LOCUS       sequence-263553-        6892 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6892)
  AUTHORS   Hu Z, Jin S, Scotto KW
  TITLE     Transcriptional activation of the MDR1 gene by UV irradiation. Role 
            of NF-Y and Sp1.
  JOURNAL   J Biol Chem. 2000 Jan 28;275(4):2979-85.
  PUBMED    10644769
REFERENCE   2  (bases 1 to 6892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2000-01-28"; volume: "275(4)"; pages: "2979-85"
COMMENT     Sequence Label: pMDR1-1202
FEATURES             Location/Qualifiers
     source          1..6892
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    435..569
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(711..728)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(882..1470)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(962..981)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1641..2432,2433..2501))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2264..2283
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2502..2606)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      2633..3088
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2720..2739)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2930..2951
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    3278..3399
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             4794..6446
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(4860..4878)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     intron          6687..6752
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             join(6882..6892,1..10)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gagaaaggta gaagacccca aggactttcc ttcagaattg ctaagttttt tgagtcatgc
       61 tgtgtttagt aatagaactc ttgcttgctt tgctatttac accacaaagg aaaaagctgc
      121 actgctatac aagaaaatta tggaaaaata ttctgtaacc tttataagta ggcataacag
      181 ttataatcat aacatactgt tttttcttac tccacacagg catagagtgt ctgctattaa
      241 taactatgct caaaaattgt gtacctttag ctttttaatt tgtaaagggg ttaataagga
      301 atatttgatg tatagtgcct tgactagaga tcataatcag ccataccaca tttgtagagg
      361 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
      421 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
      481 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
      541 tcatcaatgt atcttatcat gtctggatcc gtcgaccgat gcccttgaga gccttcaacc
      601 cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct
      661 tctttatcat gcaactcgta ggacaggtgc cggcagcgct cttccgcttc ctcgctcact
      721 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
      781 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
      841 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
      901 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
      961 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     1021 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     1081 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     1141 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     1201 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     1261 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     1321 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     1381 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     1441 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     1501 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     1561 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     1621 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     1681 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     1741 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     1801 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     1861 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     1921 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     1981 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     2041 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     2101 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     2161 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     2221 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     2281 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     2341 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     2401 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     2461 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     2521 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     2581 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc
     2641 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     2701 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     2761 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     2821 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     2881 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     2941 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     3001 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     3061 tttaacaaaa tattaacgct tacaatttgc cattcgccat tcaggctgcg caactgttgg
