LOCUS       sequence-263500-       10649 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of G protein-coupled receptors for PRESTO-Tango: parallel
            receptorome expression and screening via transcriptional output, 
            with transcriptional activation following arrestin translocation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10649)
  AUTHORS   Kroeze WK, Sassano MF, Huang XP, Lansu K, McCorvy JD, Giguere PM, 
            Sciaky N, Roth BL
  TITLE     PRESTO-Tango as an open-source resource for interrogation of the 
            druggable human GPCRome.
  JOURNAL   Nat Struct Mol Biol. 2015 May;22(5):362-9. doi: 10.1038/nsmb.3014. 
            Epub 2015 Apr 20.
  PUBMED    25895059
REFERENCE   2  (bases 1 to 10649)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.3014";
            journalName: "Nat Struct Mol Biol"; date: "2015-05"; volume: "22"; 
            issue: "5"; pages: "362-9"
COMMENT     Sequence Label: GPR124-Tango
FEATURES             Location/Qualifiers
     source          1..10649
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(45..64)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        236..615
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..819
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     770..790
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     864..883
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        864..882
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(5208..5822,5823..6209)
                     /codon_start=1
                     /product="tetracycline-controlled transactivator, 
                     comprising a fusion of the tetracycline repressor TetR with
                     the C-terminal activation domain of herpes simplex virus 
                     VP16"
                     /label=tTA
                     /note="In the Tet-Off(R) system, tTA binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="RLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHVKN
                     KRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTRPT
                     EKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTTDS
                     MPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGSAYSRARTKNNYGSTIEG
                     LLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSLGDELHLDGEDVAMAHADA
                     LDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     primer_bind     complement(5275..5294)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     primer_bind     complement(6241..6258)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6247..6471
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6517..6945
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6604..6623)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6814..6835
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6954..6974)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6959..7288
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7139..7274
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7201..7220
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7355..8149
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7409..7428)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8019..8038
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8323..8444
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8360..8379)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8414..8433
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8493..8509)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8493..8509)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8506..8528)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8517..8533
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8541..8547,8548..8565,8566..8571))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8586..8607
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8724..8741)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8895..9483)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8975..8994)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9654..10445,10446..10514))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10277..10296
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10515..10619)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat
       61 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc
      121 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg
      181 cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat
      241 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
      301 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
      361 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
      421 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
      481 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
      541 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
      601 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
      661 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
      721 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
      781 ggtaggcgtg tacggtggga ggtctatata agcagagctc tctggctaac tagagaaccc
      841 actgcttact ggcttatcga aattaatacg actcactata gggagaccca agctggctag
      901 cgtttaaact taagcttggt accgagctcg gatccactag tccagtgtgg tggaattctg
      961 cagatatcca gcacagtggc ggccgcgcca ccatgaagac gatcatcgcc ctgagctaca
     1021 tcttctgcct ggtattcgcc gactacaagg acgatgatga cgccagcatc gatatgggtg
     1081 ccggtggtag acggatgcgg ggcgccccgg cacgcctgct gctccccctg ctgccttggc
