cgatgcaccg ctacgacgtc agcgccctgc
     4441 agtacaactc catgaccagc tcgcagacct acatgaacgg ctcgcccacc tacagcatgt
     4501 cctactcgca gcagggcacc cccggtatgg cgctgggctc catgggctct gtggtcaagt
     4561 ccgaggccag ctccagcccc cccgtggtta cctcttcctc ccactccagg gcgccctgcc
     4621 aggccgggga cctccgggac atgatcagca tgtacctccc cggcgccgag gtgccggagc
     4681 ccgctgcgcc cagtagactg cacatggccc agcactacca gagcggcccg gtgcccggca
     4741 cggccattaa cggcacactg cccctgtcgc acatggcatg cggctccggc gagggcaggg
     4801 gaagtcttct aacatgcggg gacgtggagg aaaatcccgg cccactcgag atgaggcagc
     4861 cacctggcga gtctgacatg gctgtcagcg acgctctgct cccgtccttc tccacgttcg
     4921 cgtccggccc ggcgggaagg gagaagacac tgcgtccagc aggtgccccg actaaccgtt
     4981 ggcgtgagga actctctcac atgaagcgac ttcccccact tcccggccgc ccctacgacc
     5041 tggcggcgac ggtggccaca gacctggaga gtggcggagc tggtgcagct tgcagcagta
     5101 acaacccggc cctcctagcc cggagggaga ccgaggagtt caacgacctc ctggacctag
     5161 actttatcct ttccaactcg ctaacccacc aggaatcggt ggccgccacc gtgaccacct
     5221 cggcgtcagc ttcatcctcg tcttccccag cgagcagcgg ccctgccagc gcgccctcca
     5281 cctgcagctt cagctatccg atccgggccg ggggtgaccc gggcgtggct gccagcaaca
     5341 caggtggagg gctcctctac agccgagaat ctgcgccacc tcccacggcc cccttcaacc
     5401 tggcggacat caatgacgtg agcccctcgg gcggcttcgt ggctgagctc ctgcggccgg
     5461 agttggaccc agtatacatt ccgccacagc agcctcagcc gccaggtggc gggctgatgg
     5521 gcaagtttgt gctgaaggcg tctctgacca cccctggcag cgagtacagc agcccttcgg
     5581 tcatcagtgt tagcaaagga agcccagacg gcagccaccc cgtggtagtg gcgccctaca
     5641 gcggtggccc gccgcgcatg tgccccaaga ttaagcaaga ggcggtcccg tcctgcacgg
     5701 tcagccggtc cctagaggcc catttgagcg ctggacccca gctcagcaac ggccaccggc
     5761 ccaacacaca cgacttcccc ctggggcggc agctccccac caggactacc cctacactga
     5821 gtcccgagga actgctgaac agcagggact gtcaccctgg cctgcctctt cccccaggat
     5881 tccatcccca tccggggccc aactaccctc ctttcctgcc agaccagatg cagtcacaag
     5941 tcccctctct ccattatcaa gagctcatgc caccgggttc ctgcctgcca gaggagccca
     6001 agccaaagag gggaagaagg tcgtggcccc ggaaaagaac agccacccac acttgtgact
     6061 atgcaggctg tggcaaaacc tataccaaga gttctcatct caaggcacac ctgcgaactc
     6121 acacaggcga gaaaccttac cactgtgact gggacggctg tgggtggaaa ttcgcccgct
     6181 ccgatgaact gaccaggcac taccgcaaac acacagggca ccggcccttt cagtgccaga
     6241 agtgcgacag ggccttttcc aggtcggacc accttgcctt acacatgaag aggcactttt
     6301 aaatcgatag atcctaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     6361 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     6421 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     6481 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     6541 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     6601 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     6661 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     6721 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     6781 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     6841 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     6901 ccgcctggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     6961 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atcacctgca
     7021 ggacaggcgc gccctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     7081 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     7141 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     7201 acccttttag tcagtgtgga aaatctctag cacccgggcg attaaggaaa gggctagatc
     7261 attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     7321 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     7381 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     7441 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     7501 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     7561 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     7621 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     7681 aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc
     7741 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     7801 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     7861 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     7921 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     7981 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     8041 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     8101 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     8161 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     8221 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     8281 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     8341 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     8401 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     8461 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     8521 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     8581 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     8641 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     8701 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     8761 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     8821 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     8881 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     8941 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     9001 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     9061 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     9121 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     9181 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     9241 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     9301 cgcgttggcc gattcattaa tgcagcaagc tcatggctga ctaatttttt ttatttatgc
     9361 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     9421 aggcctaggc ttttgcaaaa agctccccgt ggcacgacag gtttcccgac tggaaagcgg
     9481 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     9541 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     9601 gaaacagcta tgacatgatt acgaatttca caaataaagc atttttttca ctgcattcta
     9661 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc
     9721 aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc
     9781 tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt
     9841 gtatttgtta aatatgtact acaaacttag tagt
//