     3121 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc ccaagctacc atgataagta
     3181 agtaatatta aggtacgtgg aggttttact tgctttaaaa aacctcccac acctccccct
     3241 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
     3301 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     3361 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat ggtactgtaa ctgagctaac
     3421 ataacccggg aggcggagct tgcagtgagc cgagatcctg ccactgcact ccagcctggg
     3481 cgacaaagca agactccgtc tcaaaaaaga aaaaagaaag aaaaacaaaa gaaaacttca
     3541 ttgtattgta aggccaagaa caaaatatat caagataagg aaaatttgta gtcaagaata
     3601 gaaaaaaatt atggctttga agtatgagtt atttaaagaa agtggaaaca tcctcagact
     3661 atgcagtaaa aaacaaagtg attttcttct tctaaactta tgcaataaac tgataggtaa
     3721 tatgtgaaag tcatagaatg tagactagag gatacaacaa acctatttcc tctatgttca
     3781 taagaagtaa gaaaagctct gatgtgagtt agcattgctt tacaattttg aattgtgcag
     3841 attgcacgta cttttcctca gtttgaagta aatagtggac aggaaaaaat attaaatgtt
     3901 ggcagtaaat atggaaggaa attacaacta atgtaatatg ctaaaacatg ctatgtttat
     3961 tttactaatt tgaattaaaa tgtaagaatt taaaatgccc tggaaaaaca cgggcattga
     4021 tctgacgtct gaagttttaa aatattacac actttgaaat agcatttgta ccttgaaata
     4081 cctgtctcta tatatttttt aaaacttcct ttttctttca ttccatttat catcaaataa
     4141 aggatgaaca gatgtaactc agaaactgtc aagcatgctg aagaaagacc actgcagaaa
     4201 aatttctcct agccttttca aaggtgttag gaagcagaaa ggtgatacag aattggagag
     4261 gtcggagttt ttgtattaac tgtattaaat gcgaatcccg agaaaatttc ccttaactac
     4321 gtcctgtagt tatatggata tgaagactta tgtgaacttt gaaagacgtg tctacataag
     4381 ttgaaatgtc cccaatgatt cagctgatgc gcgtttctct acttgccctt tctagagagg
     4441 tgcaacggaa gccagaacat tcctcctgga aattcaacct gtttcgcagt ttctcgagga
     4501 atcagcattc agtcaatccg ggccgggagc agtcatctgt ggtgaggctg attggctggg
     4561 caggaacagc gccggggcgt gggctgagca cagccgcttc gctctctttg ccacaggaag
     4621 cctgagctca ttcgagtagc ggctcttcca agctcaaaga agcagaggcc gctgttcgtt
     4681 tcctttaggt ctttccacta aagtcggagt atcttcttcc aaaatttcac gtcttggtgg
     4741 ccgttccaag ctcgagatct aagtaagctt ggcattccgg tactgttggt aaaatggaag
     4801 acgccaaaaa cataaagaaa ggcccggcgc cattctatcc tctagaggat ggaaccgctg
     4861 gagagcaact gcataaggct atgaagagat acgccctggt tcctggaaca attgctttta
     4921 cagatgcaca tatcgaggtg aacatcacgt acgcggaata cttcgaaatg tccgttcggt
     4981 tggcagaagc tatgaaacga tatgggctga atacaaatca cagaatcgtc gtatgcagtg
     5041 aaaactctct tcaattcttt atgccggtgt tgggcgcgtt atttatcgga gttgcagttg
     5101 cgcccgcgaa cgacatttat aatgaacgtg aattgctcaa cagtatgaac atttcgcagc
     5161 ctaccgtagt gtttgtttcc aaaaaggggt tgcaaaaaat tttgaacgtg caaaaaaaat
     5221 taccaataat ccagaaaatt attatcatgg attctaaaac ggattaccag ggatttcagt
     5281 cgatgtacac gttcgtcaca tctcatctac ctcccggttt taatgaatac gattttgtac
     5341 cagagtcctt tgatcgtgac aaaacaattg cactgataat gaattcctct ggatctactg
     5401 ggttacctaa gggtgtggcc cttccgcata gaactgcctg cgtcagattc tcgcatgcca
     5461 gagatcctat ttttggcaat caaatcattc cggatactgc gattttaagt gttgttccat
     5521 tccatcacgg ttttggaatg tttactacac tcggatattt gatatgtgga tttcgagtcg
     5581 tcttaatgta tagatttgaa gaagagctgt ttttacgatc ccttcaggat tacaaaattc
     5641 aaagtgcgtt gctagtacca accctatttt cattcttcgc caaaagcact ctgattgaca
     5701 aatacgattt atctaattta cacgaaattg cttctggggg cgcacctctt tcgaaagaag
     5761 tcggggaagc ggttgcaaaa cgcttccatc ttccagggat acgacaagga tatgggctca
     5821 ctgagactac atcagctatt ctgattacac ccgaggggga tgataaaccg ggcgcggtcg
     5881 gtaaagttgt tccatttttt gaagcgaagg ttgtggatct ggataccggg aaaacgctgg
     5941 gcgttaatca gagaggcgaa ttatgtgtca gaggacctat gattatgtcc ggttatgtaa
     6001 acaatccgga agcgaccaac gccttgattg acaaggatgg atggctacat tctggagaca
     6061 tagcttactg ggacgaagac gaacacttct tcatagttga ccgcttgaag tctttaatta
     6121 aatacaaagg atatcaggtg gcccccgctg aattggaatc gatattgtta caacacccca
     6181 acatcttcga cgcgggcgtg gcaggtcttc ccgacgatga cgccggtgaa cttcccgccg
     6241 ccgttgttgt tttggagcac ggaaagacga tgacggaaaa agagatcgtg gattacgtcg
     6301 ccagtcaagt aacaaccgcg aaaaagttgc gcggaggagt tgtgtttgtg gacgaagtac
     6361 cgaaaggtct taccggaaaa ctcgacgcaa gaaaaatcag agagatcctc ataaaggcca
     6421 agaagggcgg aaagtccaaa ttgtaaaatg taactgtatt cagcgatgac gaaattctta
     6481 gctattgtaa tactgcgatg agtggcaggg cggggcgtaa tttttttaag gcagttattg
     6541 gtgcccttaa acgcctggtt gctacgcctg aataagtgat aataagcgga tgaatggcag
     6601 aaattcgccg gatctttgtg aaggaacctt acttctgtgg tgtgacataa ttggacaaac
     6661 tacctacaga gatttaaagc tctaaggtaa atataaaatt tttaagtgta taatgtgtta
     6721 aactactgat tctaattgtt tgtgtatttt agattccaac ctatggaact gatgaatggg
     6781 agcagtggtg gaatgccttt aatgaggaaa acctgttttg ctcagaagaa atgccatcta
     6841 gtgatgatga ggctactgct gactctcaac attctactcc tccaaaaaag aa
//