     1141 tgttgctgct cctcgcgccg gaagccaggg gggccccggg atgccctctc tcaataaggt
     1201 cctgcaaatg cagcggtgaa agacctaaag gcctgagcgg cggtgtgcct gggcccgctc
     1261 gccgccgggt tgtgtgcagc ggtggcgatc tgcctgagcc ccctgagccc ggactgttgc
     1321 ctaacggaac agtgactctg ctgctgtcta acaataaaat caccgggctc cggaacgggt
     1381 cattcctggg actttccctc ctcgaaaaac ttgacctgag aaataatatt atcagcacgg
     1441 tacagcctgg tgcctttctg ggtcttggag agcttaagcg actcgacctt agcaacaata
     1501 gaatcggatg tctcacctca gaaacttttc aaggactgcc tagactgctc agactgaata
     1561 tctcaggaaa catcttcagc tccctccagc ccggcgtgtt cgacgagctc cctgccttga
     1621 aagtagttga tctgggcact gaattcctca catgtgattg tcacctgcga tggctcctgc
     1681 catgggccca gaatcgaagt ttgcagctga gtgagcatac tctgtgcgct tacccgagcg
     1741 cccttcatgc ccaggcgctt ggatccctcc aagaggctca gctgtgttgc gaaggggccc
     1801 tggaacttca tacgcaccat ctgataccct cactgagaca agtcgtgttt cagggcgata
     1861 gacttccttt ccagtgtagc gctagttatc tgggtaatga cacacggatc aggtggtacc
     1921 acaaccgagc acccgttgag ggagacgagc aagccgggat cctgctcgcg gaaagcctga
     1981 tccacgactg tactttcatc acaagtgagc tgacactgag tcacatcggg gtgtgggcat
     2041 ccggagagtg ggagtgcacc gtctctatgg cccagggtaa cgcatccaag aaggtggaga
     2101 tcgtggtgtt ggagacaagt gcttcatatt gcccagcaga gcgggtcgct aacaatcgcg
     2161 gagacttcag gtggccaaga acgctggctg ggataacggc ttatcagagt tgcctccagt
     2221 acccgttcac ttccgtgcca ctgggaggcg gcgccccagg aaccagggct agtcggaggt
     2281 gtgaccgggc tggacggtgg gaacccggtg attatagcca ctgtttgtac acaaatgaca
     2341 ttactagagt gctctataca ttcgttctca tgcctataaa tgccagcaac gcactcaccc
     2401 tcgctcacca gctccgggtt tacaccgcgg aggctgctag tttttccgac atgatggacg
     2461 tagtttacgt ggcacagatg atccagaagt ttttgggcta cgtggaccaa attaaagaac
     2521 ttgtggaagt catggtagac atggcttcta atctgatgct ggttgacgag catctcctgt
     2581 ggctcgcaca gagagaagat aaggcctgta gccggattgt gggggccctg gagcgaatag
     2641 ggggtgctgc cctgagtcct catgctcagc acatttcagt gaacgcccga aatgtggcac
     2701 tggaggctta cttgatcaaa cctcattcct acgtaggcct cacatgcacc gctttccaga
     2761 gacgcgaggg gggcgtcccc gggaccaggc ccggctctcc tggacagaat ccccccccag
     2821 aaccggaacc accagccgac cagcagctca ggttccgatg taccacagga agaccaaacg
     2881 tgtcacttag ttcattccac attaagaatt ccgtcgccct tgccagtatt cagttgcccc
     2941 cgtctctgtt ttcatcactg cctgccgcgc tggcaccacc cgtcccccct gactgtaccc
     3001 tccagctgtt ggtttttcga aatggacgcc tgttccatag ccacagcaac acctcaaggc
     3061 ctggtgccgc tggccccggg aagagacgcg gagtggctac ccctgtcata ttcgcaggga
     3121 cttccggatg tggtgtcggg aacttgaccg agcccgtggc tgtgagcctg cgccactggg
     3181 ccgagggcgc cgaacctgtg gcagcgtggt ggagtcaaga ggggcctggc gaggccgggg
     3241 gatggaccag cgaggggtgt caacttaggt cctcccagcc caacgtgtct gcccttcact
     3301 gccagcatct gggtaatgtg gccgtgctga tggagctgag cgcatttcca cgggaagtgg
     3361 ggggagctgg cgcaggcctg caccccgtag tttatccttg tacagcattg ctgctcctct
     3421 gcctgtttgc aactattatt acctatatcc tgaatcactc ctctattaga gtcagccgga
     3481 aaggatggca catgctgctg aacttgtgtt tccacattgc tatgacctct gccgtctttg
     3541 ccggcggcat cacactgaca aactatcaga tggtctgcca agccgttggt atcactctgc
     3601 attactcttc tctgtccacg ctgctttgga tgggagtaaa agctagagtg ctccataagg
     3661 agctgacatg gcgggctcca ccaccacagg agggagatcc tgccttgcct actccctccc
     3721 caatgttgcg gttttacttg atcgcgggag gaatccccct tatcatatgc gggataactg
     3781 ccgccgtgaa tattcacaac taccgggacc atagtccata ctgctggctc gtctggcgcc
     3841 catccctggg tgcattctat attccagttg ccttgattct gcttatcaca tggatttatt
     3901 ttctgtgcgc tggccttagg ctgagaggcc ctctggctca gaacccaaaa gccgggaata
     3961 gcagggcaag cctcgaagca ggcgaggaac tgcgggggag tacccgcctg cgcgggagtg
     4021 gtcccctgct gtctgacagc ggaagtctgt tggctactgg aagcgcccgg gttgggactc
     4081 ctgggcctcc agaagacgga gactctctgt actcccccgg ggtgcaactt ggtgccctgg
     4141 taacaaccca ttttctgtat ctggccatgt gggcatgtgg agccctggcc gtgagccagc
     4201 gatggctccc cagggtcgtc tgtagctgcc tctacggggt cgccgcttca gccttgggcc
     4261 tgttcgtctt tacccaccac tgcgccagga ggagggacgt gcgcgcctcc tggagggcat
     4321 gttgcccccc agcctcccca gcagcgccac acgcacctcc gagggcactg ccagccgccg
     4381 cagaagacgg cagccctgtc ttcggagagg gacccccatc cctgaaaagc tcccccagtg
     4441 gtagctccgg gcaccccctc gccctgggcc cctgcaagct cacgaacctc cagctggcac
     4501 aaagtcaggt ctgtgaagca ggcgcagcag ccggagggga gggagagccc gagcccgctg
     4561 gcacccgggg gaacctggct catagacacc cgaacaatgt gcatcacggt cggagagccc
     4621 ataagagcag agccaagggc cacagggcag gtgaagcctg tggcaaaaat agactgaagg
     4681 ccttgcgagg aggggccgct ggagccctcg aattgctgtc ttctgaatca ggtagcttgc
     4741 acaattcacc tacggattct tatcttgggt caagccgcaa cagtcctgga gcgggtctcc
     4801 aacttgaagg ggaacccatg ctcacaccat ccgagggaag cgacacaagt gcagctcccc
     4861 tcagcgaggc gggtagagca ggacaacggc gctcagcctc cagagacagt ctgaaaggag
     4921 gcggagcact ggagaaggag agtcatagac ggagttatcc acttaacgct gcgtctctga
     4981 atggagctcc taagggtgga aagtacgacg acgtgacact gatgggcgcc gaggtggcct
     5041 ccggcgggtg catgaaaaca ggactctgga agtccgagac cactgtgatc gataccggtg
     5101 gacgcacccc acccagcctg ggtccccaag atgagtcctg caccaccgcc agctcctccc
     5161 tggccaagga cacttcatcg accggtgaga acctgtactt ccagctaaga ttagataaaa
     5221 gtaaagtgat taacagcgca ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa
     5281 cccgtaaact cgcccagaag ctaggtgtag agcagcctac attgtattgg catgtaaaaa
     5341 ataagcgggc tttgctcgac gccttagcca ttgagatgtt agataggcac catactcact
     5401 tttgcccttt agaaggggaa agctggcaag attttttacg taataacgct aaaagtttta
     5461 gatgtgcttt actaagtcat cgcgatggag caaaagtaca tttaggtaca cggcctacag
