LOCUS       sequence-263529-        4654 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expressing vdh gene (for the enzyme transforming benzaldehyde to 
            benzoate) under control of the constitutive promoter J23101, and RBS
            B0032.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4654)
  AUTHORS   Pandi A, Koch M, Voyvodic PL, Soudier P, Bonnet J, Kushwaha M, 
            Faulon JL
  TITLE     Metabolic perceptrons for neural computing in biological systems.
  JOURNAL   Nat Commun. 2019 Aug 28;10(1):3880. doi: 10.1038/s41467-019-11889-0.
  PUBMED    31462649
REFERENCE   2  (bases 1 to 4654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-08-28"; volume: "10(1):3880. doi"; pages: " 
            10.1038/s41467-019-11889-0"
COMMENT     Sequence Label: pSB4C5_J23101-vdh
FEATURES             Location/Qualifiers
     source          1..4654
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1045..1704
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1111..1130)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        1844..1878
                     /label=BBa_J23101
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.7"
     terminator      3453..3510
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     terminator      3577..3671
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      4163..4385
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             join(4433..4654,1..729)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELTTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
ORIGIN
        1 agagagctga cgacgagaac tgtggaaatc aggaatcctt tggttaaagg ctttgagatt
       61 ttccagtgga caaactatgc caagttctca agcgaaaaat tagaattagt ttttagtgaa
      121 gagatattgc cttatctttt ccagttaaaa aaattcataa aatataatct ggaacatgtt
      181 aagtcttttg aaaacaaata ctccatgagg atttatgagt ggttattaaa agaactaaca
      241 caaaagaaaa ctcacaaggc aaatatagag attagccttg atgaatttaa gttcatgtta
      301 atgcttgaaa ataactacca tgagtttaaa aggcttaacc aatgggtttt gaaaccaata
      361 agtaaagatt taaacactta cagcaatatg aaattggtgg ttgataagcg aggccgcccg
      421 actgatacgt tgattttcca agttgaacta gatagacaaa tggatctcgt aaccgaactt
      481 gagaacaacc agataaaaat gaatggtgac aaaataccaa caaccattac atcagattcc
      541 tacctacata acggactaag aaaaacacta cacgatgctt taactgcaaa aattcagctc
      601 accagttttg aggcaaaatt tttgagtgac atgcaaagta agtatgatct caatggttcg
      661 ttctcatggc tcacgcaaaa acaacgaacc acactagaga acatactggc taaatacgga
      721 aggatctgag gttcttatgg ctcttgtatc tatcagtgaa gcatcaagac taacaaacaa
      781 aagtagaaca actgttcacc gttacatatc aaagggaaaa ctgtccatat gcacagatga
      841 aaacggtgta aaaaagatag atacatcaga gcttttacga gtttttggtg cattcaaagc
      901 tgttcaccat gaacagatcg acaatgtaac tactagaggt tgatcgggca cgtaagaggt
      961 tccaactttc accataatga aataagatca ctaccgggcg tattttttga gttatcgaga
     1021 ttttcaggag ctaaggaagc taaaatggag aaaaaaatca cgggatatac caccgttgat
     1081 atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc
     1141 tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag
     1201 cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaacgc tcacccggag
     1261 tttcgtatgg ccatgaaaga cggtgagctg gtgatctggg atagtgttca cccttgttac
     1321 accgttttcc atgagcaaac tgaaacgttt tcgtccctct ggagtgaata ccacgacgat
     1381 ttccggcagt ttctccacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc
     1441 tatttcccta aagggtttat tgagaatatg ttttttgtct cagccaatcc ctgggtgagt
     1501 ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacg
     1561 atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat ccaggttcat
     1621 catgccgttt gtgatggctt ccatgtcggc cgcatgctta atgaattaca acagtactgt
     1681 gatgagtggc agggcggggc gtaataatac tagctccggc aaaaaaacgg gcaaggtgtc
