LOCUS       sequence-263512-        6248 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6248)
  AUTHORS   Zhao B, Ye X, Yu J, Li L, Li W, Li S, Yu J, Lin JD, Wang CY, 
            Chinnaiyan AM, Lai ZC, Guan KL
  TITLE     TEAD mediates YAP-dependent gene induction and growth control.
  JOURNAL   Genes Dev. 2008 Jul 15. 22(14):1962-71.
  PUBMED    18579750
REFERENCE   2  (bases 1 to 6248)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2008 Jul 15. 22(14):1962-71."
COMMENT     Sequence Label: pCMX-Gal4-TEAD2
FEATURES             Location/Qualifiers
     source          1..6248
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(30..52)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     152..171
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        316..695
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        696..899
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     850..870
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     944..963
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        944..962
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             995..1435
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     protein_bind    1451..1475
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2817..2841)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     intron          2981..3046
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             3176..3196
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3468..3602
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3505..3524)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3559..3578
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4060..4194
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4259..4276)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4430..5018)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4510..4529)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5189..5980,5981..6049))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5812..5831
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6050..6154)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6222..6240
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
       61 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
      121 gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg
      181 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc
      241 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
      301 ttacgccagc gcgttgacat tgattattga ctagttatta atagtaatca attacggggt
      361 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
      421 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
      481 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
      541 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
      601 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
      661 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
      721 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
      781 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
      841 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc
      901 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      961 gggagaccca agcttgaagc aagcctcctg aaagatgaag ctactgtctt ctatcgaaca
     1021 agcatgcgat atttgccgac ttaaaaagct caagtgctcc aaagaaaaac cgaagtgcgc
     1081 caagtgtctg aagaacaact gggagtgtcg ctactctccc aaaaccaaaa ggtctccgct
     1141 gactagggca catctgacag aagtggaatc aaggctagaa agactggaac agctatttct
     1201 actgattttt cctcgagaag accttgacat gattttgaaa atggattctt tacaggatat
     1261 aaaagcattg ttaacaggat tatttgtaca agataatgtg aataaagatg ccgtcacaga
     1321 tagattggct tcagtggaga ctgatatgcc tctaacattg agacagcata gaataagtgc
     1381 gacatcatca tcggaagaga gtagtaacaa aggtcaaaga cagttgactg tatcgccgga
     1441 attcccatca acaagtttgt acaaaaaagt tggcatgggg gaaccccggg ctggggccgc
     1501 cctggacgat ggcagcggct ggacgggcag tgaggaaggc agtgaggagg gtaccggcgg
     1561 cagtgagggg gctgggggtg acgggggccc ggatgcagag ggggtgtgga gcccagacat
     1621 tgagcagagc ttccaggagg ccctggccat ctatccaccc tgcggccgcc ggaaaataat
     1681 tttgtctgat gaaggcaaga tgtatggtcg gaatgaactg atcgcccgct acatcaagct
     1741 gagaacgggg aagacccgaa ctcgaaaaca ggtttctagt cacatccagg ttttggcccg
     1801 aaggaaatca agggaaatcc agtccaagtt gaaggaccag gtttccaagg acaaggcttt
     1861 ccagacaatg gcaaccatgt cctctgccca gctcatctcc gcgccttctc tgcaggccaa
     1921 actgggtccc actggtcctc aggcctctga gcttttccag ttttggtctg gaggatctgg
     1981 gcccccctgg aatgttccag atgtgaagcc attctcacag acaccgttca ccttgtcact
     2041 gactccccca tctactgacc tcccagggta cgagcccccc caagccctct cacccctgcc
     2101 cccacctacc ccatcgcccc cagcctggca ggctcggggc ctgggcaccg cccggttgca
     2161 gctggtagag ttctcagcct tcgtggaacc gccagatgca gttgattctt accagaggca
     2221 cctgttcgtg cacatcagcc agcactgccc cagccccgga gcgccgccgc tcgagagtgt
     2281 ggacgtccgg cagatctacg acaaattccc tgagaaaaag ggtggcctcc gagagctata
     2341 tgatcgtggc cccccccatg ccttcttcct ggtcaagttc tgggcggacc tgaactgggg
     2401 cccaagtggt gaggaggcag gggccggtgg cagcatcagc agtggtggct tctacggagt
     2461 gagcagccag tatgagagcc tggaacacat gaccctcacc tgttcctcca aggtctgctc
     2521 ttttggcaag caggtggtgg agaaggtgga gacggaacgg gcccagctgg aggacggcag
     2581 atttgtgtac cgcctgctgc gctcgcccat gtgcgagtac ctggtgaatt tcttgcacaa
     2641 gttgcggcag ctgcctgagc gatacatgat gaacagcgtc ctggaaaact tcaccatcct
     2701 ccaggtggtg acaaacagag acacccagga actgctgctc tgcaccgcct atgtcttcga
     2761 ggtctccacc agcgagcgtg gggcccagca tcacatttac cgcctggtca gggactgccc
     2821 aactttcttg tacaaagtgg ttgatgggga tccgtcgacg gtaccagata tcaggatcct
     2881 ggccagctag ctaggtagct agaggatctt tgtgaaggaa ccttacttct gtggtgtgac
     2941 ataattggac aaactaccta cagagattta aagctctaag gtaaatataa aatttttaag
     3001 tgtataatgt gttaaactac tgattctaat tgtttgtgta ttttagattc caacctatgg
     3061 aactgatgaa tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga
     3121 agaaatgcca tctagtgatg atgaggctac tgctgactct caacattcta ctcctccaaa
     3181 aaagaagaga aaggtagaag accccaagga ctttccttca gaattgctaa gttttttgag
     3241 tcatgctgtg tttagtaata gaactcttgc ttgctttgct atttacacca caaaggaaaa
     3301 agctgcactg ctatacaaga aaattatgga aaaatatttg atgtatagtg ccttgactag
     3361 agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
     3421 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
     3481 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     3541 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
     3601 tcagcttcag aagatggcgg agggcctcca acacagtaat tttcctcccg actcttaaaa
     3661 tagaaaatgt caagtcagtt aagcaggaag tgactaactg accgcagctg gccgtgcgac
     3721 atcctctttt aattagttgc taggcaactg ccctccagag ggcagtgtgg ttttgcaaga
     3781 ggaagcaaaa agcctctcca cccaggccta gaatgtttcc acccaatcat tactatgaca
     3841 acagctgttt tttttagtat taagcagagg ccgggggccc ctggcctccg cttactctgg
     3901 agaaaaagaa gagaggcatt gtagaggctt ccagaggcaa cttgtcaaaa caggactgct
     3961 tctatttctg tcacactgtc tggccctgtc acaaggtcca gcacctccat acccccttta
     4021 ataagcagtt tgggaacggg tgcgggtctt actccgccca tcccgcccct aactccgccc
     4081 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     4141 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggctgcatt
     4201 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     4261 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     4321 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     4381 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     4441 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     4501 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     4561 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     4621 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     4681 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     4741 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     4801 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     4861 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     4921 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     4981 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     5041 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     5101 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     5161 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     5221 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     5281 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     5341 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     5401 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     5461 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     5521 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     5581 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     5641 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     5701 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     5761 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     5821 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     5881 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     5941 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     6001 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     6061 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     6121 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     6181 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     6241 cctttcgt
//