     5521 aaaaacagta tgaaactctc gaaaatcaat tagccttttt atgccaacaa ggtttttcac
     5581 tagagaatgc attatatgca ctcagcgctg tggggcattt tactttaggt tgcgtattgg
     5641 aagatcaaga gcatcaagtc gctaaagaag aaagggaaac acctactact gatagtatgc
     5701 cgccattatt acgacaagct atcgaattat ttgatcacca aggtgcagag ccagccttct
     5761 tattcggcct tgaattgatc atatgcggat tagaaaaaca acttaaatgt gaaagtgggt
     5821 ccgcgtacag ccgcgcgcgt acgaaaaaca attacgggtc taccatcgag ggcctgctcg
     5881 atctcccgga cgacgacgcc cccgaagagg cggggctggc ggctccgcgc ctgtcctttc
     5941 tccccgcggg acacacgcgc agactgtcga cggccccccc gaccgatgtc agcctggggg
     6001 acgagctcca cttagacggc gaggacgtgg cgatggcgca tgccgacgcg ctagacgatt
     6061 tcgatctgga catgttgggg gacggggatt ccccgggtcc gggatttacc ccccacgact
     6121 ccgcccccta cggcgctctg gatatggccg acttcgagtt tgagcagatg tttaccgatg
     6181 cccttggaat tgacgagtac ggtgggtagt ctagagggcc cgtttaaacc cgctgatcag
     6241 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     6301 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     6361 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     6421 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
     6481 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
     6541 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     6601 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     6661 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     6721 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     6781 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     6841 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     6901 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt
     6961 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     7021 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     7081 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     7141 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     7201 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
     7261 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg
     7321 atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc
     7381 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
     7441 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
     7501 caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg
     7561 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
     7621 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
     7681 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
     7741 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
     7801 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
     7861 actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg
     7921 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
     7981 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
     8041 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
     8101 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg
     8161 gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc
     8221 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     8281 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
     8341 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     8401 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
     8461 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     8521 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     8581 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     8641 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     8701 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     8761 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     8821 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     8881 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     8941 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     9001 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     9061 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     9121 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     9181 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     9241 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     9301 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     9361 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     9421 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     9481 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     9541 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     9601 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     9661 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     9721 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     9781 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     9841 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     9901 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     9961 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
    10021 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
    10081 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
    10141 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
    10201 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
    10261 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
    10321 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
    10381 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
    10441 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
    10501 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
    10561 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
    10621 acatttcccc gaaaagtgcc acctgacgt
//