     1741 accaccctgc cctttttctt taaaaccgaa aagattactt cgcgtttgcc acctgacgtc
     1801 taagacgata ctgttcgagg atactagagg atgaccccat ctgtttacag ctagctcagt
     1861 cctaggtatt atgctagcta gtagagtcac acaggaaagt agtagatgca caacgttcag
     1921 ctgaaacagg acaacaccgt tgacacctct tctttcgaat ctgctccgaa cgttcacacc
     1981 gttcagctgc tgatccacgg tcagtctgtt gacgcttcta accagatgac cttcaaacgt
     2041 atctctccga tcgacggtca ggttgcttct atcgctgctg ctgctaccct ggctgacgtt
     2101 gacctggcta tcgaatctgc tgctaaagct ttcccgatct ggtctaaact gtctccgacc
     2161 gaacgtcgtc tgcgtctgct gaaagctgct gacctgatgg acgctcgtac cgaccagttc
     2221 atccagatcg gtatgcgtga aaccggttct accgctacct ggtacggctt caacgttcac
     2281 ctcgctgcta acatgctgcg tgaagctgct gctatgacca cccagatgga cggttctctg
     2341 atcccgtctg acgttccggg taacatggct atgggtatcc gtgttccgtg cggtgttgtt
     2401 gttggtatcg ctccgtggaa cgctccggtt atcctgccga cccgtgcact ggctatgccg
     2461 ctggcttgcg gtaacaccgt tgttctgaaa gcttctgaag cttgcccggc tacccagcgt
     2521 ctgatcggtc aggttctgca cgaagctggt ctgggtgacg gtgttgttaa cgttatcacc
     2581 cacgctgctg aagacgcttc tcagatcgtt gaacgtctga tctctcaccc ggctgttaaa
     2641 cgtatcaact tcaccggttc taccaacgtt ggtaaaatca tcgctgaaac cgctgctaaa
     2701 tacctgaaac cggttctgct ggaactgggt ggtaaagctc cggttgttgt tctgaacgaa
     2761 gctgacgttg acgaagctgt taacgctgtt gttttcggtg ctttcttcaa ccagggtcag
     2821 atctgcatgt ctaccgaacg tgttctggtt caggaccgta tcgctgacca gttcatcgaa
     2881 aaactgatcg aaaaaacccg taccatccac gctggtaacc cgaccttcaa aggtcacgtt
     2941 ctgggtgttc tggaatctca gcgtgctgct aaccgtatcc agcacctgct ggaagacgct
     3001 cagtctcagg gtgctgacct gccgctgggt atccacatcc agaacaccac catgcagccg
     3061 accctggttc tgaacatcca gccggaaatg ctgctgtacc gtgaagaatc tttcggtccg
     3121 gtttgcaccg ttcagcgttt caactctgtt gaagaaggta tcgccctggc taacgactct
     3181 gaattcggtc tgtctgctgc tgttttctct caggacatcg ctcaggccct ggacgttgct
     3241 aaacagatcg actccggtat ctgccacatc aacggtgcta ccgttcacga cgaagctcag
     3301 atgccgttcg gtggtaccaa agcttctggt tacggtcgtt tcggttctaa agcttctatc
     3361 gctgaattca ccgaactgcg ttggatcacc atccagaccc agtctcgtca ctacccgatc
     3421 taagcttcgg tgcttaatgc gtcttcctgc agtccggcaa aaaagggcaa ggtgtcacca
     3481 ccctgccctt tttctttaaa accgaaaaga ttacttcgcg ttatgcaggt acgactttct
     3541 gctagagctg tcagaccaag tttacgagct cgcttggact cctgttgata gatccagtaa
     3601 tgacctcaga actccatctg gatttgttca gaacgctcgg ttgccgccgg gcgtttttta
     3661 ttggtgagaa tccaagcact agggacagta agacgggtaa gcctgttgat gataccgctg
     3721 ccttactggg tgcattagcc agtctgaatg acctgtcacg ggataatccg aagtggtcag
     3781 actggaaaat cagagggcag gaactgctga acagcaaaaa gtcagatagc accacatagc
     3841 agacccgcca taaaacgccc tgagaagccc gtgacgggct tttcttgtat tatgggtagt
     3901 ttccttgcat gaatccataa aaggcgcctg tagtgccatt tacccccatt cactgccaga
     3961 gccgtgagcg cagcgaactg aatgtcacga aaaagacagc gactcaggtg cctgatggtc
     4021 ggagacaaaa ggaatattca gcgatttgcc cgagcttgcg agggtgctac ttaagccttt
     4081 agggttttaa ggtctgtttt gtagaggagc aaacagcgtt tgcgacatcc ttttgtaata
     4141 ctgcggaact gactaaagta gtgagttata cacagggctg ggatctattc tttttatctt
     4201 tttttattct ttctttattc tataaattat aaccacttga atataaacaa aaaaaacaca
     4261 caaaggtcta gcggaattta cagagggtct agcagaattt acaagttttc cagcaaaggt
     4321 ctagcagaat ttacagatac ccacaactca aaggaaaagg acatgtaatt atcattgact
     4381 agcccatctc aattggtata gtgattaaaa tcacctagac caattgagat gtatgtctga
     4441 attagttgtt ttcaaagcaa atgaactagc gattagtcgc tatgacttaa cggagcatga
     4501 aaccaagcta attttatgct gtgtggcact actcaacccc acgattgaaa accctacaag
     4561 gaaagaacgg acggtatcgt tcacttataa ccaatacgct cagatgatga acatcagtag
     4621 ggaaaatgct tatggtgtat tagctaaagc aacc
//
