LOCUS       sequence-263455-        3981 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  full-length of foxc1a without stop codon in pmE Gateway vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Shin M, Nozaki T, Idrizi F, Isogai S, Ogasawara K, Ishida K, Yuge S,
            Roscoe B, Wolfe SA, Fukuhara S, Mochizuki N, Deguchi T, Lawson ND
  TITLE     Valves Are a Conserved Feature of the Zebrafish Lymphatic System.
  JOURNAL   Dev Cell. 2019 Nov 4;51(3):374-386.e5. doi: 
            10.1016/j.devcel.2019.08.019. Epub 2019 Sep 26.
  PUBMED    31564611
REFERENCE   2  (bases 1 to 3981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2019.08.019"; journalName: "Dev Cell"; date: 
            "2019-11-4- 4"; volume: "51"; issue: "3"; pages: "374-386.e5"
COMMENT     Sequence Label: pME-foxc1a
FEATURES             Location/Qualifiers
     source          1..3981
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(144..171)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(263..349)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     398..420
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     412..429
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     413..429
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    445..544
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1982..2081)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2098..2117)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2099..2117)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2122..2138)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2122..2138)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2240..2259)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2251..3060
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2318..2337)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3207..3795
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3696..3715
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3949..3966
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
       61 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc
      121 caggaagagt ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt
      181 gatgcctggc agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa
      241 cgttcaaatc cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac
      301 agataaaacg aaaggcccag tcttccgact gagcctttcg ttttatttga tgcctggcag
      361 ttccctactc tcgcgttaac gctagcatgg atgttttccc agtcacgacg ttgtaaaacg
      421 acggccagtc ttaagctcgg gccccaaata atgattttat tttgactgat agtgacctgt
      481 tcgttgcaac aaattgatga gcaatgcttt tttataatgc caactttgta caaaaaagca
      541 ggctccgcca ccatgcaggc gcgctattcc gtctccagtc ccaactcgct gggagttgtg
      601 ccgtacatca gcagcgacca aagttattac cgagccgccg ccggaggggg gtacaccggc
      661 atgccagccc ccatgaccat gtactctcac gcggcccacg accagtaccc cgccagcatg
      721 gcgagggcat atgggccata cactccacag ccgcagccta aggatatggt gaaacccccg
      781 tacagctaca ttgcgctcat cacaatggca atccagaact ccccggacaa aaaggtgacc
      841 cttaatggta tttatcagtt catcatggag cgctttccct tttacagaga caacaagcaa
      901 ggctggcaga acagcatccg acataattta tccctgaacg agtgctttgt caaagttcca
      961 cgcgatgata agaaacccgg aaaaggcagc tactggaccc tcgacccgga ctcgtataat
     1021 atgtttgaga acggcagctt tttgaggcga cgacggcgat ttaaaaagaa ggacgccatg
     1081 aaggacaagg aggaccgagg tgttaaagag gcgccgtccc gccaggcgca gccgcaggcc
     1141 cgcgagcagg agcagtcggt gcctggctct cagcccgtgc gcattcagga cattaaaacc
     1201 gagaacggca cgtccactcc tccgcaggct gtttcgccca cgctaagcac cgtcccgaag
     1261 atcgagagcc ccgacagcag cagcagcatg tcgagcggca gcccgcacag catcccttcc
     1321 acccgctccc tcagcctgga tagcgctgga gagcagcacc accaggcccc cgcgcagggc
     1381 ttcagcgtgg acaacatcat gacctccctc cgcggatcgc cgcacagctc cggagagctc
     1441 accccgtctc tcgtggctcc ctcgcggaca ggtataacac ccacgttgtc cctgaattat
     1501 tctcccaatc agtcgtccgt gtacagctcg ccctgcagcc agaactctat ctcgactacc
     1561 tccaacgcca cctatcactg caacatgcag gccatgagct tgtacgcggg cggagatcgc
     1621 tcgggccact tggggaccac cgctaccacc gtggacgaaa ctctgcctga ttactcgatc
     1681 acgaccacca cgtcgtcgtt gagccacggg aatctgagct cggcccagga gggccaccat
     1741 ccgcaccaag ggcgcctcgc ctcatggtac ctgaaccagg ccggagacat tggccatcta
     1801 ggcgcgacgt acccagcgca gcagcagaac ttccattcgg ttcgagagat gttcgaatcg
     1861 cagagaatcg gtctgaataa ttcgccggta aacgggaata acagctgtca aatgtcgttt
     1921 cccccgagcc agcccattta ccgcacgtcg ggggcttttg tgtatgactg cagcaaattt
     1981 cacccagctt tcttgtacaa agttggcatt ataagaaagc attgcttatc aatttgttgc
     2041 aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccagc tgatatcccc
     2101 tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa
     2161 aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct
     2221 gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcgagg
     2281 ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat
     2341 gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg
     2401 tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta
     2461 aactggctga cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat
     2521 gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt attagaagaa
     2581 tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat
     2641 tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg
     2701 caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc
     2761 tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca
     2821 gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata
     2881 ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta
     2941 tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt
     3001 attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa
     3061 tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc
     3121 gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac
     3181 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     3241 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     3301 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     3361 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     3421 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     3481 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     3541 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     3601 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     3661 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     3721 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     3781 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     3841 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     3901 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     3961 agcgaggaag cggaagagcg c
//
